A5064615351- Influenza Virus Research Studies
- RNA and protein synthesis mechanisms
- interferon and immune responses
- Mosquito-borne diseases and control
- RNA modifications and cancer
- RNA Research and Splicing
- Vibrio bacteria research studies
- Viral Infections and Vectors
- Viral gastroenteritis research and epidemiology
- Immune Response and Inflammation
- Infectious Encephalopathies and Encephalitis
- Vector-borne infectious diseases
Harvard University
2024-2025
Boston VA Research Institute
2024-2025
University of Iowa
2022-2023
University of Chicago
2021-2023
Western equine encephalitis virus (WEEV) is an arbovirus that historically caused large outbreaks of throughout the Americas. WEEV binds protocadherin 10 (PCDH10) as a receptor, and highly virulent ancestral strains also bind low-density lipoprotein receptor (LDLR)-related proteins. As declined human pathogen in North America over past century, isolates have lost ability to mammalian receptors while still recognizing avian receptors. To explain shifts dependencies assess risk re-emergence,...
Western equine encephalitis virus (WEEV) is an arthropod-borne (arbovirus) that frequently caused major outbreaks of in humans and horses the early twentieth century, but frequency has since decreased markedly, strains this alphavirus isolated past two decades are less virulent mammals than 1930s 1940s
Abstract RNA molecules perform a diversity of essential functions for which their linear sequences must fold into higher-order structures. Techniques including crystallography and cryogenic electron microscopy have revealed 3D structures ribosomal, transfer, other well-structured RNAs; while chemical probing with sequencing facilitates secondary structure modeling any RNAs interest, even within cells. Ongoing efforts continue increasing the accuracy, resolution, ability to distinguish...
It is well documented that influenza A viruses selectively package 8 distinct viral ribonucleoprotein complexes (vRNPs) into each virion; however, the role of host factors in genome assembly not completely understood. To evaluate significance cellular assembly, we generated a reporter virus carrying tetracysteine tag NP gene (NP-Tc virus) and assessed dynamics vRNP localization with components by fluorescence microscopy. At early time points, were preferentially exported to MTOC;...
Abstract After decades of inactivity throughout the Americas, western equine encephalitis virus (WEEV) recently re-emerged in South America, causing a large-scale outbreak humans and horses. WEEV binds protocadherin 10 (PCDH10) as receptor; however, nonpathogenic strains no longer bind human or PCDH10 but retain ability to avian receptors. Highly virulent can also very low-density lipoprotein receptor (VLDLR) apolipoprotein E 2 (ApoER2) alternative Here, by determining cryo-electron...
<title>Abstract</title> RNA molecules perform a diversity of essential functions for which their linear sequences must fold into higher-order structures. Techniques including crystallography and cryogenic electron microscopy have revealed 3D structures ribosomal, transfer, other well-structured RNAs; while chemical probing with sequencing facilitates secondary structure modeling any RNAs interest, even within cells. Ongoing efforts continue increasing the accuracy, resolution, ability to...
Summary It is well documented that influenza A viruses selectively package 8 distinct viral ribonucleoprotein complexes (vRNPs) into each virion; however, the role of host factors in genome assembly not completely understood. To evaluate significance cellular assembly, we generated a reporter virus carrying tetracysteine tag NP gene (NP-Tc virus) and assessed dynamics vRNP localization with components by fluorescence microscopy. At early time points, were preferentially exported to MTOC;...
It is well known that influenza A viruses (IAV) initiate host cell infection by binding to sialic acid, a sugar molecule present at the ends of various chains called glycoconjugates. These can vary in chain length, structure, and composition. However, it remains unknown if IAV strains preferentially bind acid on specific glycoconjugate type(s) for infection. Here, we utilized CRISPR gene editing abolish different types human lung cells, evaluated versus avian infections. Our studies show...
Abstract IAV utilize sialic acid (Sia) containing cell surface glycoconjugates for host infection, and strains from different species show preferences structurally distinct Sia at the termini of glycoconjugates. Various types (N-glycans, O-glycans, glycolipids) display significant diversity in both structure carbohydrate composition. To define that facilitate we utilized CRISPR/Cas9 technique to truncate glycoconjugates, either individually or combination, by targeting glycosyltransferases...