A5088739814- T-cell and B-cell Immunology
- Immune Cell Function and Interaction
- CAR-T cell therapy research
- Immunotherapy and Immune Responses
- Protein Tyrosine Phosphatases
- Phagocytosis and Immune Regulation
- Cytomegalovirus and herpesvirus research
- Immune cells in cancer
- interferon and immune responses
- Neuroinflammation and Neurodegeneration Mechanisms
- Ubiquitin and proteasome pathways
- Galectins and Cancer Biology
- Mitochondrial Function and Pathology
- Chronic Lymphocytic Leukemia Research
Universidad Complutense de Madrid
2022-2025
Hospital Universitario de La Princesa
2022-2024
Universidad Autónoma de Madrid
2022-2024
Background Phosphatidylserine (PS) exposed on apoptotic cells promotes immune clearance of dead without inducing inflammation. Conversely, PS exposure live tumor an immunosuppressive microenvironment that hinders antitumor responses. After confirming elevated levels in various cell lines and cancer tissues, we aimed to investigate its potential as a target antigen for chimeric receptor T (CAR-T) therapy. Methods We used two different approaches PS. First, employed the adaptor proteins,...
Introduction CD8 + cytotoxic T lymphocytes (CTLs) are highly effective in defending against viral infections and tumours. They activated through the recognition of peptide–MHC-I complex by T-cell receptor (TCR) co-stimulation. This cognate interaction promotes organisation intimate cell–cell connections that involve cytoskeleton rearrangement to enable effector function clearance target cell. is key for asymmetric transport mobilisation lytic granules contact, promoting directed secretion...
Actin dynamics control early T-cell receptor (TCR) signalling during activation. However, the precise regulation of initial actin rearrangements is not completely understood. Here, we have investigated regulatory role phosphatase Slingshot-1 (SSH1) in this process. Our data show that SSH1 rapidly polarises to nascent cognate synaptic contacts and later relocalises peripheral F-actin networks organised at mature immunological synapse. Knockdown expression by CRISPR/Cas9-mediated genome...
The organization of the mitochondrial network is relevant for metabolic fate T cells and their ability to respond TCR stimulation. This arrangement depends on cytoskeleton dynamics in response CD28 activation, which allows polarization mitochondria through change shape, movement along microtubules towards immune synapse. work focus role End-binding protein 1 (EB1), a that regulates tubulin polymerization has been previously identified as regulator intracellular transport CD3-enriched...
<title>Abstract</title> Actin dynamics control early T cell receptor signalling during activation. However, the precise regulation of initial actin rearrangements is not completely understood. Here, we have investigated regulatory role phosphatase Slingshot-1 (SSH1) in this process. Our data show that SSH1 rapidly polarises to nascent cognate synaptic contacts and later relocalises peripheral F-actin networks organised at mature synapses. Knockdown expression by CRISPR/Cas9-mediated genome...
Abstract Phosphatases of regenerating liver (PRLs) have been proposed to regulate actin dynamics in lymphoid cells. However, the mechanism mediating this role remained unknown. Here we showed interaction PRLs with regulator WD repeat containing protein 1 (WDR1). The WDR1 was dependent on F-actin integrity and proper recruitment cell membranes through CAAX motif. Endogenous were distributed Immunological Synapse (IS) perturbed expression PRL-1 or PRL-2 by CRISPR/Cas9 mediated genome editing...