A5100757646- Immunotherapy and Immune Responses
- Cancer, Hypoxia, and Metabolism
- Gene expression and cancer classification
- Glioma Diagnosis and Treatment
- RNA Interference and Gene Delivery
- Bioinformatics and Genomic Networks
- Cancer Immunotherapy and Biomarkers
- CAR-T cell therapy research
- Machine Learning in Bioinformatics
- Platelet Disorders and Treatments
- Cancer-related molecular mechanisms research
- Sperm and Testicular Function
- Molecular Biology Techniques and Applications
- Cancer Cells and Metastasis
- Immune cells in cancer
- Mitochondrial Function and Pathology
- Virus-based gene therapy research
- Sirtuins and Resveratrol in Medicine
- Genomics and Chromatin Dynamics
- Nanoplatforms for cancer theranostics
- Testicular diseases and treatments
- Adipokines, Inflammation, and Metabolic Diseases
- Cancer Research and Treatments
- Extracellular vesicles in disease
- Cancer, Lipids, and Metabolism
Cedars-Sinai Medical Center
2009-2025
Zhoushan Hospital
2016-2024
Affiliated Hospital of Qingdao University
2014-2024
Wenzhou Medical University
2023-2024
Qingdao University
2014-2024
Zhejiang University
2008-2023
Henan University
2023
Zhengzhou University
2023
Henan Provincial People's Hospital
2018-2023
Shandong Provincial QianFoShan Hospital
2021
Abstract Glioblastoma multiforme (GBM) is the most aggressive primary brain tumor, with current treatment remaining palliative. Immunotherapies harness body's own immune system to target cancers and could overcome limitations of conventional treatments. One active immunotherapy strategy uses dendritic cell (DC)-based vaccination initiate T-cell-mediated antitumor immunity. It has been proposed that cancer stem-like cells (CSCs) may play a key role in initiation, progression, resistance...
Abstract Motivation: Consensus clustering, also known as cluster ensemble, is one of the important techniques for microarray data analysis, and particularly useful class discovery from data. Compared with traditional clustering algorithms, consensus approaches have ability to integrate multiple partitions different solutions improve robustness, stability, scalability parallelization algorithms. By can discover underlying classes samples in gene expression Results: In addition exploring a...
In this study, we aimed to investigate the associations between Sirt6, macrophages and diabetic nephropathy (DN). Immunohistochemical, western blot RT‑qPCR analyses were performed detect expression levels of markers podocytes monocytes related inflammatory factors in tissues rats with streptozocin‑induced DN. A series cell experiments isolated culture or co‑culture conducted examine effects overexpression Sirt6 on macrophage transformation, podocyte apoptosis associated genes, using RT‑qPCR,...
Abstract Purpose: Glioblastoma (GBM) is a heterogeneous malignancy with multiple subpopulations of cancer cells present within any tumor. We the results phase I clinical trial using an autologous dendritic cell (DC) vaccine pulsed lysate derived from GBM stem-like line. Patients and Methods: newly diagnosed recurrent were enrolled as separate cohorts. Eligibility criteria included qualifying surgical resection or minimal tumor size, ≤ 4-mg dexamethasone daily dose, Karnofsky score ≥70....
The human ether a-go-go-related gene 1 (HERG1) is involved in tumor progression; however, its role esophageal squamous cell carcinoma (ESCC) not well studied. This study investigated HERG1 function ESCC progression and elucidated the underlying mechanisms. prognostic value of was determined by immunohistochemistry biopsies. Cell growth proliferation were analyzed colony formation methyl thiazolyl tetrazolium assays. migration invasion wound healing Boyden transwell Epithelial-mesenchymal...
In order to explore the possibility of l-carnitine (LC) as a protector male fertility in chemotherapy, we observed damage cyclophosphamide (CTX) Sertoli cells and protective effect LC on testis from such this study. Healthy adult mice were divided into three groups: chemotherapy group injected intraperitoneally with CTX; agent both control only isochoric physiological saline; all once day for 5 days. After days, were, respectively, killed at 24 hr after last injection. The epididymis...
Abstract Seminal extracellular vesicles (EVs) contain different subgroups that have diverse effects on sperm function. However, the effect of seminal EVs—especially their subgroups—on endometrial receptivity is largely unknown. Here, we found EVs could be divided into high-density (EV-H), medium density EVs, and low-density after purification using iodixanol. We demonstrated EV-H promote expression secretion leukemia inhibitor factor (LIF) in human cells. In EV-H–treated cells, identified...
Abstract Glioblastoma (GBM) is the most aggressive primary brain tumor, with a five-year survival rate of only 5-7%. Current therapies include surgery, chemotherapy, and radiation, but there an urgent need for alternative strategies to reduce side effects these treatments. The paradigm cancer treatment shifting toward immunotherapy, which offers tumor target specificity through mechanisms that specific antigen receptors. Macrophages play major role in innate immune system, primarily...
INTRODUCTION: Glioblastoma (GBM) recurrence remains an unmet medical challenge, largely attributed to resilient glioma cancer stem cells (CSCs) that evade conventional therapies. TCR T bear virally infected cell receptors for targeting antigen-bearing tumor cells. NY-ESO-1-specific receptor (TCR) can target GBM Decitabine (DAC) increases NY-ESO-1 expression, specifically in CSCs, potentially enabling the eradication of this population. METHODS: expression was analyzed by immunocytochemistry...
The diversity of phenylpropanoids offers a rich inventory bioactive chemicals that can be exploited for plant improvement and human health. Recent evidence suggests glycosylation may play role in the partitioning phenylpropanoid precursors variety downstream uses. This work reports functional characterization stress-responsive glycosyltransferase, GT1-316 Populus. belongs to UGT84A subfamily glycosyltransferase family 1 is designated UGT84A17. Recombinant protein analysis showed UGT84A17...
Context The varicocele is the leading cause of male infertility and can impair sperm quality testicular function through various mechanisms. In our previous study, we found that lycopene could attenuate hypoxia-induced injury. Aims To illustrate detailed mechanism on spermatocytes. Methods effect GC-2 cells under hypoxia were detected by flow cytometry western blot assay. miR-seq was used to determine miRNA expression in rat model testes. miR-23a/b determined Key results We demonstrate...
Targeting cancer stem cells (CSCs) with immunotherapy may be an effective means to prevent recurrences in glioblastoma multiforme (GBM). It is well established that CD133 expressed the population of GBM tumor representing CSCs. This raises a possibility could serve as potential target for cytotoxic T (CTLs) cells. Two human leukocyte antigen (HLA)-A*0201-restricted epitopes, ILSAFSVYV (CD133-405) and YLQWIEFSI (CD133-753), showed strong binding HLA-A*0201 molecules. In vitro immunogenicity...
In the present work, an antibiotic-producing marine bacterium was isolated from a seawater sample collected Yuhuan, Zhejiang, China, identified and named as Bacillus amyloliquefaciens ESB-2 on basis of phenotypic characteristics 16S rRNA gene sequencing. Response surface methodology applied to optimize fermentation conditions for rapid efficient accumulation macrolactin A, pharmacologically important antibiotic. Eight were examined their significance A production using Plackett-Burman...
Aerobic glycolysis plays an important role in cancer progression. New target genes regulating aerobic must be explored to improve patient prognosis. Mitochondrial topoisomerase I (TOP1MT) deficiency suppresses glucose oxidative metabolism but enhances normal cells. Here, we examined the of TOP1MT gastric (GC) and attempted determine underlying mechanism. Using vitro vivo experiments analyzing clinicopathological characteristics patients with GC, found that expression was lower GC samples...
Impressive responses have been observed in patients with cancer treated checkpoint inhibitory anti-programmed cell death-1 (PD-1) or anti-cytotoxic T-lymphocyte-associated antigen-4 (CTLA-4) antibodies through disinhibiting the immune system. However, tumors possess complex immunosuppressive tumor microenvironment to present therapeutic obstacles and response rates inhibition remain low. One significant barrier efficacy of anti-PD1 treatment is recruitment myeloid-derived suppressor cells...
Inappropriate platelet activation is the key point of thrombogenesis. The aim present study was to investigate effects resveratrol (RESV), a compound extracted from Chinese medicinal herb Polygonum cuspidatum sieb et Zucc, on induced by adenosine diphosphate (ADP) and its possible mechanism. percentage aggregation surface P-selectin-positive platelets, activity protein kinase C (PKC) were observed with aggregometer, flow cytometry phosphorimaging system, respectively. RESV at 25, 50 100...
Abstract Asparagine synthetase (ASNS) is a gene on the long arm of chromosome 7 that copy-number amplified in majority glioblastomas. ASNS amplification associated with significantly decreased survival. Using patient-derived glioma stem cells (GSC), we showed significant metabolic alterations occur gliomas when perturbing expression ASNS, which not merely restricted to amino acid homeostasis. ASNS-high GSCs maintained slower basal profile yet readily shifted greatly increased capacity for...
Ankylosing spondylitis (AS) is a chronic inflammatory autoimmune disease, and the diagnosis treatment of AS have been limited because its pathogenesis still unclear. Pyroptosis proinflammatory type cell death that plays an important role in immune system. However, relationship between pyroptosis genes has never elucidated.GSE73754, GSE25101, GSE221786 datasets were collected from Gene Expression Omnibus (GEO) database. Differentially expressed pyroptosis-related (DE-PRGs) identified by R...