A5078880154- Peroxisome Proliferator-Activated Receptors
- Immune cells in cancer
- Immune Cell Function and Interaction
- Histone Deacetylase Inhibitors Research
- Cancer Cells and Metastasis
- Cancer Research and Treatments
- Cancer-related gene regulation
- Angiogenesis and VEGF in Cancer
- Metabolism, Diabetes, and Cancer
- MicroRNA in disease regulation
- Cell Adhesion Molecules Research
- TGF-β signaling in diseases
- Cytokine Signaling Pathways and Interactions
- Cancer, Lipids, and Metabolism
- Bioinformatics and Genomic Networks
- Immune Response and Inflammation
- Inflammatory mediators and NSAID effects
- Estrogen and related hormone effects
- Cancer, Hypoxia, and Metabolism
- Extracellular vesicles in disease
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Cholangiocarcinoma and Gallbladder Cancer Studies
- Lung Cancer Research Studies
- Pancreatic and Hepatic Oncology Research
- Cancer Genomics and Diagnostics
University of Michigan
2015-2025
Ann Arbor VA Medical Center
2025
Pulmonary and Critical Care Associates
2004-2023
Michigan Medicine
2009-2021
Michigan United
2018
Northeast Ohio Medical University
2014
Ann Arbor Center for Independent Living
2012
UCLouvain
2010
de Duve Institute
2010
Michigan Cancer Research Consortium
2009
Transforming growth factor-β (TGF-β) induces epithelial-mesenchymal transition (EMT) of epithelial cells in both normal embryonic development and certain pathological contexts. Here, we show that TGF-β induced-EMT human lung cancer (A549; adenocarcinoma cells) mediates tumor cell migration invasion phenotypes. To gain insights into molecular events during EMT, employed a global stable isotope labeled profiling strategy using iTRAQ reagents, followed by 2DLC−MS/MS, which identified total 51...
Transforming growth factor-β1 (TGF-β1) is a multifunctional cytokine involved in differentiation, growth, and survival of mesenchymal cells while inhibiting growth/survival most other cell types. The mechanism(s) pro-survival signaling by TGF-β1 unclear. In this report, we demonstrate that protects against serum deprivation-induced apoptosis isolated from patients with acute lung injury normal human fetal fibroblasts (IMR-90). TGF-β receptor(s)-activated these involves rapid activation the...
Pulmonary fibrosis is characterized by alterations in fibroblast phenotypes resulting excessive extracellular matrix accumulation and anatomic remodeling. Current therapies for this condition are largely ineffective. Peroxisome proliferator-activated receptor-gamma (PPAR-gamma) a member of the nuclear hormone receptor superfamily, activation which produces number biological effects, including metabolic inflammatory responses. The role PPAR-gamma as potential therapeutic target fibrotic lung...
Abstract Motivation: The elucidation of biological concepts enriched with differentially expressed genes has become an integral part the analysis and interpretation genomic data. Of additional importance is ability to explore networks relationships among previously defined from diverse information sources, results visually multiple perspectives. Accomplishing these tasks requires a unified framework for agglomeration data various resources, novel visualizations, user functionality. Results:...
Myofibroblasts are crucial to the pathogenesis of tissue fibrosis. Their formation stress fibers results in release myocardin-related transcription factor (MRTF), a transcriptional coactivator serum response (SRF). MRTF-A (<i>Mkl1</i>)-deficient mice protected from lung We hypothesized that SRF/MRTF pathway inhibitor CCG-203971 would modulate myofibroblast function <i>in vitro</i> and limit fibrosis vivo</i>. Normal idiopathic pulmonary fibroblasts were treated with/without...
Abstract Epithelial-mesenchymal transition (EMT) was shown to confer tumor cells with abilities essential for metastasis, including migratory phenotype, invasiveness, resistance apoptosis, evading immune surveillance, and stem cell traits. Therefore, inhibition of EMT can be an important therapeutic strategy inhibit metastasis. Here, we show that activation peroxisome proliferator-activated receptor γ (PPAR-γ) inhibits transforming growth factor β (TGF-β)-induced in lung cancer prevents...
During epithelial-mesenchymal transition (EMT) epithelial cancer cells transdifferentiate into highly motile, invasive, mesenchymal-like cells, giving rise to disseminating tumor cells. Few of these disseminated successfully metastasize. Immune and inflammation in the microenvironment were shown drive EMT, but few studies investigated consequences EMT for immunosurveillance. In addition initiating metastasis, we demonstrate that confers increased susceptibility natural killer (NK)...
As the recognition between natural killer (NK) cells and cancer does not require antigen presentation, NK are being actively studied for use in adoptive cell therapies rapidly evolving armamentarium of immunotherapy. In addition to utilizing cells, recent studies have shown that exosomes derived from also exhibit antitumor properties. Furthermore, these cell-derived higher stability, greater modification potentials less immunogenicity compared cells. Therefore, technologies allow highly...
Activation of mitogen-activated protein kinase (Erk/MAPK) is a critical signal transduction event for estrogen (E<sub>2</sub>)-mediated cell proliferation. Recent studies from our group and others have shown that persistent activation Erk plays major role in migration tumor progression. The signaling mechanism(s) responsible are not fully characterized, however. In this study, we E<sub>2</sub> induces slow but MCF-7 breast carcinoma cells. E<sub>2</sub>-induced dependent on new synthesis,...
Activation of peroxisome proliferator-activated receptor-γ (PPAR-γ) results in inhibition tumor growth various types cancers, but the mechanism(s) by which PPAR-γ induces arrest has not been completely defined. In a recent study, we demonstrated that treatment A549 (human non small cell lung cancer line) tumor-bearing SCID mice with ligands troglitazone (Tro) and pioglitazone significantly inhibits primary growth. this immunohistochemical analysis Tro-treated Pio-treated tumors factor VIII...
Background The ability to selectively detect and target cancer cells that have undergone an epithelial-mesenchymal transition (EMT) may lead improved methods treat cancers such as pancreatic cancer. remodeling of cellular glycosylation previously has been associated with cell differentiation represent a valuable class molecular targets for EMT. Methodology/Principal Findings As first step toward investigating the nature alterations in EMT, we characterized expression glycan-related genes...
To gain insights into how TGF-beta regulates epithelial-mesenchymal transition (EMT), we assessed the time course of proteins and mRNAs during EMT by multiplex iTRAQ labeling 2D-LC-MS/MS, hybridization, respectively. Temporal analysis identified 66 as differentially expressed EMT, including newly associated calpain, fascin macrophage-migration inhibitory factor (MIF). Comparing protein mRNA expression overtime showed that all 14 up-regulated involved in actin-cytoskeleton remodeling were...
Significance Cysteinyl leukotrienes (cys-LTs) are proinflammatory mediators and regulate endothelial cell contraction, permeability, angiogenesis via a cys-LT receptor, CysLT 2 R. In response to the challenges of finding therapies that can combat tumor metastases, we explored advantage targeting this receptor. We show R mediates contributes metastasis in vivo independent VEGF by enhancing leakiness permeability blood vessels. propose antagonist, BayCysLT , normalize vessels, reducing growth...