A5013961501- Immunodeficiency and Autoimmune Disorders
- Bacterial biofilms and quorum sensing
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Antibiotic Resistance in Bacteria
- Vibrio bacteria research studies
- Blood disorders and treatments
- Bacterial Genetics and Biotechnology
- Electrospun Nanofibers in Biomedical Applications
- Tuberculosis Research and Epidemiology
- Immune responses and vaccinations
- Pediatric health and respiratory diseases
- Conducting polymers and applications
- Neonatal Respiratory Health Research
- Inflammasome and immune disorders
- Cerebrovascular and Carotid Artery Diseases
- Advanced Sensor and Energy Harvesting Materials
- Congenital Heart Disease Studies
- Antifungal resistance and susceptibility
- Cytokine Signaling Pathways and Interactions
- Immune Response and Inflammation
- Pediatric Hepatobiliary Diseases and Treatments
- Congenital heart defects research
- Intestinal Malrotation and Obstruction Disorders
- Plant Pathogenic Bacteria Studies
- Asthma and respiratory diseases
Zhongyuan University of Technology
2013-2024
Gansu University of Traditional Chinese Medicine
2013-2024
Beijing Children’s Hospital
2010-2023
Capital Medical University
2010-2023
National Clinical Research Center for Digestive Diseases
2019-2023
Center for Children
2023
First Affiliated Hospital of Nanchang University
2022
Nanchang University
2022
Capital University
2021
First Affiliated Hospital of Henan University
2020
Bacterial communities use "quorum sensing" (QS) to coordinate their population behavior through the action of extracellular signal molecules, such as N-acyl-l-homoserine lactones (AHLs). The versatile and ubiquitous opportunistic pathogen Pseudomonas aeruginosa is a well-studied model for AHL-mediated QS. This species also produces an intercellular distinct from AHLs, 3,4-dihydroxy-2-heptylquinoline (PQS), which belongs family poorly characterized 4-hydroxy-2-alkylquinolines (HAQs)...
Abstract Background Pseudomonas aeruginosa is a ubiquitous environmental bacterium and an important opportunistic human pathogen. Generally, the acquisition of genes in form pathogenicity islands distinguishes pathogenic isolates from nonpathogens. We therefore sequenced highly virulent strain P. , PA14, compared it with previously (and less pathogenic) strain, PAO1, to identify novel virulence genes. Results The PA14 PAO1 genomes are remarkably similar, although has slightly larger genome...
The ubiquitous bacterium Pseudomonas aeruginosa is the quintessential opportunistic pathogen. Certain isolates infect a broad range of host organisms, from plants to humans. pathogenic promiscuity particular variants may reflect an increased virulence gene repertoire beyond core P. genome. We have identified and characterized two pathogenicity islands (PAPI-1 PAPI-2) in genome PA14, highly virulent clinical isolate. 108-kb PAPI-1 11-kb PAPI-2, which are absent less reference strain PAO1,...
Etiological agents of acute, persistent, or relapsing clinical infections are often refractory to antibiotics due multidrug resistance and/or antibiotic tolerance. Pseudomonas aeruginosa is an opportunistic Gram-negative bacterial pathogen that causes recalcitrant and severe acute chronic persistent human infections. Here, we target the MvfR-regulated P. quorum sensing (QS) virulence pathway isolate robust molecules specifically inhibit infection without affecting growth viability mitigate...
Summary MvfR (PqsR), a Pseudomonas aeruginosa LysR‐type transcriptional regulator, plays critical role in the virulence of this pathogen. modulates expression multiple quorum sensing (QS)‐regulated factors; and phnAB pqsA‐E genes that encode functions mediating 4‐hydroxy‐2‐alkylquinolines (HAQs) signalling compounds biosynthesis, including 3,4‐dihydroxy‐2‐heptylquinoline (PQS) its precursor 4‐hydroxy‐2‐heptylquinoline (HHQ). PQS enhances vitro DNA‐binding affinity to promoter, suggest it...
The opportunistic pathogen Pseudomonas aeruginosa produces a large array of 4-hydroxy-2-alkylquinolines (HAQs). These compounds were analyzed by LC/MS, using positive electrospray ionization, in the culture supernatant strain PA14. Fifty-six HAQs and related detected their [M+H]+ ions further collision induced dissociation (CID). grouped into five different series based on presence an hydrogen or hydroxyl group at 3 position, N-oxide place quinoline nitrogen, unsaturation alkyl side chain....
Pseudomonas aeruginosa harbours three type VI secretion (T6S) loci. Although HSI-I has been partially studied, limited knowledge is available on the homologous loci HSI-II and HSI-III. We show that quorum sensing (QS) differentially regulates expression of genes at all HSI-I-associated gene suppressed by both homoserine lactone transcription factor LasR 4-hydroxy-2-alkylquinoline (HAQ) transcriptional regulator MvfR. Conversely, HSI-III are positively controlled PqsE, a key component MvfR...
The LysR-type transcriptional regulator MvfR (PqsR) (multiple virulence factor regulator) plays a critical role in Pseudomonas aeruginosa pathogenicity via the regulation of multiple quorum-sensing (QS)-regulated factors. LasR activates full mvfR transcription, and subsequently pqsA-E expression. This study identifies characterizes key cis-regulatory elements through which transcription is regulated highly virulent P. strain PA14. Deletion site-directed mutagenesis indicate that: (1) by...
A significant number of environmental microorganisms can cause serious, even fatal, acute and chronic infections in humans. The severity outcome each type infection depends on the expression specific bacterial phenotypes controlled by complex regulatory networks that sense respond to host environment. Although signals contribute a successful have been identified pathogens, mediate onset establishment yet be discovered. We volatile, low molecular weight molecule, 2-amino acetophenone (2-AA),...
Pathogenic bacteria use interconnected multi-layered regulatory networks, such as quorum sensing (QS) networks to sense and respond environmental cues external internal bacterial cell signals, thereby adapt exploit target hosts. Despite the many advances that have been made in understanding QS regulation, little is known regarding how these inputs are integrated processed context of networks. Here we report examination Pseudomonas aeruginosa 4-hydroxy-2-alkylquinolines (HAQs) MvfR network...
Bacteria can be refractory to antibiotics due a sub-population of dormant cells, called persisters that are highly tolerant antibiotic exposure. The low frequency and transience the “persister” trait has complicated elucidation mechanism controls tolerance. In this study, we show 2’ Amino-acetophenone (2-AA), poorly studied but diagnostically important small, volatile molecule produced by recalcitrant gram-negative human pathogen Pseudomonas aeruginosa, promotes tolerance in response...
Mutant p53 (R248Q) induces doxorubicin (ADM) resistance in hepatocellular carcinoma (HCC). Dihydroartemisinin (DHA) can synergistically enhance anticancer effect of many chemotherapeutic agents. However, whether DHA could increase therapeutic efficacy ADM (R248Q)-expressing HCC cells remains unknown. In the present study, we established mutant Hep3B to study and mechanism on synergistic with ADM. We found that P-gp was highly expressed cells. As a result, expressing displayed higher cell...
Increasing evidence indicates that bacterial quorum sensing (QS) signals are important mediators of immunomodulation. However, whether microbes utilize these immunomodulatory to maintain infection remain unclear. Here, we show the Pseudomonas aeruginosa QS-regulated molecule 2-amino acetophenone (2-AA) modulates host immune responses in a manner increases ability cope with this pathogen. Mice treated 2-AA prior had 90% survival compared 10% rate observed non-pretreated infected mice. Whilst...
A novel multi‐nozzle bubble electrospinning apparatus, including spinning unit, metering pump, constant flow metal funnel and yarn winder, was designed for the preparation of continuous twisted polyacrylonitrile nanofiber yarns, principle studied. An innovative unit consisting nozzle air chamber used to improve production nanofibers. Double conjugate developed using two pairs oppositely charged units neutralize charges. The effects applied voltage, rate, overall solution rate rotary speed on...
Matrine, a main active component extracted from dry roots of Sophora flavecens, has been reported to exert antitumor effects on A549 human non-small lung cancer cells, but its mechanisms action remain unclear. To determine matrine proliferation cells and assess possible mechanisms, MTT assays were employed detect cytotoxicity, along with o flow cytometric analysis DNA content nuclei following staining propidium iodide analyze cell cycle distribution. Western blotting was performed determined...
Chronic granulomatous disease (CGD) is a rare in China, and very little large-scale studies have been conducted to date. We aimed investigate the clinical genetic features of CGD Chinese pediatric patients.Pediatric patients with from Beijing Children's Hospital, Capital Medical University, were enrolled January 2006 December 2016.A total 159 enrolled. The median age onset was 1.4 months, 73% (116/159) had symptoms before 1 year age. most common site invasion lungs. lymph nodes, liver, skin...
Enteral feed is an important component of nutritional therapy in critically ill patients and underfeeding has been associated with adverse outcomes. The article developed enteral feeding protocol planed a before-and-after comparative trial to explore whether implementation was able improve clinical outcomes.The study will be conducted intensive care units (ICUs) ten tertiary academic centers. Critically expected stay ICU for over 3 days require nutrition (EN) were potentially eligible. This...
The development of multifunctional nanoplatform, which integrates biosensing and imaging diagnosis, for accurate identification cancer cells in blood or other bodily fluids is critical early diagnosis treatment. Herein, by using a facile single spinneret electrospinning technique followed thermal treatment, CuO-NiO/C heterojunction nanofiber was synthesized. resultant nanofibers exhibited excellent catalytic activity toward ammonia borane (AB) hydrolysis, producing marked pressure increase...
The solute carrier (SLC) 7 family genes play central roles in cancer cell metabolism as glucose and glutamate transporters. However, their expression prognostic value breast (BC) remains to be elucidated.Clinical data from BC patients were downloaded Cancer Genome Atlas (TCGA) the Kaplan-Meier (KM) plotter database. mechanisms underlying association between SLC7A overall survival (OS) explored using Cox regression log-rank tests. ESTIMATE gives a measure of immune-cell infiltrates....