A5073269014- Extracellular vesicles in disease
- MicroRNA in disease regulation
- Nanopore and Nanochannel Transport Studies
- Microfluidic and Bio-sensing Technologies
- 3D Printing in Biomedical Research
- Cancer Cells and Metastasis
- Radiomics and Machine Learning in Medical Imaging
- Gastrointestinal Bleeding Diagnosis and Treatment
- Lung Cancer Diagnosis and Treatment
- Lung Cancer Treatments and Mutations
- Micro and Nano Robotics
- Single-cell and spatial transcriptomics
- Nanoplatforms for cancer theranostics
- Innovative Microfluidic and Catalytic Techniques Innovation
- Immune cells in cancer
- Gold and Silver Nanoparticles Synthesis and Applications
- Cell Adhesion Molecules Research
- Lung Cancer Research Studies
- Cancer Immunotherapy and Biomarkers
- Additive Manufacturing and 3D Printing Technologies
- Circular RNAs in diseases
- Cancer Genomics and Diagnostics
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Biosensors and Analytical Detection
- Genetic and Kidney Cyst Diseases
University of Michigan
2019-2024
BioSurfaces (United States)
2019-2024
Stanford University
2022-2024
Ann Arbor Center for Independent Living
2019-2020
Duke University
2020
Vanderbilt University
2020
Abstract Extracellular vesicles (EVs) are emerging as a potential diagnostic test for cancer. Owing to the recent advances in microfluidics, on‐chip EV isolation is showing promise with respect improved recovery rates, smaller necessary sample volumes, and shorter processing times than ultracentrifugation. Immunoaffinity‐based microfluidic using anti‐CD63 widely used; however, not specific cancer‐EVs, some cancers secrete EVs low expression of CD63. Alternatively, phosphatidylserine (PS),...
As the recognition between natural killer (NK) cells and cancer does not require antigen presentation, NK are being actively studied for use in adoptive cell therapies rapidly evolving armamentarium of immunotherapy. In addition to utilizing cells, recent studies have shown that exosomes derived from also exhibit antitumor properties. Furthermore, these cell-derived higher stability, greater modification potentials less immunogenicity compared cells. Therefore, technologies allow highly...
Immunoaffinity based EV isolation technologies use antibodies targeting surface markers on EVs to provide higher specificity and purity compared existing approaches.
Melanoma is among the most aggressive cancers, and its rate of incidence continues to grow. Early detection melanoma has been hampered due lack promising markers for testing. Recent advances in liquid biopsy have proposed noninvasive alternatives cancer diagnosis monitoring. Circulating tumor cells (CTCs) cancer-exosomes are gaining influence as biomarkers because their cancer-associated molecular signatures. However, technologies that offer dual-isolation CTCs exosomes using a single sample...
Abstract Biohybrid microrobotics are exploited as a delivery approach to actively transport therapeutic payload tumors. However, the utility of this can be diminished by tumor hypoxia. Moreover, current biohybrid design is mainly focused on living organisms’ motility and biocompatibility, yet unique biological function natural organism often overlooked. Here, an all‐in‐one self‐propelled volvox‐based multifunctional robot, that is, Volbot, with built‐in capabilities fluid mixing, multimode...
Circulating tumor cells (CTCs) are early signs of metastasis and can be used to monitor disease progression well before radiological detection by imaging. Using an ultrasensitive graphene oxide microfluidic chip nanotechnology built with sheets, we were able demonstrate that CTCs specifically isolated molecularly characterized predict future in patients stage III non-small cell lung cancer (NSCLC). We analyzed from 26 at six time points throughout the treatment course chemoradiation followed...
Circulating tumor cells (CTCs) are extremely rare shed from tumors into the blood stream. These can provide valuable information about their of origin and direct treatment decisions to improve patient outcomes. Current technologies isolate CTCs a limited volume often require pre-processing that leads CTC loss, making it difficult enough perform in-depth analysis. Many inertial microfluidic devices have been developed at high flow rates, but they typically either dilution, remove red cells,...
Developing on‐site biomarker enrichment platforms could help to improve the diagnosis of gastrointestinal (GI) tract diseases at early stages. Medical procedures, such as colonoscopies and imaging techniques, are used diagnose a disease, but not easily accessible for repeat measurements. In contrast, liquid biopsies, e.g., blood, urine, or fecal samples, have become important sampling strategies identify health concerns. Herein, robotic pill is designed collecting relevant biomarkers from GI...
We present a simple strategy to immobilize and analyze extracellular vesicles for multiple markers on microfluidic device, called DICE.
The functional membrane proteins on tumor-cell-derived EVs contain a large amount of biomolecular information, and can serve as comprehensive marker to delineate the molecular nature cancer. However, due low secretion rates, it is difficult perform accurate quantification analysis with conventional EV technologies such Western blots. Here, we introduce multifunctional technology based an automated microfluidic chemiluminescent ELISA (Enzyme-Linked ImmunoSorbent Assay) platform. With this...
In non-small cell lung cancer (NSCLC), identifying the presence of sensitizing and resistance epidermal growth factor receptor (EGFR) mutations dictates treatment plans. Extracellular vesicles (EVs) are emerging as abundant, stable potential liquid biopsy targets that offer to quantify EGFR in NSCLC patients at RNA protein level multiple points through treatment. this study, we present a systematic approach for serial mutation profiling 34 EV samples from 10 metastatic with known Using...
Abstract This work presents chemically stable and biodegradable hydrogel beads for the isolation of circulating tumor cells (CTCs) exosomes in liquid biopsy. The biopsy ( LB beads) consisting alginate poly(vinyl alcohol) hydrogels show both chemical stability stimuli‐degradable characteristics. Unlike single‐component hydrogels, this hybrid form is not easily degraded by buffers or cell culture media while its degradable characteristic remains; thus, it useful bio‐applications requiring...
Abstract The recent push toward understanding an individual cell's behavior and identifying cellular heterogeneity has created unmet need for technologies that can probe live cells at the single‐cell level. Cells within a population are known to exhibit heterogeneous responses environmental cues. These differences lead varied states, behavior, therapeutics. Techniques needed not only capable of processing analyzing populations single cell level, but also have ability isolate specific from...
Glandular cancers are amongst the most prevalent types of cancer, which can develop in many different organs, presenting challenges their detection as well high treatment variability and failure rates. For that purpose, anticancer drugs commonly tested cancer cell lines grown 2D tissue culture on plastic dishesin vitro, or animal modelsin vivo. However, models diverge significantly from 3D characteristics living tissues require extensive use time. cancers, such prostate cancer-the second...
Abstract Small extracellular vesicles, often termed as “exosomes” carry informative cargo containing proteins and lipids, reflective of their cellular origin. Thus, they are promising biomarkers for early diagnosis cancer. However, conventional profiling methods like quantitative polymerase chain reaction (qPCR) require complex procedures, thereby limiting the analytical sensitivity exosomes liquid biopsies. Here, we demonstrate a sensitive microfluidic device (CDEXO) that isolates profiles...
Abstract Cancer-cell secreted small-extracellular vesicles, also known as exosomes, represent a rapidly emerging family of nanoscale biomarkers for cancer and other diseases. While being high in information content, the current protocols exosome profiling require complex procedures equipment involving purification, which prevents their broad utilization timely diagnosis. Here we show that chiral gold nanoparticles with twisted disk geometry can isolate profile cancer-associated directly from...
Abstract Introduction: Melanoma is among the most aggressive cancers, and its incidence continues to grow. Due lack of promising markers predict disease onset metastasis, early detection evaluation treatment efficacy have been hampered. Recent advances in liquid biopsy proposed alternatives for diagnosing with merits enabling continuous monitoring non-invasiveness. CTCs cancer exosomes are evolving as biomarkers due their innate capability having cancer-associated molecules signatures....
Introduction: Melanoma is among the most aggressive cancers, and its incidence continues to grow. Due lack of promising markers predict disease onset metastasis, early detection evaluation treatment efficacy have been hampered. Recent advances in liquid biopsy proposed alternatives for diagnosing with merits enabling continuous monitoring non-invasiveness. CTCs cancer exosomes are evolving as biomarkers due their innate capability having cancer-associated molecules signatures. However,...
Abstract Approximately 20% of non-small cell lung cancer (NSCLC) patients will be diagnosed with stage 3 cancer, a predicted survival rate between 20-30%. Stage NSCLC commonly undergo chemoradiation treatment, imaging scans being the standard care for determining tumor control or progression both during and after radiation treatment. Imaging technologies can only show it has occurred, making treatment challenging. We suggest that molecular characterization biomarkers found in bloodstream...
Abstract Introduction: Extracellular vesicles (EV), recovered from a liquid biopsy, are emerging as putative diagnostic test for cancer. To date, ultracentrifugation has been accepted the gold standard EV isolation despite its lengthy processing time and low recovery rate. Thanks to recent technological advances in microfluidics, several microfluidic devices have developed showing promise with respect improved rates, ability extract EVs small sample volumes, shorter compared...
Extracellular Vesicles and Circulating Nucleic Acids is an open access journal, focusing on extracellular vesicles circulating nucleic acids including DNA, RNA, miRNA their therapeutic use.
Developing on-site biomarker enrichment platforms could help to improve the diagnosis of gastrointestinal (GI) tract diseases at early stages. Medical procedures, such as colonoscopies and imaging techniques, are used diagnose disease, but not easily accessible for repeat measurements. In other hand, liquid biopsies, e.g. , blood, urine, or fecal samples, have become important sampling strategies identify health concerns. Herein, a robotic pill is designed collecting relevant biomarkers from...