A5087323774- Microfluidic and Bio-sensing Technologies
- Microfluidic and Capillary Electrophoresis Applications
- 3D Printing in Biomedical Research
- Innovative Microfluidic and Catalytic Techniques Innovation
- Biosensors and Analytical Detection
- Cellular Mechanics and Interactions
- Electrowetting and Microfluidic Technologies
- Cell Image Analysis Techniques
- Cancer Cells and Metastasis
- Single-cell and spatial transcriptomics
- Advanced Biosensing Techniques and Applications
- Electrohydrodynamics and Fluid Dynamics
- Digital Holography and Microscopy
- Particle Dynamics in Fluid Flows
- Micro and Nano Robotics
- Advanced biosensing and bioanalysis techniques
- Electrospun Nanofibers in Biomedical Applications
- SARS-CoV-2 detection and testing
- Orbital Angular Momentum in Optics
- Advanced Fluorescence Microscopy Techniques
- Mosquito-borne diseases and control
- Cancer Genomics and Diagnostics
- CAR-T cell therapy research
- Nanopore and Nanochannel Transport Studies
- Lattice Boltzmann Simulation Studies
University of California, Los Angeles
2016-2025
California NanoSystems Institute
2016-2025
University of California System
2012-2025
Samueli Institute
2010-2024
Fluxion Biosciences (United States)
2023-2024
UCLA Jonsson Comprehensive Cancer Center
2014-2023
University of Colorado Denver
2016-2023
University of Colorado Cancer Center
2016-2023
La Jolla Bioengineering Institute
2016-2022
The University of Tokyo
2018-2022
Under laminar flow conditions, when no external forces are applied, particles generally thought to follow fluid streamlines. Contrary this perspective, we observe that flowing migrate across streamlines in a continuous, predictable, and accurate manner microchannels experiencing flows. The migration is attributed lift on observed inertial aspects of the become significant. We identified symmetric asymmetric channel geometries provide additional bias particular equilibrium positions create...
Cell state is often assayed through measurement of biochemical and biophysical markers. Although markers have been widely used, intrinsic markers, such as the ability to mechanically deform under a load, are advantageous in that they do not require costly labeling or sample preparation. However, current techniques assay cell mechanical properties had limited adoption clinical biology research applications. Here, we demonstrate an automated microfluidic technology capable probing single-cell...
It is important to quantify the distribution of behavior amongst a population individual cells reach more complete quantitative understanding cellular processes. Improved high-throughput analysis single cell requires uniform conditions for with controllable cell–cell interactions, including diffusible and contact elements. Uniform arrays static culture adherent have previously been constructed using protein micropatterning techniques but lack ability control secretions. Here we present...
The ability to detect and isolate rare target cells from heterogeneous samples is in high demand cell biology research, immunology, tissue engineering medicine. Techniques allowing label-free enrichment or detection are especially important reduce the complexity costs towards clinical applications. Single-cell deformability has recently been recognized as a unique biomarker for phenotype with implications assessment of cancer invasiveness. Using combination fluid dynamic effects microfluidic...
Nonlinearity in finite-Reynolds-number flow results particle migration transverse to fluid streamlines, producing the well-known ``tubular pinch effect'' cylindrical pipes. Here we investigate these nonlinear effects highly confined systems where size approaches channel dimensions. Experimental and numerical reveal distinctive dynamics, including complex scaling of lift forces with geometry. The unique behavior described this Letter has broad implications for particulate flows.
Encapsulation of cells within picolitre-size monodisperse drops provides new means to perform quantitative biological studies on a single-cell basis for large cell populations. Variability in the number per drop due stochastic loading is major barrier these techniques. We overcome this limitation by evenly spacing as they travel high aspect-ratio microchannel; enter generator with frequency formation.
A blood-based, low cost alternative to radiation intensive CT and PET imaging is critically needed for cancer prognosis management of its treatment. "Liquid biopsies" circulating tumor cells (CTCs) from a relatively non-invasive blood draw are particularly ideal, as they can be repeated regularly provide up date molecular information about the cancer, which would also open key opportunities personalized therapies. Beyond solely diagnostic applications, CTCs subject interest drug development...
High-quality single-cell data are required for a quantitative systems biology description of cellular function. However, this type difficult and time-consuming to collect using traditional techniques. We present robust simple microfluidic method trapping single cells in large arrays address problem. Ordered isolation allow high-density microscopic analysis with simplified image processing. Moreover, fluorescent assays, on-chip sample preparation (e.g., labeling, washing) can be performed, as...
The Lewis acid–base adduct approach has been widely used to form uniform perovskite films, which provided a methodological base for the development of high-performance solar cells. However, its incompatibility with formamidinium (FA)-based perovskites impeded further enhancement photovoltaic performance and stability. Here, we report an efficient reproducible method fabricate highly FAPbI3 films via approach. Replacement typical dimethyl sulfoxide (DMSO) N-methyl-2-pyrrolidone (NMP) enabled...
Rapid separation and filtration of particles in solution has a wide range applications including blood cell separation, ultrasound contrast agent preparation, purification fermentation products. However, current techniques that provide quick processing rates are high complexity. We present rapid microfluidic technology capable separating based on size, with purities from 90 to 100% high-volume throughputs 1 mL/min. Data for rigid particles, deformable emulsions, platelets whole presented....
ADVERTISEMENT RETURN TO ISSUEPREVFEATURENEXTDynamic Single-Cell Analysis for Quantitative BiologyDino Di Carlo and Luke P. LeeCite this: Anal. Chem. 2006, 78, 23, 7918–7925Publication Date (Web):December 1, 2006Publication History Published online1 December 2006Published inissue 1 2006https://pubs.acs.org/doi/10.1021/ac069490phttps://doi.org/10.1021/ac069490pnewsACS Publications. This publication is available under these Terms of Use. Request reuse permissions free to access through this...
Optical microscopy is one of the most widely used diagnostic methods in scientific, industrial, and biomedical applications. However, while useful for detailed examination a small number (< 10,000) microscopic entities, conventional optical incapable statistically relevant screening large populations (> 100,000,000) with high precision due to its low throughput limited digital memory size. We present an automated flow-through single-particle microscope that overcomes this limitation by...
Rapid and accurate differentiation of cell types within a heterogeneous solution is challenging but important task for various applications in biological research medicine. Flow cytometry the gold standard analysis regularly used blood (i.e., complete counts). cytometry, however, lacks sufficient throughput to analyze rare cells or other dilute solutions reasonable time period because it an inherently serial process. In this study, we exploit inertial effects label- sheath-free parallel flow...
Standard microplate based enzyme-linked immunosorbent assays (ELISA) are widely utilized for various nanomedicine, molecular sensing, and disease screening applications, this multiwell plate batched analysis dramatically reduces diagnosis costs per patient compared to nonbatched or nonstandard tests. However, their use in resource-limited field-settings is inhibited by the necessity relatively large expensive readout instruments. To mitigate problem, we created a hand-held cost-effective...
Cell separation is broadly useful for applications in clinical diagnostics, biological research, and potentially regenerative medicine. Recent attention has been paid to label-free size-based techniques that may avoid the costs or clogging issues associated with centrifugation mechanical filtration. We present first time a massively parallel microfluidic device passively separates pathogenic bacteria cells from diluted blood macroscale performance. The was designed process large sample...
Cell isolation in designated regions or from heterogeneous samples is often required for many microfluidic cell-based assays. However, current techniques have either limited throughput are incapable of viable off-chip collection. We present an innovative approach, allowing high-throughput and label-free cell enrichment solution using size as a biomarker. The approach utilizes the irreversible migration particles into microscale vortices, developed parallel expansion-contraction trapping...
The standard centrifuge is a laboratory instrument widely used by biologists and medical technicians for preparing cell samples. Efforts to automate the operations of concentration, separation, solution exchange that performs in simpler smaller platform have had limited success. Here, we present microfluidic chip replicates functions without moving parts or external forces. device operates using purely fluid dynamic phenomenon which cells selectively enter are maintained microscale vortices....