A5016183722- HIV Research and Treatment
- Immune Cell Function and Interaction
- RNA and protein synthesis mechanisms
- T-cell and B-cell Immunology
- RNA modifications and cancer
- RNA Research and Splicing
- SARS-CoV-2 and COVID-19 Research
- Computational Drug Discovery Methods
- Cytokine Signaling Pathways and Interactions
- Diabetes and associated disorders
- Respiratory viral infections research
- Immunotherapy and Immune Responses
- Influenza Virus Research Studies
- vaccines and immunoinformatics approaches
- Reproductive System and Pregnancy
- Monoclonal and Polyclonal Antibodies Research
- Statistical Methods in Clinical Trials
- Viral Infections and Immunology Research
- Cell Image Analysis Techniques
- Advanced Chemical Sensor Technologies
- Biosensors and Analytical Detection
- HIV-related health complications and treatments
- Immune Response and Inflammation
- Advanced Biosensing Techniques and Applications
- Cancer-related molecular mechanisms research
Massachusetts Institute of Technology
2025
MIT Lincoln Laboratory
2023-2024
Ragon Institute of MGH, MIT and Harvard
2013-2023
Harvard University
2020
Whitehead Institute for Biomedical Research
2020
Brigham and Women's Hospital
2020
Increased IFN-α production contributes to the pathogenesis of infectious and autoimmune diseases. Plasmacytoid dendritic cells (pDCs) from females produce more upon TLR7 stimulation than pDCs males, yet mechanisms underlying this difference remain unclear. In article, we show that basal levels IFN regulatory factor (IRF) 5 in were significantly higher compared with males positively correlated percentage IFN-α-secreting pDCs. Delivery recombinant IRF5 protein into human primary increased...
Multiple studies have broadened the roles of natural killer (NK) cells functioning as purely innate lymphocytes by demonstrating that they are capable putative antigen-specific immunological memory against multiple infectious agents including HIV-1 and influenza. However, mechanisms underlying antigen specificity remain unknown. Here, we demonstrate human NK cell develops upon exposure to both HIV influenza, unified a conserved epitope-specific targetable mechanism largely dependent on...
Understanding the interactions between SARS-CoV-2 and human immune system is paramount to characterization of novel variants as virus co-evolves with host. In this study, we employed state-of-the-art molecular docking tools conduct large-scale virtual screens, predicting binding affinities 64 cytokines against 17 nucleocapsid proteins from six betacoronaviruses. Our comprehensive in silico analyses reveal specific changes cytokine-nucleocapsid protein interactions, shedding light on...
Increasing data suggest that NK cells can mediate antiviral activity in HIV-1-infected humans, and as such, novel approaches harnessing the anti-HIV-1 function of both T represent attractive options to improve future HIV-1 immunotherapies. Chronic progressive infection has been associated with a loss CD4(+) helper cell accumulation anergic cells. As several studies have suggested cytokines produced by are required enhance various models, we hypothesized reconstitution HIV-1-specific T-cell...
ABSTRACT For over a decade, multiple studies have disputed the notion of natural killer (NK) cells as purely innate lymphocytes by demonstrating that they are capable putative antigen-specific immunological memory against infectious agents including two critical global health priorities – HIV and influenza. However, mechanisms underlying antigen specificity remain unknown. Herein, we demonstrate human NK cell develops upon exposure to both influenza, unified conserved epitope-specific...
Drug repurposing can quickly and cost-effectively identify medical countermeasures against pathogens with pandemic potential could be used as a down-selection method for selecting US Food Administration-approved drugs to test in clinical trials. We compared results from 15 high-throughput vitro screening efforts that tested approved clinically evaluated activity SARS-CoV-2 replication. From the studies, 304 were identified displaying highest level of confidence individual screens. Of those...
Abstract The United States Department of Agriculture has recently released reports that show samples from 2022-2024 highly pathogenic avian influenza (H5N1) have been detected in mammals and birds (1). To date, the Centers for Disease Control there 27 humans infected with H5N1 2024 (2). broader potential impact on human health remains unclear. In this study, we computationally model 1,804 protein complexes consisting various H5 isolates 1959 to against 11 hemagglutinin domain 1...
Abstract Evidence suggests natural killer (NK) cells can mediate antiviral activity in HIV-infected humans. Here, we sought to investigate whether the level of NK cell activation primary HIV infection not only influences mediated elimination infected cells, but also affects longevity and effectiveness HIV-specific T responses. To address this hypothesis, monitored viral loads adaptive immune responses a humanized BLT mouse model infection, comparing cell-depleted control mice. Depletion was...
Abstract Background: Differential sex-dependent immune activation is a common feature in both HIV-1 and autoimmune diseases. Plasmacytoid dendritic cells (pDCs) derived from females have been shown to produce significantly more IFNα response HIV-1-encoded TLR7/8 ligands than pDCs males, resulting stronger secondary T cell activation. Given their crucial role the regulation of production by pDCs, we investigated impact interferon regulatory factors (IRF) on observed differences. Methods:...
Abstract Breath contains numerous classes of compounds and biomolecules that could potentially be used as biomarkers for infectious disease well a range other respiratory conditions or states. A testbed simultaneous, multi-modal measurements was developed. Seventeen healthy subjects provided breath samples at baseline repiratory rate particle size, lipid composition bacterial nucleic acid analysis. The majority the particles participants exhaled were smaller than 5 μm, consistent with...
Understanding the interactions between SARS-CoV-2 and human immune system is paramount to characterization of novel variants as virus co-evolves with host. In this study, we employed state-of-the-art molecular docking tools conduct large-scale virtual screens, predicting binding affinities 64 cytokines against 17 nucleocapsid proteins from six betacoronaviruses. Our comprehensive in silico analyses reveal specific changes cytokine-nucleocapsid protein interactions, shedding light on...