A5038698413- Legume Nitrogen Fixing Symbiosis
- Plant Pathogenic Bacteria Studies
- Agronomic Practices and Intercropping Systems
- Plant-Microbe Interactions and Immunity
- Nematode management and characterization studies
- Economic and Technological Developments in Russia
- Bacterial biofilms and quorum sensing
- Plant nutrient uptake and metabolism
- Coastal wetland ecosystem dynamics
- Bacterial Genetics and Biotechnology
- Antibiotic Resistance in Bacteria
- Toxin Mechanisms and Immunotoxins
- Plant Virus Research Studies
- Genomics and Phylogenetic Studies
- Plant pathogens and resistance mechanisms
- Agricultural pest management studies
- Plant Disease Management Techniques
- Cell Image Analysis Techniques
- Plant tissue culture and regeneration
- Composting and Vermicomposting Techniques
- Transgenic Plants and Applications
- Bacillus and Francisella bacterial research
- Crop Yield and Soil Fertility
- Agricultural Science and Fertilization
- Single-cell and spatial transcriptomics
Hellenic Mediterranean University
2004-2024
Agricultural University of Athens
2009-2021
Burn Institute
2006
Harvard University
2005-2006
Massachusetts General Hospital
2005-2006
University of Crete
2003-2004
FORTH Institute of Molecular Biology and Biotechnology
2000-2004
Foundation for Research and Technology Hellas
2001-2003
University of Leeds
2003
de Duve Institute
2000
Summary The transcriptional regulator MvfR is required for full Pseudomonas aeruginosa virulence, the function of multiple quorum sensing (QS)‐regulated virulence factors and synthesis 4‐hydroxy‐2‐alkylquinolines (HAQs), including quinolone signal (PQS). Here we investigate role in QS circuitry P. pathogenesis. We demonstrate using a combination biochemical molecular approaches, transcription profiling, that involved regulation QS‐controlled genes without altering expression lasRI / rhlRI or...
Summary MvfR (PqsR), a Pseudomonas aeruginosa LysR‐type transcriptional regulator, plays critical role in the virulence of this pathogen. modulates expression multiple quorum sensing (QS)‐regulated factors; and phnAB pqsA‐E genes that encode functions mediating 4‐hydroxy‐2‐alkylquinolines (HAQs) signalling compounds biosynthesis, including 3,4‐dihydroxy‐2‐heptylquinoline (PQS) its precursor 4‐hydroxy‐2‐heptylquinoline (HHQ). PQS enhances vitro DNA‐binding affinity to promoter, suggest it...
Bacteria currently included in Rhizobium leguminosarum are too diverse to be considered a single species, so we can refer this as species complex (the Rlc). We have found 429 publicly available genome sequences that fall within the Rlc and these show is distinct entity, well separated from other genus. Its sister taxon R. anhuiense. constructed phylogeny based on concatenated of 120 universal (core) genes, calculated pairwise average nucleotide identity (ANI) between all genomes. From...
The hrp gene clusters of plant pathogenic bacteria control pathogenicity on their host plants and ability to elicit the hypersensitive reaction in resistant plants. Some products constitute elements type III secretion system, by which effector proteins are exported delivered into cells. Here, we show that hrpZ product from bean halo-blight pathogen, Pseudomonas syringae pv. phaseolicola (HrpZ(Psph)), is secreted an hrp-dependent manner P. system mammalian pathogen Yersinia enterocolitica....
In the current study, inoculation with plant growth-promoting rhizobacteria (PGPR) and grafting were tested as possible cultural practices that may enhance resilience of tomato to stress induced by combined water nutrient shortage. The roots grown on perlite either inoculated or not PGPR, applying four different treatments. These PGPR-T1, a mix two Enterobacter sp. strains (C1.2 C1.5); PGPR-T2, Paenibacillus strain DN1.2; PGPR-T3, mori C3.1; PGPR-T4, Lelliottia D2.4. PGPR-treated plants...
Time-lapse microscopy is an essential tool for capturing and correlating bacterial morphology gene expression dynamics at single-cell resolution. However state-of-the-art computational methods are limited in terms of the complexity cell movies that they can analyze lack automation. The proposed Bacterial image analysis driven Single Cell Analytics (BaSCA) pipeline addresses these limitations thus enabling high throughput systems microbiology. BaSCA segment track multiple colonies...
Type III secretion systems enable plant and animal bacterial pathogens to deliver virulence proteins into the cytosol of eukaryotic host cells, causing a broad spectrum diseases including bacteremia, septicemia, typhoid fever, bubonic plague in mammals, localized lesions, systemic wilting, blights plants. In addition, type are also required for biogenesis flagellum. The HrcQ B protein, component apparatus Pseudomonas syringae with homologues all systems, has variable N-terminal conserved...
To explore possible sources of transgenic resistance to the rhizomania-causing Beet necrotic yellow vein virus (BNYVV), Nicotiana benthamiana plants were constructed express harpin Pseudomonas syringae pv. phaseolicola (HrpZ(Psph)). The HrpZ protein was expressed as an N-terminal fusion PR1 signal peptide (SP/HrpZ) direct accumulation plant apoplast. Transgene integration verified by mPCR in all primary transformants (T0), while immunoblot analysis confirmed that HrpZ(Psph) produced and...
NopT1 and NopT2, putative type III effectors from the plant symbiotic bacterium Bradyrhizobium japonicum, are predicted to belong a family of YopT/AvrPphB effectors, which cysteine proteases. In present study, we showed that both NopT2 indeed possess protease activity. When overexpressed in Escherichia coli, undergo autoproteolytic processing is largely abolished presence E-64, papain family-specific inhibitor. Mutations disrupting either catalytic triad or site reduce markedly abolish...
We present a digital twin framework for simulating microbial communities at the single-cell level, integrating genetic mechanisms through individual-based modeling (IBM). This in silico approach enables study of bacterial populations with bio-sensing capabilities and stochastic virulence expression, facilitating design biotechnological applications such as targeted drug delivery. By combining kinetic IBM, we capture regulatory interplay between quorum sensing (QS) virulence, allowing...
The ability of the Pseudomonas syringae pv. phaseolicola harpin (HrpZPsph) to elicit hypersensitive response was investigated in three Nicotiana genotypes. hrpZPsph gene placed under chemical regulation (tetracycline induction) TetR+ tabacum cv. Wisconsin 38 (W38) or transiently expressed N. benthamiana following infection with a PVX-derived vector and genotypes by agroinfiltration. constructs were designed express either canonical form an N-terminally extended version protein carrying...
The hrp gene clusters of plant pathogenic bacteria control pathogenicity on their host plants and ability to elicit the hypersensitive reaction in resistant plants. Some products constitute elements type III secretion system, by which effector proteins are exported delivered into cells. Here, we show that hrpZ product from bean halo-blight pathogen, Pseudomonas syringae pv. phaseolicola (HrpZ Psph ), is secreted an -dependent manner P. system mammalian pathogen Yersinia enterocolitica . HrpZ...
Pseudomonas fluorescens strain X, a bacterial isolate from the rhizosphere of bean seedlings, has ability to suppress damping-off caused by oomycete Pythium ultimum. To determine genes controlling biocontrol activity transposon mutagenesis, sequencing and complementation was performed. Results indicate that, this is attributed gcd gene encoding glucose dehydrogenase, its co-enzyme pyrroloquinoline quinone (PQQ), two (sup5 sup6) which seem be organized in putative operon. This operon (named...
Phaseolus vulgaris (L.), commonly known as bean or common bean, is considered a promiscuous legume host since it forms nodules with diverse rhizobial species and symbiovars. Most of the nodulating rhizobia are mainly affiliated to genus Rhizobium, though strains belonging Ensifer, Pararhizobium, Mesorhizobium, Bradyrhizobium, Burkholderia have also been reported. This first report on characterization bean-nodulating at symbiovar level in Greece. The goals this research were isolate...
Information about the availability of soil mineral nitrogen (N) in organic greenhouse tomatoes after application mobile green manure (MGM), and its impact on plant nutrient status yield is scarce. Considering this knowledge gap, effects legume biomass from faba beans that are cultivated outdoors (FAB), or feed-grade alfalfa pellets at two different doses (AAL = 330 g m-2; AAH 660 m-2) were applied as MGM nutrition an crop evaluated. All treatments increased N concentrations throughout...
Global agricultural systems are increasingly moving towards organic farming to satisfy consumers' increased environmental awareness. Yet, shortage of fertilizers and more frequent water stresses challenging minimize their impact without compromising productivity economic sustainability. This study discusses how greenhouse tomato production behaves when multiple eco-sustainable solutions applied. In particular, cultivation was supported by a specific biostimulant treatment that included...
The presence of nitrogen fixers within the genus Pseudomonas has been established and so far most isolated strains are phylogenetically affiliated to stutzeri. A gene ortholog neighborhood analysis fixation island (NFI) in four diazotrophic P. stutzeri azotifigens revealed that all flanked by genes coding for cobalamin synthase (cobS) glutathione peroxidise (gshP). putative NFIs lack features characterizing a mobilizable genomic island. Nevertheless, bioinformatic DSM 4166 NFI demonstrated...
In the present study, in addition to farmyard manure (FYM), cowpea was applied as green and faba bean an intercrop organic greenhouse tomato crop, aiming increase levels of soil N. Three experiments (E1, E2, E3) were carried out, which legumes either noninoculated or inoculated with rhizobia alone together plant growth, promoting rhizobacteria. Inoculation considerably increased N2 fixation E1 but had no impact on E2 E3. E1, application decreased yield because it imposed a stronger nematode...
Abstract BACKGROUND Cowpea ( Vigna unguiculata ) forms nitrogen‐fixing root nodules with diverse symbiotic bacteria, mainly slow‐growing rhizobial species belonging to the genus Bradyrhizobium , although a few studies have reported isolation of fast‐growing rhizobia under laboratory and field conditions. Although much research has been done on cowpea‐nodulating bacteria in various countries around world, very limited information is available cowpea European soils. The aim this study was...