A5037817807- Cancer, Hypoxia, and Metabolism
- Metabolomics and Mass Spectrometry Studies
- Glioma Diagnosis and Treatment
- Drug Transport and Resistance Mechanisms
- Liver Disease Diagnosis and Treatment
- Atomic and Subatomic Physics Research
- Amino Acid Enzymes and Metabolism
- Advanced NMR Techniques and Applications
- Advanced MRI Techniques and Applications
- Pituitary Gland Disorders and Treatments
- Metal complexes synthesis and properties
- Advanced Chemical Sensor Technologies
- Cancer Research and Treatments
- Mitochondrial Function and Pathology
- Adrenal and Paraganglionic Tumors
- Crystallization and Solubility Studies
- X-ray Diffraction in Crystallography
- Immune cells in cancer
- Meningioma and schwannoma management
- Crystal structures of chemical compounds
- Crystallography and molecular interactions
- Magnetism in coordination complexes
- Radiopharmaceutical Chemistry and Applications
- Diet and metabolism studies
- Metabolism and Genetic Disorders
Houston Methodist
2017-2024
Methodist Hospital
2017-2024
Cornell University
2024
University of Winnipeg
2014-2019
National Research Council Institute for Biodiagnostics
2008-2014
National Academies of Sciences, Engineering, and Medicine
2009-2010
Gulbarga University
2002-2009
National Research Council Canada
2009
Centre of Biomedical Research
2008
Sanjay Gandhi Post Graduate Institute of Medical Sciences
2005-2006
Abstract A major rate-limiting step in developing more effective immunotherapies for GBM is our inadequate understanding of the cellular complexity and molecular heterogeneity immune infiltrates gliomas. Here, we report an integrated analysis 201,986 human glioma, immune, other stromal cells at single cell level. In doing so, discover extensive spatial infiltrates. We identify signatures nine distinct myeloid subtypes, which five are independent prognostic indicators glioma patient survival....
Electromagnetic fields (EMF) raise intracellular levels of reactive oxygen species (ROS) that can be toxic to cancer cells. Because weak magnetic influence spin state pairing in redox-active radical electron pairs, we hypothesize they disrupt flow the mitochondrial transport chain (ETC). We tested this hypothesis by studying effects oscillating (sOMF) produced a new noninvasive device involving permanent magnets spinning with specific frequency and timing patterns. studied sOMF on ETC...
Abstract The unconjugated bile acids cholic acid, deoxycholic and chenodeoxycholic acid; their glycine taurine conjugated glycocholic glycodeoxycholic glycochenodeoxycholic taurocholic taurodeoxycholic taurochenodeoxycholic a ursodeoxycholic tauroursodeoxycholic were characterized through 1 H 13 C NMR in aqueous media under the physiological pH region (7.4±0.1). Assignments of signals all made using combination several one‐ two‐dimensional, homonuclear ( H− H) heteronuclear C) correlations...
Background: Glioblastoma (GBM) can use metabolic fuels other than glucose (Glc). The ability of GBM to galactose (Gal) as a fuel via the Leloir pathway is investigated. Methods: Gene transcript data were accessed determine association between expression genes and patient outcomes. Growth studies performed on five primary patient-derived cultures using Glc-free media supplemented with Gal. role Glut3/Glut14 in sugar import was investigated antibody inhibition hexose transport. A specific...
Abstract 1 H and 13 C NMR spectra of intact human bile were assigned using one‐dimensional ( C) two‐dimensional H‐ experiments. Individual conjugated acids—glycocholic acid, glycodeoxycholic glycochenodeoxycholic taurocholic taurodeoxycholic taurochenodeoxycholic acid—were identified. The acids quantified accurately individually in a single step by distinct characteristic amide signals. Making use NMR, the study also suggests way to analyze unconjugated separately, if present. Chemical shift...
Abstract A simple method for quantification of conjugated bile acids in human using 1 H NMR spectroscopy is presented. Bile are essentially with either glycine or taurine. The amide NH resonances from the invariably devoid interfering signals spectra. Under physiologic conditions (pH ∼7.0 to 7.7), signal intensities attenuated due chemical exchange and hence quantitative estimation precluded. In present study, quantity total taurine could be obtained accurately by suppressing reducing pH...
Human gallbladder bile dissolved in dimethylsulfoxide provides sharp and resolved signals for major components 1H NMR spectra. Characteristic well-resolved marker that invariably appear spectra of were identified cholesterol (H18 methyl signal at 0.643 ppm), lipids (glycerol CH 5.064 total acids the range 0.520-0.626 glycine conjugated (NH 6.958 taurine 7.646 urea (NH2 near 5.48 which enabled their rapid accurate analysis. Excellent linearity precision quantitative analysis was observed all...
The utility of (1)H MR spectroscopy in detecting chronic cholestasis has been investigated. amide proton region the spectrum human bile plays a major role differentiating cholestatic (Ch) patterns from normal ones. Bile obtained ducts contains both taurine and glycine conjugates acids--cholic acid (CA), chenodeoxycholic (CDCA), deoxycholic (DCA). Absence glycine-conjugated glycochenodeoxycholic (GCDCA) observed samples primary sclerosing cholangitis (PSC) patients. A total 32 patients with...
Objectives Lung cancer is one of the most lethal cancers. Currently, there are no biomarkers for early detection, monitoring treatment response, and detecting recurrent lung cancer. We undertook this study to determine if 1 H magnetic resonance spectroscopy (MRS) sputum exhaled breath condensate (EBC), as a noninvasive tool, can identify metabolic Materials Methods Sputum EBC samples were collected from 20 patients, comprising patients with pathologically confirmed non-small cell ( n = 10)...
A phenol based novel macrocyclic binucleating compartmental ligand N,N-bis(2,6-diiminomethyl-4-methyl-1-hydroxyphenyl)malonoyldicarboxamide was prepared. The complexes were prepared by template method reacting 2,6-diformyl-4-methylphenol, malonoyl dihydrazide and the metal chlorides of Cu(II), Ni(II), Co(II), Cd(II), Zn(II) Hg(II) in methanol to get a series dinuclear complexes. characterized elemental analyses, conductivity measurements, magnetic susceptibility data, IR, UV-Vis, ESR, NMR...
We postulate that meningiomas undergo distinct metabolic reprogramming in tumorigenesis and unraveling their phenotypes provide new therapeutic insights. Glutamine catabolism is key to the growth proliferation of tumors. Here, we investigated metabolomics freshly resected glutamine metabolism patient-derived meningioma cells.
Abstract Pituitary adenomas (PAs) are benign growths arising from epithelial cells in the adenohypophysis of pituitary gland. To date, there has been no detailed metabolic characterization PAs various subtypes. In this study, we report nuclear magnetic resonance (NMR) based metabolomic analysis surgically resected tumors forty five tumor patients [gonadotropic (LH/FSH-secreting) = 17; prolactinomas (PRL-secreting) 11, Cushing’s disease (ACTH-secreting) 4, non-functional 5, and mixed 8] who...
End-off compartmental pentadentate Schiff base, 2,6-bis[3′-methyl-2′-carboxamidyliminomethyl(6′,7′)benzindole]-4-methylphenol is synthesized and characterized by 2D NMR experiments mass spectral techniques. The homodinuclear phenalato bridged end-off Schiff-base complexes Cu(II), Co(II), Ni(II), Mn(II), Fe(III), VO(IV), Zn(II), Cd(II) Hg(II) have been prepared the template method using precursors 2,6-diformyl-4-methylphenol, 3-methyl(6′,7′)-2-benzindolehydrazide metal chlorides in 1 : 2...
People with inflammatory bowel disease (IBD) are at risk for developing colorectal cancer, and this increases a rate of 1% per year after 8-10 years having the disease. Saturated omega-6 polyunsaturated fatty acids (PUFAs) have been implicated in its causation. Conversely, omega-3 PUFAs may potential to confer therapeutic benefit. Since proton magnetic resonance spectroscopy ((1)H MRS) combined pattern recognition methods could be valuable adjunct histology, objective study was analyze (1)H...
Primary sclerosing cholangitis (PSC) has been considered to be either an “autoimmune disease” or a “bile acid‐induced injury.” In vitro MRS studies on PSC patients have limitations due the contamination of bile with contrast agent (commonly administered during endoscopic retrograde cholangiopancreatography) and/or use patient cohorts other diseases as controls. The objective this study was quantify biliary metabolites using in vivo 1 H and gain insight into pathogenesis PSC. Biliary 10 14...
Glioblastoma (GBM) uses Glut3 and/or Glut14 and the Leloir pathway to catabolize D-Galactose (Gal). UDP-4-deoxy-4-fluorogalactose (UDP-4DFG) is a potent inhibitor of two key enzymes, UDP-galactose-4-epimerase (GALE) UDP-Glucose 6-dehydrogenase (UGDH), involved in Gal metabolism glycan synthesis. The antimetabolite 4-deoxy-4-fluorogalactose (4DFG) good substrate for Glut3/Glut14 acts as glioma chemotherapeutic.
A compartment ligand 2,6-bis[5′-chloro-3′-phenyl-1H-indole-2′-carboxamidyliminomethyl]-4-methylphenol was prepared and homobinuclear phenol-bridged Cu(II), Ni(II), Co(II), Zn(II), Cd(II), Hg(II), Fe(III), Mn(II) complexes have been by the template method using precursors 2,6-diformyl-4-methylphenol, 5-chloro-3-phenylindole-2-carbohydrazide metal chlorides in 1 : 2 ratio, respectively. The were characterized elemental analyses, conductivity measurements, magnetic susceptibility data, IR, NMR,...