A5086697599- Knee injuries and reconstruction techniques
- Total Knee Arthroplasty Outcomes
- Osteoarthritis Treatment and Mechanisms
- Orthopaedic implants and arthroplasty
- Bone fractures and treatments
- Orthopedic Infections and Treatments
- Periodontal Regeneration and Treatments
- Tendon Structure and Treatment
- Spine and Intervertebral Disc Pathology
- Sports injuries and prevention
- Cancer, Hypoxia, and Metabolism
- Shoulder Injury and Treatment
- Mesenchymal stem cell research
- Hip and Femur Fractures
- COVID-19 and healthcare impacts
- Hip disorders and treatments
- Lower Extremity Biomechanics and Pathologies
- Orthopedic Surgery and Rehabilitation
- Bone and Joint Diseases
- Muscle Physiology and Disorders
- Cancer-related molecular mechanisms research
- Musculoskeletal pain and rehabilitation
- Nutrition and Health in Aging
- Trauma and Emergency Care Studies
- Spinal Fractures and Fixation Techniques
Istituto Ortopedico Galeazzi
2016-2025
University of Milan
2016-2025
Istituto Clinico Sant'Ambrogio
2023-2025
Istituti di Ricovero e Cura a Carattere Scientifico
2017-2024
Orthopedic Institute
2024
Orthopaedic Research
2024
Bournemouth University
2024
University Hospitals Dorset NHS Foundation Trust
2024
Vita-Salute San Raffaele University
2006-2024
University of Michigan
2014-2019
Significance Heterotopic ossification (HO) is a debilitating condition in which bone forms inappropriately within soft tissues. Two vastly different patient populations are at risk for developing HO: those with musculoskeletal trauma or severe burns and genetic mutation the morphogenetic protein receptor ACVR1 (Activin type 1 receptor). In this study, we demonstrate that both of HO share common signaling pathway through hypoxia inducible factor-1α, pharmacologic inhibition knockout can...
Cartilage lesions do not heal spontaneously and predispose to osteoarthritis. Functional cartilage tissues, engineered using autologous chondrocytes, have a therapeutic advantage over conventional cellular therapies in preclinical studies. Here, we tested whether ex vivo maturation of grafts for repair leads improved patient benefit. Using nasal chondrocytes (NCs), implantation vitro-matured NC-tissue-engineered (N-TEC) versus undifferentiated NC-cell-activated matrices (N-CAM) focal would...
Umbilical cord (UC) may represent an attractive cell source for allogeneic mesenchymal stem (MSC) therapy. The aim of this in vitro study is to investigate the chondrogenic and osteogenic potential UC-MSCs grown onto tridimensional scaffolds, identify a possible clinical relevance use cartilage bone reconstructive surgery. Chondrogenic differentiation on scaffolds was confirmed at 4 weeks by expression sox-9 type II collagen; low oxygen tension improved these markers. A similar trend...
ABSTRACT Hypoxia and inflammation are implicated in the episodic induction of heterotopic endochondral ossification (HEO); however, molecular mechanisms unknown. HIF-1α integrates cellular response to both hypoxia is a prime candidate for regulating HEO. We investigated role fibrodysplasia ossificans progressiva (FOP), most catastrophic form HEO humans. found that increases intensity duration canonical bone morphogenetic protein (BMP) signaling through Rabaptin 5 (RABEP1)-mediated retention...
Nucleus pulposus (NP) cells reside in the avascular and hypoxic microenvironment of intervertebral discs. Importantly, many activities related to survival function NP are controlled by HIF-family transcription factors. We hypothesize that adapt their niche through modulation macroautophagy/autophagy. In various cell types, hypoxia induces autophagy a HIF1A-dependent fashion; however, little is known about regulation cells. Hypoxia increases number autophagosomes as seen TEM analysis...
As the coronavirus pandemic spreads, more and people are infected with severe acute respiratory syndrome 2. The short- medium-term effects of infection have been described, but description long-term sequelae is lacking in literature.Patients healed from disease 2019 (COVID-19) February 2020 to May were considered for inclusion this study, regardless severity during phase. Eligible patients consecutively contacted a semistructured interview was administered between March 2021 by trained...
Abstract Current evidence on the quality of sports activity in patients younger than 65 following dual mobility (DM) total hip arthroplasty (THA) is lacking, and whether this coupling allows better performance single (SM) still needs to be fully clarified. This clinical trial compared sport-related patient-reported outcome measures (PROMs) traditional SM versus DM implants active 65. All THAs were performed using a minimally invasive posterolateral approach, polyethylene liner ceramic head....
The intervertebral disc (IVD) is one of the largest avascular organs in vertebrates. nucleus pulposus (NP), a highly hydrated and proteoglycan-enriched tissue, forms inner portion IVD. NP surrounded by multi-lamellar fibrocartilaginous structure, annulus fibrosus (AF). This structure covered superior inferior side cartilaginous endplates (CEP). unique tissue within IVD as it results from differentiation notochordal cells, whereas, AF CEP derive sclerotome. hypoxia inducible factor-1α...
Abstract Purpose Current conservative treatments for knee OA provide limited benefits, with symptoms relief a short amount of time. Regenerative medicine approaches such as the use microfragmented adipose tissue (mFAT) showed promising results in terms durable effects and possibility to enhance healing counteract progression pathology. Nevertheless, up today, large part clinical data about mFAT refers uncontrolled studies, especially surgical setting. The purpose this study was evaluate...
Abstract Purpose The purpose of this study was to assess the frequency medial collateral ligament (MCL), posterior oblique (POL) and anterolateral (ALL) tears different types RAMP lesions patients with verified acute anterior cruciate (ACL) by magnetic resonance imaging (MRI). Methods MRI performed on a clinical diagnosis ACL injury. Patients were eligible for inclusion if they had an initially clinically noted tear confirmed within 30 days trauma. Results A total 146 included in study, 42...
Background: This study evaluates the potential of ChatGPT and Google Bard as educational tools for patients in orthopedics, focusing on sports medicine pediatric orthopedics. The aim is to compare quality responses provided by these natural language processing (NLP) models, addressing concerns about dissemination incorrect medical information. Methods: Ten ACL- flat foot-related questions from a search were presented ChatGPT-3.5 Bard. Expert orthopedic surgeons rated using Global Quality...
Abstract Osteoblasts, which are the bone-forming cells, operate in a hypoxic environment. The transcription factors hypoxia-inducible factor-1α (HIF1) and HIF2 key mediators of cellular response to hypoxia. Both expressed osteoblasts. HIF1 is known be positive regulator bone formation. Conversely, role control osteoblast biology still poorly understood. In this study, we used mouse genetics demonstrate that an inhibitor osteoblastogenesis mass accrual. Moreover, provided evidence impairs...
Skeletal muscle regeneration is a complex process involving the generation of new myofibers after trauma, competitive physical activity, or disease. In this context, adult skeletal stem cells, also known as satellite cells (SCs), play crucial role in regulating tissue homeostasis and activating regeneration. Alterations their number function have been associated with various pathological conditions. The main factors involved dysregulation SCs' activity are inflammation, oxidative stress,...
In recent years, there has been increased interest in the management of medial femorotibial knee osteoarthritis (OA) conjunction with anterior cruciate ligament (ACL) deficiency. Traditional treatment modalities included conservative therapy, high tibial osteotomy or without ACL reconstruction, and total replacement. Since younger patients higher physical demands are more likely to suffer from this pathological condition, reduced invasiveness, faster recovery time, improved kinematics...
One of the crucial points for a successful tissue-engineering approach cartilage repair is represented by level in vitro maturation engineered tissue before implantation. The purpose this work was to evaluate effect cartilaginous samples on quality after vivo Samples were obtained from isolated swine articular chondrocytes embedded fibrin glue. cell-fibrin composites either cultured or directly implanted 1, 5, and 9 weeks. Other experimental precultured 1 5 weeks then 4 additional All...