A5060191388- CRISPR and Genetic Engineering
- RNA and protein synthesis mechanisms
- Genomics and Phylogenetic Studies
- Microbial Metabolic Engineering and Bioproduction
- DNA and Biological Computing
- Bacterial Genetics and Biotechnology
- Gene Regulatory Network Analysis
- Amino Acid Enzymes and Metabolism
- Chromosomal and Genetic Variations
- Molecular Biology Techniques and Applications
- Polyamine Metabolism and Applications
- Advanced biosensing and bioanalysis techniques
- Animal Virus Infections Studies
- Herpesvirus Infections and Treatments
- DNA Repair Mechanisms
- Plant and Biological Electrophysiology Studies
- Biocrusts and Microbial Ecology
- 3D Printing in Biomedical Research
Columbia University
2018-2022
New York Proton Center
2021
The University of Texas at Austin
2014-2015
Significance This paper presents a library preparation method that dramatically improves the error rate associated with high-throughput DNA sequencing and is substantially more cost-effective than existing error-correction methods. In this strategy, templates are circularized, copied multiple times in tandem rolling circle polymerase, then sequenced on any machine. Each read produced computationally processed to obtain consensus sequence of all linked copies original molecule. Because it...
The maintenance of chromosomal integrity is an essential task every living organism and cellular repair mechanisms exist to guard against insults DNA. Given the importance this process, it expected that DNA proteins would be evolutionarily conserved, exhibiting very minimal sequence change over time. However, BRCA1, gene involved in repair, has been reported evolving rapidly despite fact many protein-altering mutations within convey a significantly elevated risk for breast ovarian cancers....
Cell-free expression systems enable rapid prototyping of genetic programs in vitro. However, current throughput cell-free measurements is limited by the use channel-limited fluorescent readouts. Here, we describe DNA Regulatory element Analysis cell-Free Transcription and Sequencing (DRAFTS), a robust vitro approach for multiplexed measurement transcriptional activities from thousands regulatory sequences single reaction. We employ this method active cell lysates developed ten diverse...
Over evolutionary time, both host- and virus-encoded genes have been continually selected to modify their interactions with one another. This has resulted in the rapid evolution of specific codons that govern physical between host virus proteins. Virologists discovered these signatures, acquired nature, can provide a shortcut functional dissection host-virus laboratory. However, use studies this way is complicated by fact many nonhuman primate species are endangered, biomaterials often...
Major genomic deletions in independent eukaryotic lineages have led to repeated ancestral loss of biosynthesis pathways for nine the twenty canonical amino acids. While evolutionary forces driving these polyphyletic deletion events are not well understood, consequence is that extant metazoans unable produce essential acids (EAAs). Previous studies highlighted EAA tends be more energetically costly, raising possibility were lost from organisms with access abundant EAAs. It unclear whether...
Major genomic deletions in independent eukaryotic lineages have led to repeated ancestral loss of biosynthesis pathways for nine the twenty canonical amino acids 1 . While evolutionary forces driving these polyphyletic deletion events are not well understood, consequence is that extant metazoans unable produce essential (EAAs). Previous studies highlighted EAA tends be more energetically costly 2,3 , raising possibility were lost from organisms with access abundant EAAs environment 4,5 It...
Abstract Cell-free expression systems enable rapid prototyping of genetic programs in vitro . However, current throughput cell-free measurements is often limited by the use single-channel reporter assays. Here, we describe DNA Regulatory element Analysis cell-Free Transcription and Sequencing (DRAFTS), a robust approach for multiplexed measurement transcriptional activities from thousands regulatory sequences single reaction. We employed this method active cell lysates developed ten diverse...