A5052792869- Neuropeptides and Animal Physiology
- Gastrointestinal motility and disorders
- Nitric Oxide and Endothelin Effects
- Receptor Mechanisms and Signaling
- Adenosine and Purinergic Signaling
- Ion channel regulation and function
- Muscle Physiology and Disorders
- Neuroscience of respiration and sleep
- Diet and metabolism studies
- Pain Mechanisms and Treatments
- Ion Channels and Receptors
- Neuroscience and Neuropharmacology Research
- Neuroendocrine regulation and behavior
- Hormonal Regulation and Hypertension
- Gastroesophageal reflux and treatments
- Pharmacological Effects and Assays
- Renin-Angiotensin System Studies
- Cannabis and Cannabinoid Research
- Biochemical Analysis and Sensing Techniques
- Pharmacological Receptor Mechanisms and Effects
- Adipose Tissue and Metabolism
- Diabetes Treatment and Management
- Infant Health and Development
- Helicobacter pylori-related gastroenterology studies
- Inflammatory mediators and NSAID effects
University of Palermo
2015-2024
University of Genoa
2023
Institute of Cell Biology and Neurobiology
1985-2010
University of Bari Aldo Moro
2000
McMaster University
1988-1991
Istituto di Fisiologia Clinica
1982-1990
McMaster University Medical Centre
1988
Health Sciences Centre
1988
Merck & Co., Inc., Rahway, NJ, USA (United States)
1984
United States Military Academy
1984
Background Glucagon-like peptide-1 (GLP-1) is a proglucagon-derived peptide expressed in the enteroendocrine-L cells of small and large intestine released response to meal ingestion. exerts inhibitory effects on gastrointestinal motility through vagal afferents central nervous mechanisms; however, no data available about direct influence wall. Our aim was investigate GLP-1 spontaneous evoked mechanical activity mouse duodenum colon identify presence distribution receptors (GLP-1R) muscle...
Plant-derived nanovesicles (PDNVs) have recently emerged as natural delivery systems of biofunctional compounds toward mammalian cells. Considering their already described composition, anti-inflammatory properties, stability, and low toxicity, PDNVs offer a promising path for developing new preventive strategies several inflammatory diseases, among which the bowel disease (IBD). In this study, we explore protective effects industrially produced lemon vesicles (iLNVs) in rat model IBD....
Glucagon-like peptide-2 (GLP-2) influences different aspects of the gastrointestinal function, including epithelial growth, digestion, absorption, motility, and blood flow. Intraluminal pressure from isolated mouse stomach was recorded to investigate whether GLP-2 affects gastric tone analyze its mechanism action. Regional differences between diverse parts were also examined using circular muscular strips fundus antrum. In whole stomach, (0.3-100 nM) produced concentration-dependent...
Duchenne muscular dystrophy (DMD), which results from deficiency in dystrophin, a sarcolemma protein of skeletal, cardiac and smooth muscle, is characterized by progressive striated muscle degeneration, but various gastrointestinal clinical manifestations have been observed. The aim was to evaluate the possible impact dystrophin loss on propulsion mdx mice (animal model for DMD). gastric emptying carboxymethyl cellulose/phenol red dye non-nutrient meal not significantly different at 20 min...
Glucagon-like peptide 1 (GLP1) is a naturally occurring secreted by intestinal L-cells. Though its primary function to serve as an incretin, GLP1 reduces gastrointestinal motility. However, only handful of animal studies have specifically evaluated the influence on colonic Consequently, aims this study were investigate effects induced exogenous GLP1, analyze mechanism action, and verify presence receptors (GLP1Rs) in human colon circular muscular strips. Organ bath technique, RT-PCR, western...
The neurotransmitters involved in NANC relaxation and their possible interactions were investigated mouse isolated stomach, recording the motor responses as changes of endoluminal pressure from whole organ. Field stimulation produced tetrodotoxin‐sensitive, frequency‐dependent, biphasic responses: rapid transient followed by a delayed inhibitory component. inhibitor synthesis nitric oxide (NO), L ‐NAME, abolished significantly reduced slow relaxation. Apamin, blocker Ca 2+ ‐dependent K +...
The present study compares the electrophysiological properties of two preparations dissected from canine colon circular muscle layer: first, containing submucosal network interstitial cells Cajal (ICC) with to four associated smooth cell layers, and second, a preparation devoid ICC network. In ICC-rich preparations, consistent slow-wave activity was observed prolonged plateau potentials approximately 10-s duration. were sensitive D 600. 45% (confirmed using electron microscopy) slow waves,...
We investigated the potential anorectic action of peripherally administered glucagon-like peptide 2 (GLP2) in lean and diet-induced obese (DIO) mice. Mice, fasted for 16 h, were injected i.p. with native GLP2 or [Gly ]GLP2, stable analog GLP2, before after (3–33), a receptor (GLP2R) antagonist, exendin (9–39), GLP1R antagonist. Food intake was measured at intervals 1, 2, 4, 8, 24 h postinjection. In addition, we tested mice influence ]GLP2 on gastric emptying effects GLP1 alone combination...
This study analysed the contribution of purinergic system to enteric neurotransmission in longitudinal muscle mouse distal colon.Motor responses exogenous ATP and nerve stimulation vitro were assessed as changes isometric tension.ATP induced a concentration-dependent contraction, reduced by 4-[[4-formyl-5-hydroxy-6-methyl-3-[(phosphonooxy)methyl]-2-pyridinyl]azo]-1,3-benzene disulphonic acid (PPADS), suramin, P2Y purinoreceptor desensitisation with adenosine 5'-O-2-thiodiphosphate...
We investigated, using an organ bath technique, the effects of a hydrophilic extract from Opuntia ficus indica fruit pulp (cactus extract, CFE) on motility mouse ileum, and researched component(s) responsible for observed responses. CFE (10−320 mg fresh equivalents/mL bath) reduced dose-dependently spontaneous contractions. This effect was unaffected by tetrodotoxin, neuronal blocker, Nω-nitro-l-arginine methyl ester, nitric oxide synthase tetraethylammonium, potassium channel or atropine,...
The aim of this study was to compare the motor pattern (recorded as changes in intraluminal pressure) isolated duodenum and proximal colon between dystrophic mdx normal mice. When duodenal recordings from control preparations were compared with mice there no significant difference spontaneous pattern, responses electrical nerve stimulation or sensitivity pharmacological agents. Colonic segments showed a more complex consisting contractions amplitude frequency similar those controls by...
The objective of this study is to determine which inflammatory mediators had direct effects on canine trachealis muscle neuromuscular control identify candidate the hyperresponsiveness observed in vitro after O3 exposure. Studies were carried out sucrose gap at 29 degrees C and bath 37 C. Leukotriene (LT) B4, LTD4, prostaglandin (PG) D2 neither nor significant excitatory junction potentials (EJP's), secondary membrane potential oscillations, or associated contractions that followed field...