A5008085561- Burn Injury Management and Outcomes
- Wound Healing and Treatments
- Liver physiology and pathology
- Injury Epidemiology and Prevention
- Adipose Tissue and Metabolism
- Immune Response and Inflammation
- Cardiac Arrest and Resuscitation
- Organ Transplantation Techniques and Outcomes
- Clinical Nutrition and Gastroenterology
- Trauma and Emergency Care Studies
- Pancreatic function and diabetes
- Sepsis Diagnosis and Treatment
- Microfluidic and Bio-sensing Technologies
- 3D Printing in Biomedical Research
- Disaster Response and Management
- Thermal Regulation in Medicine
- Vitamin C and Antioxidants Research
- Metabolism and Genetic Disorders
- Monoclonal and Polyclonal Antibodies Research
- Muscle metabolism and nutrition
- Mitochondrial Function and Pathology
- Tissue Engineering and Regenerative Medicine
- Sulfur Compounds in Biology
- Neonatal Respiratory Health Research
- Microfluidic and Capillary Electrophoresis Applications
Harvard University
2013-2024
Massachusetts General Hospital
2015-2024
University of Tampa
2024
New York Proton Center
2024
California State University, Fresno
2024
University of Portland
2024
Greenville College
2024
Hudson Institute
2024
University of San Diego
2024
Indianapolis Zoo
2024
A cornerstone of modern biomedical research is the use mouse models to explore basic pathophysiological mechanisms, evaluate new therapeutic approaches, and make go or no-go decisions carry drug candidates forward into clinical trials. Systematic studies evaluating how well murine mimic human inflammatory diseases are nonexistent. Here, we show that, although acute stresses from different etiologies result in highly similar genomic responses humans, corresponding correlate poorly with...
Under laminar flow conditions, when no external forces are applied, particles generally thought to follow fluid streamlines. Contrary this perspective, we observe that flowing migrate across streamlines in a continuous, predictable, and accurate manner microchannels experiencing flows. The migration is attributed lift on observed inertial aspects of the become significant. We identified symmetric asymmetric channel geometries provide additional bias particular equilibrium positions create...
The use of tyrosine kinase inhibitors to target the epidermal growth factor receptor gene (EGFR) in patients with non-small-cell lung cancer is effective but limited by emergence drug-resistance mutations. Molecular characterization circulating tumor cells may provide a strategy for noninvasive serial monitoring genotypes during treatment.We captured highly purified from blood using microfluidic device containing microposts coated antibodies against epithelial cells. We performed EGFR...
A Pseudomonas aeruginosa strain (UCBPP-PA14) is infectious both in an Arabidopsis thaliana leaf infiltration model and a mouse full-thickness skin burn model. UCBPP-PA14 exhibits ecotype specificity for , causing range of symptoms from none to severe four different ecotypes. In the model, as lethal other well-studied P. strains. Mutations toxA plcS gacA genes resulted significant reduction pathogenicity hosts, indicating that these encode virulence factors required full expression plants animals.
Human survival from injury requires an appropriate inflammatory and immune response. We describe the circulating leukocyte transcriptome after severe trauma burn injury, as well in healthy subjects receiving low-dose bacterial endotoxin, show that these stresses produce a global reprioritization affecting >80% of cellular functions pathways, truly unexpected “genomic storm.” In blunt trauma, early genomic response is consistent with simultaneously increased expression genes involved...
Over the past 20 years, there has been remarkable improvement in chances of survival patients treated burn centers. A simple, accurate system for objectively estimating probability death would be useful counseling and making medical decisions.
Interleukins (IL)-1β and -1α tumor necrosis factor (TNF-α) were measured by radioimmunoassay in plasma samples from 44 healthy individuals, 15 patients septic shock, 6 volunteers infused with endotoxin. Plasma IL-1α levels low (40 pg/ml) or undetectable all situations. In 67% ofthe subjects, IL-1β <70 pg/ml. Septic had higher (120± 17pg/ml, P =.001);those of surviving than those who died (P = .05). TNF-α concentrations individuals elevated (119 ± 30 correlated severity illness (r .73, .003),...
Adult rat hepatocytes cultured in a collagen sandwich system maintained normal morphology and physiological rate of albumin secretion for at least 42 days. Hepatocytes on single layer gel essentially ceased within 1 wk but could recover function with the overlay second gel. This culture configuration more closely mimics hepatocytes' vivo environment provides simple method their long-term maintenance.— Dunn, J. C. Y.; Yarmush, M. L.; Koebe, H. G.; Tompkins, R. G. Hepatocyte extracellular...
Abstract In an effort to reconstruct the cellular polarity normally found in liver, adult rat hepatocytes were sandwiched between two layers of hydrated tail tendon collagen matrix. Functionally, maintained secretion albumin, transferrin, fibrinogen, bile acids, and urea for at least 6 weeks, whereas cells cultured on a single layer gel ceased such 1–2 weeks. After 1 week culture gel, could still recover these lost functions when second was applied. The exact nature substrate constructing...
Because of their extensive wounds, burn patients are chronically exposed to inflammatory mediators. Thus, patients, by definition, already have “systemic response syndrome.” Current definitions for sepsis and infection many criteria (fever, tachycardia, tachypnea, leukocytosis) that routinely found in with burns, making these current less applicable the population. Experts care research, all members American Burn Association, were asked review literature prepare a potential definition on one...
Bacterial communities use "quorum sensing" (QS) to coordinate their population behavior through the action of extracellular signal molecules, such as N-acyl-l-homoserine lactones (AHLs). The versatile and ubiquitous opportunistic pathogen Pseudomonas aeruginosa is a well-studied model for AHL-mediated QS. This species also produces an intercellular distinct from AHLs, 3,4-dihydroxy-2-heptylquinoline (PQS), which belongs family poorly characterized 4-hydroxy-2-alkylquinolines (HAQs)...
We reported recently that the human opportunistic pathogen Pseudomonas aeruginosa strain PA14 kills Caenorhabditis elegans and many P. virulence factors (genes) required for maximum in mouse pathogenicity are also killing of C. elegans. Here we report among eight Tn phoA mutants isolated exhibited reduced , at least five mice. Three corresponded to known virulence-related genes lasR, gacA lemA . ( aefA from Escherichia coli, pstP Azotobacter vinelandii mtrR Neisseria gonorrhoeae ) had not...
Characterizing the genome-wide dynamic regulation of gene expression is important and will be much interest in future. However, there currently no established method for identifying differentially expressed genes a time course study. Here we propose significance analyzing microarray studies that can applied to typical types comparisons sampling schemes. This two on humans. In one study, are identified show differential over response vivo endotoxin administration. By using our method, 7,409...
Rare circulating tumor cells (CTCs) are present in the blood of patients with metastatic epithelial cancers but have been difficult to measure routinely. We report a quantitative automated imaging system for analysis prostate CTCs, taking advantage prostate-specific antigen (PSA), unique tumor-associated marker. The specificity PSA staining enabled optimization criteria baseline image intensity, morphometric measurements, and integration multiple signals three-dimensional microfluidic...
Rapid separation and filtration of particles in solution has a wide range applications including blood cell separation, ultrasound contrast agent preparation, purification fermentation products. However, current techniques that provide quick processing rates are high complexity. We present rapid microfluidic technology capable separating based on size, with purities from 90 to 100% high-volume throughputs 1 mL/min. Data for rigid particles, deformable emulsions, platelets whole presented....
Practical HIV diagnostics are urgently needed in resource-limited settings. While infection can be diagnosed using simple, rapid, lateral flow immunoassays, disease staging and treatment monitoring require accurate counting of a particular white blood cell subset, the CD4(+) T lymphocyte. To address limitations current expensive, technically demanding and/or time-consuming approaches, we have developed simple CD4 microfluidic device. This device uses affinity chromatography operated under...
Objective: Characterizing burn sizes that are associated with an increased risk of mortality and morbidity is critical because it would allow identifying patients who might derive the greatest benefit from individualized, experimental, or innovative therapies. Although scores have been established to predict mortality, few data addressing other outcomes exist. The objective this study was determine after burn. Design Patients: Burn were prospectively enrolled as part multicenter prospective...
Significance Heterotopic ossification (HO) is a debilitating condition in which bone forms inappropriately within soft tissues. Two vastly different patient populations are at risk for developing HO: those with musculoskeletal trauma or severe burns and genetic mutation the morphogenetic protein receptor ACVR1 (Activin type 1 receptor). In this study, we demonstrate that both of HO share common signaling pathway through hypoxia inducible factor-1α, pharmacologic inhibition knockout can...
The human opportunistic pathogen Pseudomonas aeruginosa strain PA14 infects both plants and animals. Previously, using to screen directly for P. virulence-attenuated mutants, we identified a locus, pho34B12 , relevant in mammalian pathogenesis. Here, nonsense point mutations the two opposing ORFs locus revealed that one of them, mvfR ( m ultiple v irulence f actor R egulator), is able control all phenotypes mutant phoA34B12 displays. Both genetic biochemical evidence demonstrate gene encodes...