Mesenchymal stem cell (MSC) therapies for the treatment of diseases associated with inflammation and oxidative stress employ primarily bone marrow MSCs (BMMSCs) other MSC types such as from chorionic villi human term placentae (pMSCs). These are not derived microenvironments stress, unlike decidua basalis placenta (DBMSCs). DBMSCs were isolated then extensively characterized. Differentiation into three mesenchymal lineages (adipocytes, osteocytes, chondrocytes) was performed. Real‐time...

Coconut (Cocos nucifera L.), a member of the palm family (Arecaceae), is one most economically important crops in tropics, serving as an source food, drink, fuel, medicine, and construction material. Here we report assembly coconut (C. nucifera, Oman local Tall cultivar) mitochondrial (mt) genome based on next-generation sequencing data. This genome, 678,653bp length 45.5% GC content, encodes 72 proteins, 9 pseudogenes, 23 tRNAs, 3 ribosomal RNAs. Within assembly, find that chloroplast (cp)...

Abstract Background Lung transplantation is a life-saving surgical replacement of diseased lungs in patients with end-stage respiratory malfunctions. Despite remarkable short-term recovery, long-term lung survival continues to face several major challenges, including chronic rejection and severe toxic side effects due global immunosuppression. Stem cell-based immunotherapy has been recognized as crucial immunoregulatory regimen various preclinical clinical studies. initial therapeutic...

Human decidua basalis mesenchymal stem/multipotent stromal cells (DBMSCs) inhibit endothelial cell activation by inflammation induced monocytes. This property makes them a promising candidate for cell-based therapy to treat inflammatory diseases, such as atherosclerosis. study was performed examine the ability of DBMSCs protect functions from damaging effects resulting exposure oxidatively stress environment H2O2 and were co-cultured with isolated human umbilical cord veins in presence...

Placental mesenchymal stem cells from maternal decidua basalis tissue (DBMSCs) are promising for repair because of their multilineage differentiation and ability to protect endothelial injury. Here, we examined DBMSC interaction with macrophages whether this could modulate the characteristics functions these macrophages. We induced monocytes differentiate into M1-like in presence DBMSCs. effects on were evaluated using microscopy, flow cytometry, ELISA. macrophage induction T cell function...

Rhazya stricta Decne. is a medicinal plant that widespread in Saudi Arabia and desert areas of the Arabian Peninsula. Its extract contains alkaloids, tannins, flavonoids are involved different biological activities. The study aim was to evaluate effects extracts on proliferation differentiation NTERA-2 (NT2) pluripotent embryonal carcinoma cells.Soxhlet extraction carried out using solvents stems, leaves fruit parts this plant. Cytotoxicity evaluated by an MTS cell viability assay. ability...

Recently, we reported the isolation and characterization of mesenchymal stem cells from decidua basalis human placenta (DBMSCs). These express a unique combination molecules involved in many important cellular functions, which make them good candidates for cell-based therapies. The endothelium is highly specialized, metabolically active interface between blood underlying tissues. Inflammatory factors stimulate to undergo change proinflammatory procoagulant state (ie, endothelial cell...

Human decidua parietalis mesenchymal stem/multipotent stromal cells (DPMSCs) have unique phenotypic and functional properties that make them promising candidates for cell-based therapy. Here, we investigated DPMSC interaction with natural killer (NK) cells, the effects of this on NK cell characteristics activities. DPMSCs isolated from human fetal membranes were cultured interleukin (IL)-2-activated IL-2-unactivated healthy peripheral blood. proliferation cytolytic activities then examined...

Gold(I) complexes of 2-(diphenylphosphanyl)ethylamine or (2-aminoethyl)diphenylphosphine (AEP), and dithiocaarbamates (R2NCS2) were prepared by the reaction these ligands with (CH3)2S-AuCl in dichloromethane. The synthesized [Au(AEP)Cl] (1), [Au(AEP)2]Cl (2), [Au2(R2NCS2)2]n (R2 = dimethyl (3), diethyl (4), dibenzyl (5)) characterized elemental analysis, IR, 1H, 13C 31P NMR spectroscopy. evaluated for anticancer activity against three cancer cells, A549 (human lung carcinoma), HCT15 colon...

We investigated whether polymorphism rs7555523 (A > C) in human transmembrane and coiled-coil domain 1 (TMCO1) gene is a risk factor for primary open angle glaucoma (POAG) Saudi cohort. A cohort of 87 unrelated POAG cases 94 control subjects from Arabia were genotyped using Taq-Man® assay. The association genotypes with other specific clinical indices was investigated. genotype allele frequency at TMCO1 did not show any statistically significant as compared to controls. minor 0.103 0.085...

To investigate whether the polymorphism rs1063192 (A>G) in cyclin-dependent kinase Inhibitor-2B (CDKN2B) gene is a risk factor for primary open-angle glaucoma (POAG).A case-control study was conducted wherein we genotyped 87 unrelated POAG cases and 94 control subjects from Saudi Arabia using Taq-Man® assay.The minor allele frequency 0.20 0.21 controls. Both genotype frequencies were not significantly different between No significant association found genotypes clinical indices, except that...

The SKiPSc1 induced pluripotent stem (iPS) cell line was generated from Human Neonatal Foreskin Fibroblasts (HNFFs) obtained a healthy donor infant that were reprogrammed using non-integrating Sendai viral vectors expressing Oct3/4, Sox2, c-Myc, and Klf4.

Gold(<sc>iii</sc>) complexes were tested for <italic>in vitro</italic> antiproliferative activity against three human tumor cell lines. All exhibited remarkable cytotoxicity.

Many studies have been done and many results established for studying cancers in human the ways of treating it. However, one thing that remains relevant is study model used to diagnose, cure or conclude treatment methods cancers. The scientists tried some link data analyze malicious disease various animal models order solve problem humans. Out all models, preferred dogs as most suitable conducted on them. Our article will review reason preferences given dog a what previous by considering...

ABSTRACT In this paper we introduce a decision support system framework termed the Integrated Energy Decision Support System (IEDSS). IEDSS was developed for energy planning at national and regional levels to inform planners multiple of government. employs dynamics modeling enable rapid evaluation outcomes different supply demand policies level. Agent‐Based models are used mimic interactions between entities when applying level Within policy makers specify set decisions choose from uncertain...

To investigate the association between rs1900004 polymorphism in atonal bHLH transcription factor 7 (ATOH7) gene and primary open angle glaucoma (POAG) Saudi patients.Eighty-seven unrelated POAG cases 94 control subjects of origin were genotyped utilizing a TaqMan® assay. The mutant genotypes its related clinical indices was investigated.The genotype allele frequencies ATOH7 did not show any statistically significant with compared to controls. minor frequency 0.32 both None demographic,...

Although the sequencing information of Sox2 cDNA for many mammalian is available, Camelus dromedaries has not yet been characterized. The objective this study was to sequence and characterize from brain C. dromedarius (also known as Arabian camel). A full coding gene amplified by reverse transcription PCRjmc then sequenced using 3730XL series platform Sequencer (Applied Biosystem) first time. displayed an open reading frame 822 nucleotides, encoding a protein 273 amino acids. molecular...

DPPA4 is essential for the pluripotent stem cell state, yet its function poorly understood. localized in nucleus, where it associated with active chromatin. We now report that also present cytosol, appears as diffused clouds, distinct foci and sometimes spaghetti-like structures. This cytosolic localization dynamic shuttles between cytosol nucleus. Its presence almost abolished from nucleus upon differentiation. Co-immunoprecipitation studies highlighted novel protein interactors, many of...

Camelids skin is claimed to respond differently under different physiological and pathological condition, however, there no in vitro model with a reporter investigate this claim. So generating camel fibroblast cell lines that expresses green fluorescent protein (GFP) would be important as tool monitor growth, migration other processes. Hence, investigation, we created GFP expressing derived from primary lung of the Arabian stable expression which was transfected using pCAG-EGFP plasmid CMV...

MSCs produce CD4(+)CD25(+)FOX3(+) regulatory T (Treg) cells from activated peripheral blood mononuclear (PBMC), T-CD4+ and T-CD8+ in vitro vivo. Here we investigated whether the deficiency of androgen/AR influence Treg induction total PBMC, splenocytes, CD4+CD25-through AR/TGF-β interaction. Eight to 12-week-old wild type general androgen receptor knockout (ARKO) mice were used. collected, characterized function was studied. Our result showed that depletion AR suppressed immunosuppressive...