A5016815495- Ultrasound and Hyperthermia Applications
- Photoacoustic and Ultrasonic Imaging
- Dementia and Cognitive Impairment Research
- T-cell and B-cell Immunology
- Immunotherapy and Immune Responses
- Functional Brain Connectivity Studies
- Reproductive System and Pregnancy
- Cardiovascular Disease and Adiposity
- interferon and immune responses
- Immune Response and Inflammation
- Neuroinflammation and Neurodegeneration Mechanisms
- Ultrasound and Cavitation Phenomena
- Adipokines, Inflammation, and Metabolic Diseases
- Cardiovascular and Diving-Related Complications
- Advanced Neuroimaging Techniques and Applications
- Thermoregulation and physiological responses
- Health, Environment, Cognitive Aging
- Salivary Gland Tumors Diagnosis and Treatment
- Reproductive Physiology in Livestock
- Anesthesia and Neurotoxicity Research
- Diet and metabolism studies
- Viral Infections and Vectors
- Immune Cell Function and Interaction
- Pregnancy and preeclampsia studies
- Xenotransplantation and immune response
Mallinckrodt (United States)
2023-2025
Washington University in St. Louis
2023-2024
Allegheny General Hospital
2024
Novartis (United States)
2023
University of Pittsburgh
2017-2022
University of Pittsburgh Medical Center
2021
McGowan Institute for Regenerative Medicine
2017
University Health Network
2009
University of Toronto
2000-2008
Toronto General Hospital
1999-2008
The cloning, expression, and function of the murine (m) homologue human (h) monocyte-derived chemokine (MDC) is reported here. Like hMDC, mMDC able to elicit chemotactic migration in vitro activated lymphocytes monocytes. Among lymphocytes, Th2 cells were induced migrate most efficiently. mRNA protein expression modulated during course an allergic reaction lung. Neutralization with specific Abs a model lung inflammation resulted prevention airway hyperreactivity significant reduction...
Abstract Stimulator of interferon genes (STING) is a dimeric transmembrane adapter protein that plays key role in the human innate immune response to infection and has been therapeutically exploited for its antitumor activity. The activation STING requires high-order oligomerization, which could be induced by binding endogenous ligand, cGAMP, cytosolic ligand-binding domain. Here we report discovery through functional screens class compounds, named NVS-STGs, activate STING. Our cryo-EM...
We have established that, in mice receiving donor-specific immunization by the portal vein, increased graft survival seen is associated with expression of a molecule (OX-2) on subpopulation dendritic cells (DC), and polarization cytokine production to type 2 cytokines Ag-specific restimulation from these mice. Furthermore, infusion mAb OX-2 blocks both altered seen. constructed an immunoadhesin which extracellular domain linked murine IgG2a Fc region, we expressed this (OX-2:Fc) eukaryotic...
Cell-loaded hydrogels are frequently applied in cartilage tissue engineering for their biocompatibility, ease of application, and ability to conform various defect sites. As a bioactive adjunct the biomaterial, transforming growth factor beta (TGF-β) has been shown be essential cell differentiation into chondrocyte phenotype maintenance thereof, but low amounts endogenous TGF-β vivo joint microenvironment necessitate mechanism controlled delivery release this factor. In study, TGF-β3 was...
PROBLEM: At least two dendritic cell‐associated molecules have been shown to contribute the successful outcome of organ and tissue allografts in mice, namely CD200 MD‐1. is up‐regulated rodent transplantation models where inhibition rejection accomplished, believed signal immunosuppression following engagement a receptor, CD200R, on macrophages and/or γδ T‐cell receptor (γδ TCR + cells MD‐1 implicated controlling expression costimulatory including CD80/CD86 which induce an immunorejection...
Obesity is related to quantitative neuroimaging abnormalities including reduced gray matter volumes and impaired white microstructural integrity, although the underlying mechanisms are not well understood. We assessed influence of obesity on neuroinflammation imaging that may mediate brain morphometric changes. Establishing role in will enhance understanding this modifiable disorder as a risk factor for Alzheimer's disease (AD) dementia. analyzed MRIs from 104 cognitively normal participants...
Obesity and excess adiposity at midlife are risk factors for Alzheimer disease (AD). Visceral fat is known to be associated with insulin resistance a pro-inflammatory state, the two mechanisms involved in AD pathology. We assessed association of obesity, MRI-determined abdominal adipose tissue volumes, PET-determined amyloid tau uptake default mode network areas, brain volume cortical thickness signature cognitively normal population. Thirty-two middle-aged (age: 51.27±6.12 years, 15 males,...
Problem Lipopolysaccharide (LPS) acts via tlr4 to promote Th1 cytokine secretion and abortions. LPS is an essential co‐factor in spontaneous abortion the CBA × DBA/2 model stress‐triggered In model, C3a, C5a, fgl2 prothrombinase participate triggering inflammation that terminates embryo viability. As (via thrombin) can generate it was predicted LPS‐driven abortions (which require fgl2) would be independent of C3. CD200Fc prevent but action through Fc could not excluded. Method study C3 −/−...
Fetal loss syndrome (abortion/resorption) occurring on or after gestation day (gd) 9.5 in CBA/JxDBA/2 matings is dependent upon presence of TNF-alpha + IFN-gamma, which act by increasing expression fg12 prothrombinase at the feto-maternal interface. The magnitude abortion rate can be boosted an injection these cytokines gd 7.5 depends endogenous loss, and appears to depend microbial flora. Is cytokine-triggered a third signaling pathway that senses 'danger'?Female CBA/J were mated DBA/2...
We screened a library of bioactive small molecules for activators and inhibitors innate immune signaling through IRF3 NFkB pathways with the goals advancing pathway understanding discovering probes immunology research. used high content screening to measure translocation from cytoplasm nucleus in primary human macrophages; these transcription factors play critical role activation STING other pro-inflammatory pathways. Our activator screen yielded diverse set hits that promoted nuclear and/or...
Additive manufacturing presents opportunities to treat bone defects using biomimetic tissue scaffolds. Past investigations have explored modulating scaffold mechanical properties through varying materials and geometric motifs. Herein, we applied the rotated plywood structure of a 3D printed with goal improving performance compared an orthogonal mesh design commonly used in applications. The scaffolds were subjected uniaxial compression followed by scanning electron microscopy microcomputer...
Rationale:The treatment of microvascular obstruction (MVO) using ultrasound-targeted LNP cavitation (UTC) therapy mechanically relieves the physical in microcirculation but does not specifically target associated inflammatory milieu.Electrophilic fatty acid nitroalkene derivatives (nitro-fatty acids), that display pleiotropic anti-inflammatory signaling and transcriptional regulatory actions, offer strong therapeutic potential lack a means rapid targeted delivery.The objective this study was...
This study investigated how obesity, BMI ≥ 30 kg/m
Abstract The immunoadhesin (OX2:Fc) comprising the extracellular domain of murine OX2 linked to IgG2aFc, inhibits production IL-2 and IFN-γ by activated T cells increases allograft xenograft survival in vivo. Increased expression on dendritic (DC) vivo following preimmunization via portal vein is also associated with elevated MD-1. We have used antisense oligodeoxynucleotides (ODNs) MD-1 investigate effect inhibition DC their function as allostimulatory cells. investigated FACS analysis cell...