A5058165180- Atherosclerosis and Cardiovascular Diseases
- Adenosine and Purinergic Signaling
- Immune cells in cancer
- Adipose Tissue and Metabolism
- Phagocytosis and Immune Regulation
- Biomarkers in Disease Mechanisms
- Microbial Metabolism and Applications
- Antiplatelet Therapy and Cardiovascular Diseases
- Congenital heart defects research
- Cholesterol and Lipid Metabolism
- Blood Coagulation and Thrombosis Mechanisms
- Pancreatic function and diabetes
- Receptor Mechanisms and Signaling
- Angiogenesis and VEGF in Cancer
- Lymphatic System and Diseases
- Muscle Physiology and Disorders
- Exercise and Physiological Responses
- Cardiac Fibrosis and Remodeling
- Cardiovascular Function and Risk Factors
- interferon and immune responses
- Chemotherapy-induced cardiotoxicity and mitigation
- Protein Kinase Regulation and GTPase Signaling
- Cardiovascular Issues in Pregnancy
- Chemokine receptors and signaling
- Sympathectomy and Hyperhidrosis Treatments
Harvard University
2022-2024
Boston Children's Hospital
2022-2024
Auburn University
2019-2023
Boston Children's Museum
2022
Chinese Academy of Agricultural Sciences
2015
Institute of Animal Sciences
2015
Excess cholesterol accumulation in lesional macrophages elicits complex responses atherosclerosis. Epsins, a family of endocytic adaptors, fuel the progression atherosclerosis; however, underlying mechanism and therapeutic potential targeting Epsins remains unknown. In this study, we determined role macrophage-mediated metabolic regulation. We then developed an innovative method to therapeutically target macrophage with specially designed S2P-conjugated lipid nanoparticles, which encapsulate...
The objective of this study was to investigate the effects immunological challenge on skeletal muscle fiber type conversion piglets. Sixteen Large White weaned barrows (28 ± 3 d, 8.22 0.89 kg BW) were allotted by weight and litter 2 groups: control group lipopolysaccharide (LPS) group. Saline (control) or LPS injected intravenously via a jugular catheter d 1, 3, 5, 7, 9, 11, 13, 15 at an initial dosage 80 μg/kg BW, which increased 30% each subsequent injection. Blood samples collected h...
Endothelial-to-mesenchymal transition (EndMT) is a dynamic process in which endothelial cells acquire mesenchymal properties and turn contribute to tissue remodeling growth. Previously, we found EndMT associated with mitral valve adaptation after myocardial infarction. Furthermore, collected at 6 months post-myocardial infarction expressed the pan-leukocyte marker CD45 markers. Additionally, induced undergo TGF (transforming growth factor)-β1 strongly coexpressed but not CD11b or CD14....
Protein-tyrosine-phosphatase CD45 is exclusively expressed in all nucleated cells of the hematopoietic system but rarely endothelial cells. Interestingly, our recent study indicated that activation endogenous promoter human colony forming (ECFCs) induced expression multiple EndoMT marker genes. However, detailed molecular mechanisms underlying drive and therapeutic potential manipulation atherosclerosis are entirely unknown.
Metformin, created in 1922, has been the first-line therapy for treating type 2 diabetes mellitus almost 70 years; however, its mechanism of action remains controversial, partly because most prior studies used supratherapeutic concentrations exceeding 1 mM despite therapeutical blood metformin being less than 40 μM. Here we report metformin, at 10-30 μM, blocks high glucose-stimulated ATP secretion from hepatocytes mediating antihyperglycemic action. Following glucose administration, mice...
Small molecular chemicals that target individual subtype of G proteins including Gs, Gi and Gq has been lacking, except for pertussis toxin validated as a selective peptide inhibitor the protein. Recently, cyclic depsipeptide compound termed YM‐254890 was isolated from culture broth Chromobacterium sp., which reported protein by blocking GDP exchange GTP on α subunit complex; however, functional selectivity towards various remains unclear, primarily due to its restricted availability before...
Background: The anti-phagocytic molecule CD47 is upregulated in the atherosclerotic plaques of patients with cerebrovascular disease; however, molecular mechanisms responsible for development and progression lesions have not been fully established. In this study, we postulate how endothelial-specific promotes endothelial-to-mesenchymal transition (EndoMT) potentiating atherosclerosis assess efficacy novel therapeutic interventions cardiovascular disease. Methods: Using single-cell RNA...
Several P2Y nucleotide receptors have been shown to be involved in the early stage of adipocyte differentiation vitro and insulin resistance obese mice; however, exact receptor subtype(s) its underlying molecular mechanism relevant human cells are unclear. Here, using primary visceral preadipocytes as a model, we found that during preadipocyte-to-mature differentiation, P2Y2 (P2Y2R) was most upregulated subtype among eight known only one further dramatically after inflammatory TNFα...
<b>Abstract ID 87958</b> <b>Poster Board 264</b> CD45 is a marker of hematopoietic cells, which rarely expressed in endothelial cells (EC). However, recent studies indicated that indispensable driving the endothelial-to-mesenchymal transition (EndMT) following sheep myocardial infarction. EC activation hallmark initiation and atheroma progression; EndMT has also been implicated pathogenesis atherosclerosis. Here, we aim to investigate whether contributes progression atherosclerosis by...
Introduction: Protein-tyrosine-phosphatase CD45 is exclusively expressed in all nucleated cells of the hematopoietic system but rarely endothelial (ECs). However, a recent study indicated that activation endogenous promoter human colony forming induced expression EndMT marker genes. Also, phosphatase inhibitor blocked activities TGFβ1-treated ovine mitral valve cells. contributes to atherosclerotic pathobiology and associated with more complex plaques. We identified CD45-positive ECs...
Objective It has been known that insulin resistance could lead to hyperglycemia and diabetes; however, the exact cause effect relationship between is not fully understood. Since over nutrition often leads energy excess ATP major cellular source, we hypothesized abnormal high level of extracellular glucose promotes skeletal muscle cells release ATP, which in turn activates purinergic P2Y2 receptor, resulting resistance. Methods Results Real‐time RT‐PCR analysis indicated receptor mRNA was...
ABSTRACT Small molecular chemicals targeting individual subtype of G proteins including Gs, Gi/o and Gq has been lacking, except for pertussis toxin being an established selective peptide inhibitor the protein. Recently, a cyclic depsipeptide compound YM-254890 isolated from culture broth Chromobacterium sp . was reported as protein by blocking GDP exchange GTP on α subunit complex. However, functional selectivity towards various not fully characterized, primarily due to its restricted...
It has been known that obesity is associated with inflammation and insulin resistance adipose tissue contributes to sensitivity glucose homeostasis; however, the exact mechanism(s) of in remains incompletely understood. We hypothesized promotes adipocyte P2Y2 receptor (P2Y2R) induction, leading by inhibiting insulin-AKT signaling pathway. Real-time RT-PCR revealed P2Y2R mRNA was significantly upregulated during human visceral pre-adipocytes differentiation into mature adipocytes vitro....
Introduction: Endothelial to Mesenchymal transition (EndMT) is an intricate process in which endothelial cells (ECs) loosen their cell-cell junctions, migrate into tissue matrix, and acquire mesenchymal phenotype. During embryonic development, EndMT essential yet re-occurrence postnatally associated with cardiovascular diseases. In our previous study, we showed that CD45, a pan-leukocyte marker, expressed mitral valve endothelium at 6 months post-myocardial infarction. Additionally,...
Background: The anti-phagocytic molecule CD47 is upregulated in atherosclerotic plaques of patients with cerebrovascular disease. However, the role endothelial cell-specific pathogenesis atherosclerosis has not yet been defined. Kojima et.al . described atheroprotective effect anti-CD47 antibody by enhancing efferocytosis atherosclerosis. In this study, we determined whether endothelialCD47 regulates Methods: Susceptibility to was evaluated cross sections aortic sinus mice. Using single-cell...
Abstract The forkhead box O1 (FOXO1) transcription factor plays critical roles in regulating not only metabolic activity but also angiogenesis the vascular endothelium 1–4 . Our previous studies show that epsin endocytic adaptors can regulate both and lymphangiogenesis 5–7 Endothelial cells (ECs) lining inside of blood vessels are continuously exposed to circulating insulin insulin-like growth factors (IGFs). Emerging evidences suggest ECs affect β-cell function 8–11 Excessive IGF2,...
Introduction: Atherosclerosis is the leading cause of mortality in United States and most other developed countries. Endothelial cell (EC) activation a hallmark initiation progression atherosclerosis; identification molecular markers dysfunctional endothelium represents biological challenge to developing new therapeutic targets. CD45 marker hematopoietic cells, which rarely expressed ECs. However, recent studies indicated that indispensable driving endothelial-to-mesenchymal transition...
Recently we reported that in human coronary artery endothelial cells, activation of the P2Y2 receptor induces up‐regulation tissue factor (TF), a key initiator coagulation cascade. However, it has been shown by others monocyte TF is more important than terms provoking pro‐thrombotic state. Thus, aimed to study whether monocytes express and its role control expression. RT‐PCR activity assays revealed among eight P2Y nucleotide receptors, subtype was selectively functionally expressed...
Obesity is a multifactorial chronic condition that increases the risk of cardiovascular diseases, type II diabetes, and certain types cancer. It has been known some chemokines their receptors facilitate development obesity, leading to other related comorbidities. However, exact role atypical chemokine receptor CXCR7 in obesity obesity-induced diabetes remains unknown. We aimed study whether promotes adipogenesis insulin resistance human visceral adipocytes vitro. Using RT-PCR flow cytometry...
ABSTRACT BACKGROUND Excess cholesterol accumulation in lesional macrophages elicits complex responses atherosclerosis. Epsins, a family of endocytic adaptors, fuel the progression atherosclerosis; however, underlying mechanism and therapeutic potential targeting Epsins remains unknown. In this study, we determined role macrophage-mediated metabolic regulation. We then developed an innovative method to therapeutically-target macrophage with specially-designed S2P-conjugated lipid...
Background: Chronic liver disease, such as non-alcoholic fatty disease (NAFLD), is one of the most prevalent diseases, with 4.5 million people diagnosed in U.S. 2018. If untreated, fibrosis-induced cirrhosis will eventually develop into hepatocellular carcinoma. Epsins are highly conserved endocytic adaptor proteins, participating selected membrane receptor endocytosis. Globally disruption both epsin 1 and 2 induced embryonic lethality by Notch signaling pathway, which has recently been...