A5087510269- Pneumocystis jirovecii pneumonia detection and treatment
- Tuberculosis Research and Epidemiology
- Microbial Metabolic Engineering and Bioproduction
- Microbial Metabolism and Applications
- Biocrusts and Microbial Ecology
- Plant and Biological Electrophysiology Studies
- Immunodeficiency and Autoimmune Disorders
- Carbohydrate Chemistry and Synthesis
- 3D Printing in Biomedical Research
- Enzyme Structure and Function
- Mycobacterium research and diagnosis
- Phenothiazines and Benzothiazines Synthesis and Activities
- Microbial Natural Products and Biosynthesis
- Chemical Synthesis and Analysis
- Biochemical Acid Research Studies
- Biochemical and Molecular Research
Ecovative Design (United States)
2021
Wadsworth Center
1990-2011
New York State Department of Health
1990-2011
Adenosine 3',5'-cyclic monophosphate (cAMP)-mediated signal transduction is common in both prokaryotes and eukaryotes, several bacterial pathogens modulate cAMP signaling pathways of their mammalian hosts during infection. In this study, levels associated with Mycobacterium tuberculosis bovis BCG were measured macrophage within bacteria increased c. 50-fold infection J774.16 macrophages, relative to the incubated tissue culture media alone. also cytoplasm upon uptake live, but not dead,...
The native architectures of the pyruvate and 2-oxoglutarate dehydrogenase complexes have been investigated by cryoelectron microscopy unstained, frozen-hydrated specimens. In complex transacylase (E2) components exist as 24-subunit, cube-shaped assemblies that form structural cores complexes. Multiple copies (12-24) alpha-ketoacid (E1) dihydrolipoyl (E3) bind to surface cores. Images enzyme do not appear consistent with a symmetric arrangement E1 E3 subunits about octahedrally E2 core. Often...
Summary Mycobacterium tuberculosis CRP Mt , encoded by Rv3676 ( crp ), is a CRP‐like transcription factor that binds with the serC –Rv0885 intergenic region. In present study, we evaluated 's regulation of and Rv0885 in M. bovis BCG, using site‐specific mutagenesis, promoter fusions reverse‐transcriptase PCR (RT‐PCR). The binding site was required for full expression Rv0885, both genes reduced BCG mutants. These data show directly activates expression. restored ability an Escherichia coli...
Mycobacterium tuberculosis Rv3676 encodes a cyclic AMP (cAMP) receptor-like protein (CRP(Mt)) that has been implicated in global gene regulation and may play an important role during infection. The CRP(Mt) ortholog bovis BCG, CRP(BCG), is dysfunctional Escherichia coli CRP competition assay proposed as potential source of M. BCG's attenuation. We compared CRP(BCG) vitro vivo, BCG tuberculosis, to evaluate CRP(BCG)'s function mycobacterial system. Both proteins formed dimers lysates, bound...
A novel screening method for antimycobacterial agents using Mycobacterium marinum was developed. M. selected as a model organism because it has close phylogenetic relationship to tuberculosis, relatively rapid doubling time, similar drug susceptibilities and less stringent safety requirements. More than 1000 crude marine plant extracts were screened against in Zone of Inhibition (ZOI) assay, twenty-one target identified. The organic extract the sponge, Haliclona sp.10, chosen further...