A5059337154- Salmonella and Campylobacter epidemiology
- Viral gastroenteritis research and epidemiology
- Vibrio bacteria research studies
- Helicobacter pylori-related gastroenterology studies
- Yeasts and Rust Fungi Studies
- Plant Pathogens and Fungal Diseases
- Cystic Fibrosis Research Advances
- Bacterial Genetics and Biotechnology
- Veterinary medicine and infectious diseases
- RNA and protein synthesis mechanisms
- Escherichia coli research studies
- Fermentation and Sensory Analysis
- Galectins and Cancer Biology
- Listeria monocytogenes in Food Safety
- Legume Nitrogen Fixing Symbiosis
- Bacteriophages and microbial interactions
- Bacterial biofilms and quorum sensing
- Bone Tissue Engineering Materials
- Eosinophilic Esophagitis
- Antibiotic Resistance in Bacteria
- Electrostatics and Colloid Interactions
- Animal Genetics and Reproduction
- Gastroesophageal reflux and treatments
- Dental materials and restorations
- Bacterial Infections and Vaccines
University College Dublin
2012-2025
Our Lady's Hospital
1998-2016
Children's Health Ireland at Crumlin
1998-2016
National Children’s Research Centre
2016
Trinity College Dublin
2006-2011
Molecular Medicine Ireland
2007
Center for Biologics Evaluation and Research
2005
Vrije Universiteit Amsterdam
2000
Tohoku University
2000
Summary The integration host factor (IHF) is a DNA‐binding and ‐bending protein with roles in local DNA structural organization transcriptional regulation Gram‐negative bacteria. This heterodimeric composed of the two highly homologous subunits IHFα IHFβ. microarray analysis was used to define regulon genes subject IHF control Salmonella enterica serovar Typhimurium ( S. Typhimurium). transcription profile wild type compared those mutants deficient IHFα, IHFβ, or both Our data reveal new...
The role of the HU nucleoid-associated proteins in gene regulation was examined Salmonella enterica serovar Typhimurium. dimeric protein consists different combinations its α and β subunits. Transcriptomic analysis performed with cultures growing at 37 °C 1, 4 6 h after inoculation mutants that lack . Distinct but overlapping patterns expression were detected each time point for three mutants, revealing not one regulons genes controlled by proteins. Mutations hup altered regulatory...
Summary Adaptation of bacterial pathogens to an intracellular environment requires resetting the expression levels a wide range both virulence and housekeeping genes. We investigated possibility that changes in DNA supercoiling could modulate genes known be important growth pathogen Salmonella enterica serovar Typhimurium. Our data show becomes relaxed when grows murine macrophage but not epithelial cells, indicating plays role discrimination between two types environment. The ssrA...
Summary The classic expression pattern of the Fis global regulatory protein during batch culture consists a high peak in early logarithmic phase growth, followed by sharp decrease through mid‐exponential growth until is almost undetectable at end exponential phase. We discovered that this contingent on regime. In Salmonella enterica serovar Typhimurium cultures grown non‐aerated SPI1‐inducing conditions, can be detected readily stationary On other hand, with standard aeration showed pattern....
Abstract The World Health Organization has listed C . jejuni as one of 12 microorganisms on a global priority list for antibiotic resistance due to rapid increase in strains resistant fluoroquinolone antibiotics. This is conferred through single point mutation the QRDR region within gyrA gene known be involved DNA supercoiling. We have previously revealed that changes supercoilikng play major role regulation virulence with relaxation supercoiling associated increased attachment and invasion...
Abstract Lager brewing first occurred in Bavaria the 15th century, associated with restrictions of to colder months. The lager yeast, Saccharomyces pastorianus, is cold tolerant. It a hybrid between cerevisiae and eubayanus, has been found only industrial settings. Natural isolates S. eubayanus were discovered Patagonia 11 years ago. They have since isolated from China, Tibet, New Zealand, North America, but not Europe. Here, we describe European strains UCD646 UCD650, wooded area on...
Site-specific recombinases of the integrase family usually require cofactors to impart directionality in recombination reactions that they catalyze. The FimB inverts Escherichia coli fim switch (fimS) on-to-off and off-to-on directions with approximately equal efficiency. Inhibiting DNA gyrase novobiocin caused inversion become biased direction. This was not due differential topological distortion fimS on off phases by activity its resident P(fimA) promoter. Instead, leucine-responsive...
Implants made from titanium are used as prostheses because of their biocompatibility and mechanical properties close to those human bone. However, the risk bacterial infection is always a major concern during surgery, development biofilm can make these infections difficult treat. A promising strategy mitigate against use antifouling antimicrobial coatings, where bioresorbable polymers play an important role due controlled degradability sustained drug release, well excellent biocompatibility....
ABSTRACT Candida solani is a member of the Wickerhamomyces clade budding yeasts. We present genome sequence C. strain UCD1087, which was isolated from soil on University College Dublin (UCD) campus in Ireland. This 12.85 Mb and assembled into six chromosome-sized contigs plus mitochondrial contig.
ABSTRACT Isogenic flagellum-negative mutants of Helicobacter pylori and mustelae were screened for their ability to adhere primary human ferret gastric epithelial cells, respectively. We also evaluated the adherence an H. strain with a mutation in flbA gene, homologue flbF/lcrD family genes known be involved regulation flagellar biosynthesis. deficient production FlaA or FlaB both showed no reduction cells compared wild-type parental strains. However, mutant was significantly reduced strain....
Campylobacter jejuni is the leading cause of bacterial gastroenteritis in humans, but relatively little known about global regulation virulence factors during infection chickens or humans. This study identified DNA supercoiling as playing a key role regulating motility and flagellar protein production found that this supercoiling-controlled regulon induced by growth chicken mucus. A direct correlation was observed between resting levels different strains C. jejuni, relaxation resulted...
Summary Invasion of intestinal epithelial cells by Campylobacter jejuni is a critical step during infection the intestine this important human pathogen. In study we investigated role played DNA supercoiling in regulation invasion and mechanism which could be mediated. A significant correlation between more relaxed an increased ability C. strains to penetrate was demonstrated. Directly inducing relaxation shown significantly increase cells. Mutants fibronectin binding proteins CadF FlpA still...
ABSTRACT Infection with Helicobacter pylori has been associated induction of autoantibodies that cross-react the gastric mucosa. There have discordant reports as to whether or not these arise due molecular mimicry between H. and host cell antigens on parietal cells. In this study, we investigated by mustelae causes in infected ferrets. Serum from -infected ferrets reacted cells ferret mucosa but duodenal colonic These sera did react blood group A epitope erythrocytes lipopolysaccharide,...
The BvgAS two-component system positively regulates the expression of virulence genes Bordetella pertussis and negatively a second set whose function is unknown. BvgAS-mediated regulation bvg-repressed accomplished through activation negative regulator, BvgR. A regulatory system, RisAS, required for surface antigens VraA VraB. We examined roles BvgR RisA in four B. pertussis. Our analyses demonstrated that all are repressed by product bvgR locus activated risA locus. Deletion analysis vrg6...
Helicobacter mustelae is a gastric pathogen of ferrets, where it causes disorders similar to those caused by pylori in humans. The H. ferret model therefore has potential for the vivo study pathogenesis general. In this library 500 individual mutants was generated using an vitro random insertion mutagenesis technique. Mutants were subsequently tested motility and adherence, 43 found be nonmotile soft agar assay. Of these mutants, seven identified as deficient their ability adhere AGS cells....
We sequenced two isolates of Kazachstania servazzii, UCD13 and UCD335, from soil in Ireland. Heterozygosity these diploid genomes differs 19-fold between the strains. Most currently available K. servazzii genome sequences come Korean kimchi isolates, so our data will facilitate analysis diversity this species.
Campylobacter jejuni is the leading cause of bacterial gastroenteritis worldwide and main source infection contaminated chicken meat. Although this important human pathogen an obligate microaerophile, it must survive atmospheric oxygen conditions to allow transmission from meat humans. It becoming increasingly evident that formation biofilm plays a key role in survival organism for extended periods on poultry products. We have recently demonstrated novel inducible model study adherent C....
Abstract Adaptation of opportunistic pathogens to their host environment requires reprogramming a vast array genes facilitate survival in the host. Burkholderia cenocepacia , Gram‐negative bacterium with large genome ∼8 Mb that colonizes environmental niches, is exquisitely adaptable hypoxic cystic fibrosis lung and survives macrophages. We previously identified an immunoreactive acidic protein encoded on replicon 3, BCAS0292. Deletion BCAS0292 gene significantly altered abundance 979...
Campylobacter species are the leading cause of bacterial diarrhoea worldwide and consumption contaminated chicken meat is most common route infection. Chickens can be infected with multiple strains during infection cycle this pathogen must survive a wide variety environments. Numerous studies have reported high degree genetic variability in that use antigenic phase variation to alter expression key phenotypes. In study phenotypic profile isolates from freshly slaughtered chickens, products...