A5006477780- HIV Research and Treatment
- Immune Cell Function and Interaction
- T-cell and B-cell Immunology
- Cytomegalovirus and herpesvirus research
- Viral Infections and Vectors
- Vector-borne infectious diseases
- Asthma and respiratory diseases
- Invertebrate Immune Response Mechanisms
- Infant Nutrition and Health
- Animal Virus Infections Studies
- Virology and Viral Diseases
- Advanced Biosensing Techniques and Applications
- Respiratory Support and Mechanisms
- Immune Response and Inflammation
- HIV-related health complications and treatments
- Hepatitis C virus research
- Monoclonal and Polyclonal Antibodies Research
- HIV/AIDS drug development and treatment
- Inhalation and Respiratory Drug Delivery
- HER2/EGFR in Cancer Research
University of Colorado Denver
2017-2023
Ventana Research Corporation (United States)
2022
George Mason University
2008-2013
University of Utah
2003-2008
Almost all viral pathogens utilize a cytoskeleton for their entry and intracellular transport. In HIV-1 infection, binding of the virus to blood resting CD4 T cells initiates temporal course cortical actin polymerization depolymerization, process mimicking chemotactic response initiated from chemokine receptors. The depolymerization has been suggested promote migration through cofilin-mediated treadmilling. However, role virus-mediated in HIV infection is unknown, signaling molecules...
HIV fusion and entry into CD4 T cells are mediated by two receptors, CXCR4. This receptor requirement can be abrogated pseudotyping the virion with vesicular stomatitis virus glycoprotein (VSV-G) that mediates viral through endocytosis. The VSV-G-pseudotyped is highly infectious for transformed cells, although circumvents receptors actin cortex. In infection, gp120 binding to also transduces signals. Recently, we demonstrated a unique CXCR4 signaling in latent infection of blood resting...
Cofilin is an actin-depolymerizing factor that regulates actin dynamics critical for T cell migration and activation. In unstimulated resting CD4 cells, cofilin exists largely as a phosphorylated inactive form. Previously, we demonstrated during HIV-1 infection of the viral envelope-CXCR4 signaling activates to overcome static cortical restriction. this pilot study, have extended in vitro observation examined phosphorylation cells purified from peripheral blood HIV-1-infected patients. Here,...
Previous studies have observed fluorescently labeled HIV particles tracking along microtubule networks for nuclear localization. To provide direct evidence the involvement of microtubules in early steps infection human CD4 T cells, we used multiple modulators such as paclitaxel (originally called taxol; 1 μM), vinblastine (1 and 10 colchicine (10 100 nocodazole μM) to disturb transformed resting cells. Although these drugs disrupted integrity, almost no inhibition HIV-1 was observed. Our...
Global transcriptome studies can help pinpoint key cellular pathways exploited by viruses to replicate and cause pathogenesis. Previous data showed that laboratory-adapted HIV-1 triggers significant gene expression changes in CD4+ T cell lines mitogen-activated cells from peripheral blood. However, primarily targets mucosal compartments during acute infection vivo. Moreover, early causes extensive depletion of the gastrointestinal tract herald persistent inflammation due translocation...
C57BL/6 mice deficient in TLR2 develop more severe arthritis following infection with Borrelia burgdorferi than do wild-type mice, and this increase is suppressed by the simultaneous presence of scid mutation. This suggested a requirement for lymphocytes development subacute Lyme TLR2−/− feature not commonly associated arthritis. The increased pathology B. burgdorferi-infected was also accompanied an mononuclear cell infiltration. In study, T cells were found to be responsible infected C3H...
Abstract Background Binding of HIV to the chemokine coreceptor CXCR4 mediates viral fusion and signal transduction that promotes actin dynamics critical for infection blood resting CD4 T cells. It has been suggested this gp120-mediated activity resembles chemotactic mediated by chemokines such as SDF-1. To determine whether inhibiting SDF-1-mediated can also inhibit infection, we screened several inhibitors known reduce chemotaxis Results We found a tyrosine kinase inhibitor, genistein,...
ABSTRACT Toll-like receptor 2 (TLR2) is a transmembrane signal transducer for tripalmitoyl- S -glyceryl-cysteine (Pam 3 Cys)-modified lipoproteins, including OspA from the Lyme disease spirochete Borrelia burgdorferi . The Pam Cys modification provides adjuvant activity inducing humoral responses, suggesting that TLR2 could function as vaccine. importance of in response to was confirmed, because overall levels immunoglobulin G (IgG) were reduced TLR2-deficient mice, when compared with those...
Background: Identification of HER2 protein overexpression and/or amplification the gene are required to qualify breast cancer patients for targeted therapies. In situ hybridization (ISH) assays that identify function as a stand-alone test determination status and rely on manual quantification number genes copies chromosome 17 determine amplification. Methods: To assist pathologists, we have developed uPath Dual ISH Image Analysis Breast (uPath DISH IA) algorithm, an adjunctive aid in...
We have previously demonstrated that binding of HIV-1 to the chemokine coreceptor, CXCR4, on resting CD4 T cells activates an actin-depolymerizing factor cofilin promote cortical actin dynamic critical for HIV latent infection [1]. The LIM domain kinase 1 (LIMK1) directly phosphorylates and regulates cytoskeleton. Here, we investigated role LIMK1 in found triggered a rapid, transient activation LIMK1. also used siRNA knockdown inhibit activity caused decrease F-actin cell chemotaxis. had...