- Horticultural and Viticultural Research
- Genomics and Phylogenetic Studies
- Fermentation and Sensory Analysis
- Plant Virus Research Studies
- Yeasts and Rust Fungi Studies
- Plant Pathogens and Fungal Diseases
- Genetic Mapping and Diversity in Plants and Animals
- Plant and Fungal Interactions Research
- Plant-Microbe Interactions and Immunity
- Plant Disease Resistance and Genetics
- Chromosomal and Genetic Variations
- Analytical Methods in Pharmaceuticals
- Steroid Chemistry and Biochemistry
- Microbial infections and disease research
- RNA modifications and cancer
- Epigenetics and DNA Methylation
- Insect-Plant Interactions and Control
- Plant Pathogens and Resistance
- Plant biochemistry and biosynthesis
- Microbial metabolism and enzyme function
- Hemoglobin structure and function
- Plant Diversity and Evolution
- Plant and animal studies
- Diatoms and Algae Research
- Probiotics and Fermented Foods
Kurchatov Institute
2021-2025
Research Centre for Medical Genetics
2023-2024
Novosibirsk State University
2016-2018
Veterinary Research Institute
2006
The methylotrophic yeast Komagataella kurtzmanii belongs to the group of homothallic fungi that are able spontaneously change their mating type by inversion chromosomal DNA in MAT locus region. As a result, natural and genetically engineered cultures these yeasts typically contain mixture sexually dimorphic cells prone self-diploidisation spore formation accompanied genetic rearrangements. These characteristics pose significant challenge development stable producers for industrial use. In...
Phage Lydia, a newly isolated siphovirus infecting Pseudomonas aeruginosa, was characterized with respect to its basic kinetic properties and subjected comparative bioinformatic analysis related phages. The phage exhibited restricted host range, lytic activity observed against 7 of 30 tested isolates. genome Lydia consists 61,986 bp dsDNA molecule contains 89 predicted genes. Bioinformatic suggests the presence DNA modification system, but no apparent genes associated lysogeny or antibiotic...
B chromosomes (Bs) represent a variable addition to the main karyotype in some lineages of animals and plants. Bs accumulate through non-Mendelian inheritance become widespread populations. Despite presence multiple genes, most lack specific phenotypic effects, although their influence on host genome epigenetic status gene expression are recorded. Previously, using sequencing isolated ruminants rodents, we demonstrated that originate as segmental duplications genomic regions, subsequently...
In different countries, interest in the commercial cultivation of olive has recently greatly increased, which led to expansion its range. The Crimean Peninsula is northern limit common (Olea europaea L.) A unique collection olive's cultivars and hybrids been collected Nikitsky Botanical Gardens (NBG). aim this study was assess genetic diversity 151 samples (total several biological replicates 46 including 29 introduced 11 indigenous genotypes) using ddRAD sequencing method. Structural...
DNA sequence data from complete chloroplast genomes expand our understanding of plant diversity and contributes to evolutionary relationships among species. De novo assembly annotation are difficult due the high number repeats low in international databases. The development long-read sequencing platforms such as PacBio Oxford Nanopore, well specialized assemblers, is simplifying process assembling annotating large circular molecules. In this work, we report genome autochthonous Vitis...
9 Russian Vitis vinifera grape varieties and the European variety Muscat Hamburg were sequenced genotyped using 527 SNPs (single nucleotide polymorphisms) with high minor allele frequency for first time. The data coupled previously identified genotypes of 783 subjected to parentage population analysis. As a result, contrary historical ampelographic published in many sources from 1800 2012, only two nine (Pukhlyakovskiy Belyi Sibirkovyi) related foreign ones obviously imported Europe Empire....
About ten species of the genus Actinidia Lindl. are known as cultivated plants—alongside most A. chinensis var. deliciosa, there many others, including Far-Eastern cold-hardy kiwifruits such arguta, kolomikta, and polygama. Unlike plant species, in which chloroplast genome is maternally inherited, family Actinidiaceae possesses a complex system plastid inheritance with possible transmission through both maternal paternal lines. The main aim this work was assembly genomes three Actinidia,...
The autochthonous grape varieties of the Don Valley, situated in southern Russia, constitute a distinctive element regional cultural heritage. These have been adapted over centuries to region's specific local climatic and soil conditions. For most part, these are not imported from other countries. They closely related found Crimea North Caucasus. In this study, we obtained first complete, unfragmented sequences chloroplast genomes eight Valley one Crimea. We also performed comparative...
In this study, new classes of tandemly repeated DNA sequences in the centromeric regions three farm animal species are reported. Pericentromeric bovine chromosome X, porcine 14 and equine 1 were microdissected by laser amplified DOP-PCR. Painting probes showed strong hybridization signals on their corresponding centromeres. Specific fragments isolated cloning sequenced. For each species, high homology level was found among sequences. The presence specific repeats within sequence allows us to...
Prunus persica is one of the main stone fruit crops in Crimea and southern Russia. The P. genome has recently been sequenced annotated good quality. However, for a deeper assessment peach genome, it necessary to include research other cultivars that are collection Nikitsky Botanical Garden. Garden unique differ from Western European American ones, as they derived forms originating Central Asian, North Caucasian, Transcaucasian Eastern countries. In this paper, we present assembly P . cv....
We conducted a survey of the phytosanitary status Prunus germplasm collection in Nikita Botanical Gardens, Yalta, Russia. The virome plants displaying virus-like symptoms was studied using Illumina MiSeq high-throughput sequencing. Reads related to prunus necrotic ringspot virus (PNRSV), prune dwarf (PDV), and ourmia-like 1 (OuLV1) were generated number samples. Near complete genomes two divergent PNRSV isolates, PDV isolate, contig partly covered OuLV1 genome assembled de novo metaSPAdes...
Obesity is a complex, heterogeneous, actively progressive disease manifested by excessive formation of adipose tissue in the body and usually has high cardiometabolic risk specific complications. Currently, new data are emerging that explain pathogenesis obesity not only genetic variations imbalance between energy intake expenditure, but also influence epigenetic mechanisms, such as DNA methylation. methylation most studied modification, whose status cell can be altered various external...
More than ten species of the Actinidia Lindl. genus bear edible fruits rich in biologically active compounds, which are essential and beneficial for human health. The most popular cultivars today large-fruited species, A. deliciosa chinensis, commonly known as kiwi. However, small-fruited kiwi gaining prominence due to their high nutritional value, superior cold resistance, suitability temperate climates. In Russia, these represented by Far Eastern species: arguta, kolomikta, polygama....
Virus diseases affect the yield and fruit quality shorten productive life of stone fruits (Prunus spp. in family Rosaceae). Of over fifty known viruses infecting these crops, cherry virus A (CVA) is among most common, little 1 (LChV1) one economically important. Using high-throughput sequencing, full-length genomes CVA LChV1 isolates, found on interspecies hybrids Prunus collection Nikita Botanical Gardens, Russia, were sequenced, assembled, characterized. was P. cerasifera × armeniaca...
Probiotic supplements are currently widely used in cattle feeding practices. However, knowledge regarding the genomic landscape of probiotic microorganisms is relatively scarce and based on analogies with human probiotics. Here, we report draft genome sequences two Enterococcus lactis strains, VKPM B-4989 B-4992, which were isolated from rumen a healthy calf utilized as additive.
Acinetobacter pittii strain B-3780 is a prospective degrader of oil and methanol, isolated from industrial wastewater. Here, we present the draft genome sequence B-3780, obtained using Illumina sequencing fragment genomic library.
Bacillus velezensis Krd-20 strain with antifungal activity was isolated from the wheat rhizosphere. This is used to suppress fungi of Fusarium sp. when growing oyster mushroom (Pleurotus ostreatus). Genome assembling resulted in 44 contigs a total length 3939663 bp were obtained, GC content 46.4%.