A5026756467- Tuberculosis Research and Epidemiology
- Mycobacterium research and diagnosis
- Extracellular vesicles in disease
- Bacterial Infections and Vaccines
- RNA and protein synthesis mechanisms
- Caveolin-1 and cellular processes
- Bacterial Genetics and Biotechnology
- Antibiotic Resistance in Bacteria
- Infectious Diseases and Mycology
- Genomics and Phylogenetic Studies
- Infectious Diseases and Tuberculosis
- Galectins and Cancer Biology
- Amoebic Infections and Treatments
- HIV Research and Treatment
- Tumors and Oncological Cases
- Streptococcal Infections and Treatments
- Circular RNAs in diseases
- Inflammasome and immune disorders
- Drug Transport and Resistance Mechanisms
- Chromosomal and Genetic Variations
- Protein purification and stability
- Plant tissue culture and regeneration
- Microbial Community Ecology and Physiology
- Contact Dermatitis and Allergies
- Food Allergy and Anaphylaxis Research
New York State Department of Health
2022-2023
Rutgers, The State University of New Jersey
2018-2023
Wadsworth Center
2022-2023
Translational Health Science and Technology Institute
2018-2022
Rutgers New Jersey Medical School
2021
Duke University
2020
Bose Institute
1972-2020
Vanderbilt University
2020
Abstract Mycobacterium tuberculosis ( Mtb ) secretes extracellular vesicles (EVs) containing a variety of proteins, lipoproteins, and lipoglycans. While emerging evidence suggests that EVs contribute to pathogenesis, the factors molecular mechanisms involved in mycobacterial EV production have not been identified. In this study, we use genetic approach identify proteins mediate vesicle release response iron limitation antibiotic exposure. We uncover critical role for isoniazid‐induced,...
Coordinated bacterial cell septation and wall biosynthesis require formation of protein complexes at the sites division elongation, in a temporally controlled manner. The players these remain incompletely understood mycobacteria. Using vitro vivo assays, we showed that Rv2147c (or SepF) Mycobacterium tuberculosis interacts with principal driver cytokinesis, FtsZ. SepF also itself both vivo. Amino acid residues 189A, 190K 215F are required for FtsZ-SepF interaction, conserved across...
Toxin-antitoxin (TA) systems are bicistronic genetic modules that ubiquitously present in bacterial genomes. The Mycobacterium tuberculosis genome encodes 90 putative TA systems, and these considered to be associated with maintenance of genomic stability or survival under unfavorable environmental conditions. majority M. have been annotated as belonging the virulence-associated protein B C (VapBC) family. However, their precise role physiology has not elucidated. Here, we functionally...
The resuscitation-promoting factors of Mycobacterium tuberculosis are hydrolytic enzymes, which required for resuscitation dormant cells. RpfB, a peptidoglycan remodelling enzyme similar to the lytic transglycosylase Escherichia coli, is reactivation M. from chronic infection in vivo, underscoring need understand its transcriptional regulation. Here, we identified and translational start points rpfB, suggested rpf promoter-driven GFP expression vitro transcription assays that possibly occurs...
Mycobacterium abscessus is intrinsically resistant to antibiotics effective against other pathogenic mycobacteria largely due the drug-induced expression of genes that confer resistance. WhiB7 a major hub controlling induction resistance ribosome-targeting antibiotics. It activates >100 genes, 7 which are known determinants drug resistance; function most within regulon however unknown, but some conceivably encode additional mechanisms Furthermore, hierarchy gene regulon, if any, poorly...
ABSTRACT Mycobacterium tuberculosis (Mtb) secretes pathogenicity factors and immunologically active molecules via membrane vesicles. However, nothing is known about the mechanisms involved in mycobacterial vesicle biogenesis. This study investigates molecular determinants of production Mtb by analyzing cells under conditions high production: iron limitation VirR restriction. Ultrastructural analysis showed extensive cell envelope restructuring association with release that correlated...
Background: This study highlights the allergenicity and allergenic components of pollen Phoenix sylvestris Roxb. (PS), or date sugar palm, which is predominantly airborne in air Greater Calcutta. Methods: A 2‐year aerobiologic survey was performed by Burkard sampler. PS extract used skin tests allergic patients, fractionated (NH 4 ) 2 SO SephacrylS‐200 column. The each fraction checked test IgE ELISA inhibition. principal fraction, Fr.IIa, separated 11% SDS–PAGE, its confirmed inhibition...
The hydrocarbon-degrading bacterium Micrococcus luteus strain Modasa was isolated from contaminated soil Modasa, North Gujarat, India. Whole-genome sequencing and analysis provide an insight into the potentially important genes responsible for bioremediation.
Mycobacteria, including Mycobacterium tuberculosis (Mtb), the causative agent of human tuberculosis, naturally release extracellular vesicles (EVs) containing immunologically active molecules. Knowledge regarding molecular mechanisms vesicle biogenesis, content vesicles, and their functions at pathogen-host interface is very limited. Addressing these questions requires rigorous procedures for isolation, purification, validation EVs. Previously, production was found to be enhanced when M....
Mycobacteria, including Mycobacterium tuberculosis (Mtb), the causative agent of human tuberculosis, naturally release extracellular vesicles (EVs) containing immunologically active molecules. Knowledge regarding molecular mechanisms vesicle biogenesis, content vesicles, and their functions at pathogen-host interface is very limited. Addressing these questions requires rigorous procedures for isolation, purification, validation EVs. Previously, production was found to be enhanced when M....
Abstract Mycobacterium abscessus causes acute and chronic pulmonary infection in patients with lung damage. It is intrinsically resistance to antibiotics effective against other pathogenic mycobacteria largely due the drug-induced expression of genes that confer resistance. Induction upon exposure ribosome targeting proceeds via WhiB7-dependent -independent pathways. WhiB7 controls >100 genes, a few which are known determinants drug The function vast majority within regulon unknown, but...
Extracellular Vesicles and Circulating Nucleic Acids is an open access journal, focusing on extracellular vesicles circulating nucleic acids including DNA, RNA, miRNA their therapeutic use.