A5039307294- RNA Interference and Gene Delivery
- Innovative Microfluidic and Catalytic Techniques Innovation
- Advanced biosensing and bioanalysis techniques
- Wound Healing and Treatments
- Microfluidic and Bio-sensing Technologies
- Dermatology and Skin Diseases
- Dupuytren's Contracture and Treatments
- Electrospun Nanofibers in Biomedical Applications
- Connective tissue disorders research
- Pancreatic function and diabetes
- 3D Printing in Biomedical Research
- Soft tissue tumor case studies
- Hair Growth and Disorders
- Tissue Engineering and Regenerative Medicine
- MicroRNA in disease regulation
- Immunotherapy and Immune Responses
- Algal biology and biofuel production
- Nanopore and Nanochannel Transport Studies
- Immune cells in cancer
- Digital Imaging in Medicine
- Planarian Biology and Electrostimulation
- Surgical Simulation and Training
- Sinusitis and nasal conditions
- Graphene and Nanomaterials Applications
- Hydrogels: synthesis, properties, applications
University of California, Los Angeles
2018-2024
WinnMed
2023-2024
Mayo Clinic
2024
VA Greater Los Angeles Healthcare System
2024
Neurological Surgery
2023
Saint Vincent Health System
2023
Cleveland Clinic
2023
Mayo Clinic in Florida
2023
Jacksonville College
2023
Johns Hopkins University
2014-2016
Abstract Delivery to the proper tissue compartment is a major obstacle hampering potential of cellular therapeutics for medical conditions. cells within biomaterials may improve localization, but traditional and newer void‐forming hydrogels must be made in advance with being added into scaffold during manufacturing process. Injectable, situ cross‐linking microporous scaffolds are recently developed that demonstrate remarkable ability provide matrix proliferation growth vitro three...
Abstract Intramyocardial injection of hydrogels offers great potential for treating myocardial infarction (MI) in a minimally invasive manner. However, traditional bulk generally lack microporous structures to support rapid tissue ingrowth and biochemical signals prevent fibrotic remodeling toward heart failure. To address such challenges, novel drug‐releasing annealed particle (drugMAP) system is developed by encapsulating hydrophobic drug‐loaded nanoparticles into microgel building blocks...
Techniques to analyze and sort single cells based on functional outputs, such as secreted products, have the potential transform our understanding of cellular biology well accelerate development next-generation cell antibody therapies. However, molecules rapidly diffuse away from cells, analysis these products requires specialized equipment expertise compartmentalize individual capture their secretions. Herein, we describe methods fabricate hydrogel-based chemically functionalized...
Despite successful demonstration of linear polyethyleneimine ( l PEI) as an effective carrier for a wide range gene medicine, including DNA plasmids, small interfering RNAs, mRNAs, etc., and continuous improvement the physical properties biological performance polyelectrolyte complex nanoparticles prepared from PEI nucleic acids, there still exist major challenges to produce these nanocomplexes in scalable manner, particularly PEI/DNA nanoparticles. This has significantly hindered progress...
The scale of biological discovery is driven by the vessels in which we can perform assays and analyze results, from multi-well plates to microfluidic compartments. We report on compatibility sub-nanoliter single-cell containers or "nanovials" with commercial fluorescence activated cell sorters (FACS). This recent lab a particle approach utilizes 3D structured microparticles isolate cells at existing equipment. Use flow cytometry led detection fluorescently labeled protein dynamic ranges...
Nanoparticle-mediated gene delivery is a promising alternative to viral methods; however, its use in vivo, particularly following systemic injection, has suffered from poor efficiency. Although PEGylation of nanoparticles been successfully demonstrated as strategy enhance colloidal stability, success improving efficiency limited, largely due reduced cell binding and uptake, leading transfection Here we identified an optimized scheme for DNA micellar that delivers balanced stability activity....
Production of high-energy lipids by microalgae may provide a sustainable energy source that can help tackle climate change. However, engineered to produce more usually grow slowly, leading reduced overall yields. Unfortunately, culture vessels used select cells based on growth while maintaining high biomass production, such as well plates, water-in-oil droplet emulsions, and nanowell arrays, do not production-relevant environments experience in scaled-up cultures (e.g., bioreactors or...
The CRISPR-based technology has revolutionized genome editing in recent years. This technique allows for gene knockout and evaluation of function cell lines a manner that is far easier more accessible than anything previously available. Unfortunately, the ability to extend these studies vivo syngeneic murine line implantation limited by an immune response against cells transduced stably express Cas9. In this study, we demonstrate non-integrating lentiviral vector approach can overcome...
New vaccine platforms that activate humoral immunity and generate neutralizing antibodies are required to combat emerging pathogens, including influenza virus. A slurry of antigen-loaded hydrogel microparticles anneal form a porous scaffold with high surface area for antigen uptake by infiltrating immune cells as the biomaterial degrades is demonstrated enhance immunity. Antigen-loaded-microgels elicited robust cellular response, increased CD4
In this letter, we present a machine-learning-based solution to classify wounds and normal skin based on dielectric spectroscopy approach. Using commercial network analyzer, have measured the constant of different types from multiple living mice across frequency range 10 MHz 20 GHz. The acquired data wide is processed by Data Dimensionality Reduction technique extract optimum for wound spectroscopy. results our analysis reveal that can be distinguished acquiring constants in 1 2 This finding...
To examine the epidemiology, subtypes, trends over time, and predictive factors for recurrence malignant transformation of sinonasal papillomas.A retrospective chart review 118 patients with papillomas from 2009 to 2019 was conducted at University California, Los Angeles. This study is a follow-up previously published 2000 same academic center.The mean age presentation 58.5 years, 2:1 male female ratio, average 30.1 months. The rate after complete resection 19% an 32.6 months recurrence....
Classic Ehlers-Danlos syndrome (cEDS) is a genetic disorder of the connective tissue that characterized by mutations in genes coding type V collagen. Wound healing defects are characteristic cEDS and no therapeutic strategies exist. Herein we describe murine model phenocopies wound seen humans. Our features mice with conditional loss
Insulin resistance is the major risk factor for Type 2 diabetes (T2D). In vulnerable individuals, insulin induces a progressive loss of secretion with islet pathology revealing partial deficit beta cells and amyloid derived from polypeptide (IAPP). IAPP co-expressed secreted by cells, expression both proteins being upregulated in response to resistance. If exceeds threshold clearance misfolded proteins, cell failure occurs exacerbated action toxicity compromise autophagy lysosomal pathway....
Abstract Techniques to analyze and sort single cells based on functional outputs, such as secreted products, have the potential transform our understanding of cellular biology, well accelerate development next generation cell antibody therapies. However, molecules rapidly diffuse away from cells, analysis these products requires specialized equipment expertise compartmentalize individual capture their secretions. Herein we demonstrate use suspendable microcontainers viable at high-throughput...
Abstract Biomaterial scaffolds represent a promising approach for material-based tissue regeneration. We previously developed microporous annealed particle (MAP) hydrogels - flowable, microparticle-based hydrogel in which neighboring particles are linked situ to form porous scaffold that accelerates wound healing. To promote more extensive ingrowth before degradation, we aimed slow degradation by switching the chirality of crosslinking peptides from L-peptides D-peptides. Unexpectedly,...
Carotid body tumors (CBTs) are rare neoplasms of the neuroectoderm accounting for 0.6% head and neck tumors, with a 2%-12.5% risk malignancy. While surgical resection has been associated high rate neurologic vascular complications, it remains mainstay treatment malignant CBTs. We present case 40-year-old female 5-year history progressively enlarging right-sided mass, MRI MRA showing Shamblin grade III CBT encasement internal carotid artery (ICA). Blood flow was absent in petrous segment ICA,...
In article number 2004307, Randall Lee, Dino Di Carlo, Song Li, and co-workers report a novel multi-modal drug-releasing microstructured hydrogel (drugMAP) system that is created in microfluidic device. The drugMAP loaded with nanoparticles release cocktail containing cAMP activator TGF-β inhibitor, which significantly enhances left ventricular function infarcted hearts.
Abstract Production of high-energy lipids by microalgae may provide a sustainable, renewable energy source that can help tackle climate change. However, engineered to produce more usually grow slowly, leading reduced overall yields. Unfortunately, tools enable the selection cells based on growth while maintaining high biomass production, such as well-plates, water-in-oil droplet emulsions, and nanowell arrays do not production-relevant environments experience in scaled-up cultures (e.g....
Anterior approaches to the cervical spine are among most common exposures by which neurosurgeons and orthopedic surgeons access prevertebral space ultimately disk vertebral bodies. There is a paucity of literature describing microanatomic fascial planes neck with respect anterior approaches.To delineate connections pertinent exposure.Using cadaveric model, original illustrations, Visible Human Project, an surgical video, we demonstrate stepwise method for identifying correct exposure.A...
Glucose stimulated insulin secretion is mediated by glucose metabolism via oxidative phosphorylation generating ATP that triggers membrane depolarization and exocytosis of insulin. In stressed beta cells, remodeled, with enhanced glycolysis uncoupled from phosphorylation, resulting in the impaired glucose-mediated characteristic diabetes. Relative changes can be monitored living cells using 3-component fitting approach fluorescence lifetime imaging microscopy (FLIM). We engrafted pancreatic...