A5013879236- Hormonal Regulation and Hypertension
- Adrenal and Paraganglionic Tumors
- Adrenal Hormones and Disorders
- Pituitary Gland Disorders and Treatments
- Cardiovascular, Neuropeptides, and Oxidative Stress Research
- Blood Pressure and Hypertension Studies
- Cancer, Hypoxia, and Metabolism
- Ion Transport and Channel Regulation
- Gout, Hyperuricemia, Uric Acid
- Growth Hormone and Insulin-like Growth Factors
- Estrogen and related hormone effects
- Pharmacological Effects and Assays
- Renal and Vascular Pathologies
- Cardiac Imaging and Diagnostics
- Eicosanoids and Hypertension Pharmacology
- Neuroblastoma Research and Treatments
- Cardiac, Anesthesia and Surgical Outcomes
- Pharmacogenetics and Drug Metabolism
- Quantum, superfluid, helium dynamics
- Renin-Angiotensin System Studies
- Cancer, Lipids, and Metabolism
- Electrochemical sensors and biosensors
- Glioma Diagnosis and Treatment
- Spacecraft and Cryogenic Technologies
- Thyroid Disorders and Treatments
Tohoku University Hospital
2016-2025
Tohoku University
2015-2024
Hypertension Institute
2023
University of Michigan
2015-2019
G.V. (Sonny) Montgomery VA Medical Center
2014-2017
University of Mississippi Medical Center
2014-2017
Tohoku Medical and Pharmaceutical University
2015
Jackson Memorial Hospital
2014-2015
ASKA Pharmaceutical (Japan)
2015
Nippon Veterinary and Life Science University
2015
Approximately half of patients with primary aldosteronism (PA) have clinically evident disease according to clinical (hypertension) and/or laboratory (aldosterone and renin levels) findings but do not nodules detectable in routine cross-sectional imaging. However, the detailed histopathologic, steroidogenic, pathobiological features image–negative PA are controversial. To examine histopathology, steroidogenic enzyme expression, aldosterone-driver gene somatic mutation status...
Adrenal venous sampling is currently the only reliable method to distinguish unilateral from bilateral diseases in primary aldosteronism. In this study, we attempted determine whether peripheral plasma levels of 18-oxocortisol (18oxoF) and 18-hydroxycortisol could contribute clinical differentiation between aldosteronoma hyperaldosteronism 234 patients with aldosteronism, including computed tomography (CT)–detectable (n=113) (n=121), all whom underwent CT adrenal sampling. All aldosteronomas...
In primary aldosteronism (PA), glomerular hyperfiltration due to excessive aldosterone is considered underestimate actual renal damage.Our objectives were determine the prevalence of chronic kidney disease (CKD) in PA and identify predictors decreasing estimated filtration rate (eGFR) after treatment.This was a 12-month prospective study patients with treated at Tohoku University Hospital.All according results adrenal venous sampling; 102 aldosterone-producing adenoma underwent...
Abstract Context Primary aldosteronism is a form of low-renin hypertension characterized by dysregulated aldosterone production. Objective To investigate the contributions renin-independent and ACTH-mediated in individuals with phenotype representing entire continuum blood pressure. Design/Participants Human physiology study 348 participants severe and/or resistant hypertension, hypokalemia, elevated pressure stage I/II normal Setting 4 international centers. Interventions/Main Outcome...
Adrenal vein sampling (AVS) is fundamental for subtype diagnosis in patients with primary aldosteronism. AVS protocols vary between centers, especially diagnostic indices and use of adrenocorticotropic hormone (ACTH) stimulation. We investigated the role both continuous ACTH infusion bolus on performance interpretation a sample 76 confirmed In 36 aldosteronism patients, was performed under basal conditions after infusion, 40 IV bolus. Both determined an increase rate successful cannulation...
Adrenal venous sampling (AVS) is critical to determine the subtype of primary aldosteronism (PA). Central AVS (C-AVS)--that is, collection effluents from bilateral adrenal central veins (CV)--sometimes does not allow differentiation between aldosterone-producing adenomas (APA) and idiopathic hyperaldosteronism. To establish best treatment course, we have developed segmental (S-AVS); that collect tributaries CV intra-adrenal sources aldosterone overproduction. We then evaluated clinical...
Abstract Context Results of previous studies demonstrated clear racial differences in the prevalence somatic mutations among patients with aldosterone-producing adenoma (APA). For instance, those East Asian countries have a high KCNJ5, whereas other aldosterone-driving genes are rare. Objectives To determine mutation Japanese APA using an aldosterone synthase (CYP11B2) immunohistochemistry (IHC)-guided sequencing approach. Method Patients unilateral form primary aldosteronism who underwent...
To use segmental adrenal venous sampling (AVS) (S-AVS) of effluent tributaries (a version AVS that, in addition to helping identify aldosterone hypersecretion, also enables the evaluation intra-adrenal hormone distribution) detect and localize secretion.The institutional review board approved this study, all patients provided informed consent. S-AVS was performed 65 with primary aldosteronism (34 men; mean age, 50.9 years ± 11 [standard deviation]). A microcatheter inserted first-degree...
Aldosterone-producing adenoma is a major subtype of primary aldosteronism. The number cases these adenomas, which are below the detection limit computed tomography but diagnosed by adrenal venous sampling, has recently been increasing. However, pathophysiology especially those manifesting clinically overt hyperaldosteronism despite their small size, remains unknown. Therefore, we examined correlation between tumor size and status intratumoral steroidogenic enzymes involved in aldosterone...
Measurement of plasma aldosterone and renin concentration, or activity, is useful for selecting antihypertensive agents detecting hyperaldosteronism in hypertensive patients. However, it takes several days to get results when measured by radioimmunoassay development more rapid assays has been long expected. We have developed chemiluminescent enzyme immunoassays enabling the simultaneous measurement both concentrations 10 minutes a fully automated assay using antibody-immobilized magnetic...
Abstract Context Aldosterone-producing adrenocortical adenomas (APAs) are mainly composed of clear (lipid rich) and compact (eosinophilic) tumor cells. The detailed association between these histological features somatic mutations (KCNJ5, ATP1A1, ATP2B3, CACNA1D) in APAs is unknown. Objective To examine the individual genotypes APAs. Methods Examination 39 subjected to targeted next-generation sequencing (11 KCNJ5, 10 8 quantitative morphological immunohistochemical (CYP11B2 CYP17A1)...
Aldosterone-producing adenomas (APAs) harbor marked intratumoral heterogeneity in terms of morphology, steroidogenesis, and genetics. However, an association biological significance morphologically identified tumor cell subtypes genotypes is virtually unknown. KCNJ5 mutation most frequently detected generally considered a curable phenotype by adrenalectomy. Therefore, to explore the APA based on intracellular hormonal activities, 35 consecutively selected APAs (n=18; mutated, n=17; wild...
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Abstract Context Detecting patients with surgically curable aldosterone-producing adenoma (APA) among hypertensive individuals is clinically pivotal. Liquid chromatography–tandem mass spectrometry (LC-MS/MS) the ideal method of measuring plasma aldosterone concentration (PAC) because inaccuracy conventional chemiluminescent enzyme immunoassay (CLEIA). However, LC-MS/MS expensive and requires expertise. We have developed a novel noncompetitive CLEIA (NC-CLEIA) for PAC in 30 minutes. Objective...
Multiple endocrine neoplasia type 2B (MEN2B) is a rare autosomal dominant disorder caused by germline pathogenic RET variants. On the other hand, Charcot-Marie-Tooth disease (CMT) hereditary neurological disorder, characterized distal muscle weakness and sensory loss, with approximately 100 identified causative genes. Herein, we report de novo mutation in patient presenting clinical features of both MEN2B CMT. The patient, 22-year-old woman, had history lower limb weakness, no family or was...
Purkinje cell protein 4 (PCP4) is a calmodulin (CaM)-binding that accelerates calcium association and dissociation with CaM. It has been previously detected in aldosterone-producing adenomas (APA), but details on its expression function adrenocortical tissues have remained unknown. Therefore, we performed the immunohistochemical analysis of PCP4 following tissues: normal adrenal (NA; n =15), APA ( cortisol-producing idiopathic hyperaldosteronism cases (IHA; =5). samples =45) were also...
Primary aldosteronism due to unilateral aldosterone-producing adenoma (APA) is a surgically curable form of hypertension. Bilateral APA can also be in theory but few successful cases found the literature. It has been reported that even using adrenal venous sampling (AVS) via bilateral central veins, it extremely difficult differentiate from idiopathic hyperaldosteronism (IHA) harbouring computed tomography (CT)-detectable adrenocortical nodules. We report case diagnosed by segmental AVS...
Pheochromocytomas (PHEOs) are relatively rare catecholamine-producing tumors derived from adrenal medulla. Tumor microenvironment (TME) including neoangiogenesis have been explored in many human neoplasms but not necessarily PHEOs. Therefore, this study, we firstly examined tumor infiltrating lymphocytes (CD4 and CD8), associated macrophages (CD68 CD163), sustentacular cells (S100p) angiogenic markers (CD31 areas of intratumoral hemorrhage) 39 cases PHEOs the quantitative fashion. We then...