A5071584717- Photoacoustic and Ultrasonic Imaging
- Spectroscopy Techniques in Biomedical and Chemical Research
- Optical Coherence Tomography Applications
- Random lasers and scattering media
- Fatigue and fracture mechanics
- Advanced Fluorescence Microscopy Techniques
- Chromosomal and Genetic Variations
- Epigenetics and DNA Methylation
- Sports Performance and Training
- High-Velocity Impact and Material Behavior
- Cell Image Analysis Techniques
- Birth, Development, and Health
- Genomics and Phylogenetic Studies
- Spectroscopy and Chemometric Analyses
- Thermography and Photoacoustic Techniques
- RNA modifications and cancer
- Non-Destructive Testing Techniques
- Plasma Applications and Diagnostics
- Plasma Diagnostics and Applications
- High voltage insulation and dielectric phenomena
- Optical Imaging and Spectroscopy Techniques
- Hydrogen embrittlement and corrosion behaviors in metals
- Sports injuries and prevention
- Fusion materials and technologies
- Microstructure and Mechanical Properties of Steels
The University of Tokyo
2015-2024
Shizuoka Institute of Science and Technology
2024
Minamiaoyama Eye Clinic
2023
Osaka Metropolitan University
2022
Kyushu Institute of Technology
2021
Gifu Prefectural Tajimi Hospital
2021
Tokyo University of Information Sciences
2016-2020
Soka Municipal Hospital
2018
Hokkaido University
2018
Washington University in St. Louis
2011-2017
Combining the strength of flow cytometry with fluorescence imaging and digital image analysis, is a powerful tool in diverse fields including cancer biology, immunology, drug discovery, microbiology, metabolic engineering. It enables measurements statistical analyses chemical, structural, morphological phenotypes numerous living cells to provide systematic insights into biological processes. However, its utility constrained by requirement fluorescent labeling for phenotyping. Here we present...
The advent of image-activated cell sorting and imaging-based picking has advanced our knowledge exploitation biological systems in the last decade. Unfortunately, they generally rely on fluorescent labeling for cellular phenotyping, an indirect measure molecular landscape cell, which critical limitations. Here we demonstrate Raman by directly probing chemically specific intracellular vibrations via ultrafast multicolor stimulated scattering (SRS) microscopy phenotyping. Specifically,...
Photoacoustic microscopy (PAM) is uniquely positioned for biomedical applications because of its ability to visualize optical absorption contrast in vivo three dimensions. Here we propose motionless volumetric spatially invariant resolution photoacoustic (SIR-PAM). To realize imaging, SIR-PAM combines two-dimensional Fourier-spectrum excitation with single-element depth-resolved detection. achieve lateral resolution, propagation-invariant sinusoidal fringes are generated by a digital...
Centromeres and large-scale structural variants evolve contribute to genome diversity during vertebrate speciation. Here, we perform de novo long-read assembly of three inbred medaka strains that are derived from geographically isolated subpopulations undergo Using single-molecule real-time (SMRT) sequencing, obtain chromosome-mapped genomes length ~734, ~678, ~744Mbp with a resource twenty-two centromeric regions 20-345kbp. positionally conserved among the even between four pairs...
Phase distortions due to scattering in random media restrict optical focusing beyond one transport mean free path. However, can be compensated for by applying a correction the illumination wavefront using spatial light modulators. One method of obtaining is iterative determination an optimization algorithm. In past, feedback signal required either direct access target region, or invasive embedding molecular probes within media. Here, we propose ultrasonically encoded as guide dynamically and...
Abstract Dominance heritability in complex traits has received increasing recognition. However, most polygenic score (PGS) approaches do not incorporate non-additive effects. Here, we present GenoBoost, a flexible PGS modeling framework capable of considering both additive and effects, specifically focusing on genetic dominance. Building statistical boosting theory, derive provably optimal GenoBoost scores provide its efficient implementation for analyzing large-scale cohorts. We benchmark...
Long-read analysis of 36 individual genomes revealed extensive structural diversity within human centromeric repeat arrays.
Amyotrophic lateral sclerosis (ALS) is a devastating, incurable neurodegenerative disease. A subset of ALS patients manifests with early-onset and complex clinical phenotypes. We aimed to elucidate the genetic basis these cases enhance our understanding disease etiology facilitate development targeted therapies.
Scattering dominates light propagation in biological tissue, and therefore restricts both resolution penetration depth optical imaging within thick tissue. As photons travel into the diffusive regime-typically 1 mm beneath human skin, their trajectories transition from ballistic to due increased number of scattering events, which makes it impossible focus, much less track, photon paths. Consequently, methods that rely on controlled illumination are ineffective deep This problem has recently...
Abstract A chemically-activatable alkynyl steroid analogue probe has been synthesized for visualizing the lipid raft membrane domains by Raman microscopy. The probe, in which ring of its backbone is replaced with an group, was designed to enable activation alkyne signal through Eschenmoser-Tanabe fragmentation reaction oxidized cholesterol precursor bilayer membranes. observed form liquid-ordered raft-like on a model giant-liposome system similar manner as cholesterol, and large accumulated...
We present a new method for high speed tracking of fluorescence time series from single proteins. The uses fast sample flow and modified confocal microscopy, line microscopy achieves the resolution less than 20 μs. obtained B domain protein A labeled with donor acceptor fluorophores suggest conformational heterogeneity dynamic fluctuations in unfolded state.
Determining the methylation state of regions with high copy numbers is challenging for second-generation sequencing, because read length insufficient to map reads uniquely, especially when repetitive are long and nearly identical each other. Single-molecule real-time (SMRT) sequencing a promising method observing such regions, it not vulnerable GC bias, produces lengths, its kinetic information sensitive DNA modifications.
This study sought to identify biomechanical factors that determine fast and skilful execution of the seoi-nage (shoulder throw) technique by comparing kinematics between elite college judo athletes. Three-dimensional motion data were captured using a VICON-MX system with 18 cameras operating at 250 Hz as three male seven athletes performed seoi-nage. No significant difference was found in phase time turning two groups, indicating is not necessarily factor contributing quickness The maximum...
A skin mucus lectin exhibiting a homodimeric structure and an S–S bond between subunits of ∼40 kDa was purified from flathead Platycephalus indicus (Scorpaeniformes). This lectin, named FHL (FlatHead Lectin), exhibited mannose-specific activity in Ca2+-dependent manner. Although showed no homology to any previously reported lectins, it did exhibit ∼20% identity discovered plasma kallikreins coagulation factor XIs mammals Xenopus laevis. These known proteins are serine proteases play pivotal...
Time-reversed ultrasonically encoded (TRUE) optical focusing in turbid media was previously implemented using both analog and digital phase conjugation. The approach, addition to its large energy gain, can improve the focal intensity resolution by iterative focusing. However, performing at each position be time-consuming. Here, we show that gradually moving position, TRUE is improved, as a fixed continuously scanned image fluorescent targets shorter time. In addition, our setup is, best of...
Abstract Background The nutritional status during early life can have enduring effects on an animal’s metabolism, although the mechanisms underlying these long-term are still unclear. Epigenetic modifications considered a prime candidate mechanism for encoding early-life memories this critical developmental period. However, extent to which epigenetic changes occur and persist over time remains uncertain, in part due challenges associated with directly stimulating fetus specific nutrients...
Time-reversed ultrasonically encoded (TRUE) optical focusing achieves light into scattering media beyond one transport mean free path, which is desirable in biomedical optics. However, the focused energy needs to be increased for broad applications. Here, we report use of a photorefractive polymer (PRP) as phase conjugate mirror TRUE focusing. The PRP boosted by ∼ 40 times comparison previously used Bi12SiO20 crystal. As result, successfully imaged absorbing objects embedded middle plane...