A5039365156- Mass Spectrometry Techniques and Applications
- Analytical Chemistry and Chromatography
- Analytical chemistry methods development
- Photoreceptor and optogenetics research
- Bacterial biofilms and quorum sensing
- Photosynthetic Processes and Mechanisms
- Metabolomics and Mass Spectrometry Studies
- Light effects on plants
- Advanced Chemical Sensor Technologies
- Microfluidic and Capillary Electrophoresis Applications
- Plasma Applications and Diagnostics
- Probiotics and Fermented Foods
- Pesticide Residue Analysis and Safety
University of Freiburg
2022
University of Duisburg-Essen
2017-2021
High-throughput liquid chromatography/mass spectrometry (LC/MS) is an increasing topic in analytical chemistry. Especially the idle time of a mass spectrometer should be reduced for efficient and cost-saving use. Therefore, fast-switching dual ion source was developed, which uses most important ionization techniques at atmospheric pressure, electrospray (ESI) pressure chemical (APCI), with one or more LC systems.The performance developed shown by infusion experiments chromatographic analyses...
Abstract Cystic fibrosis (CF) is an autosomal recessive inherited disease which leads to a production of thickened mucus in the airways. These conditions are conducive poly-microbial infections, like chronic lung infection, Pseudomonas aeruginosa ( P. ) major pathogenic bacterium colonizing CF lungs at end lifetime patients. This vitro study uses biofilm model under partly cystic conditions, with sampling volatile extracellular metabolites. The gas was done thin-film microextraction (TFME)...
Rationale An argon inverse low‐temperature plasma (iLTP) ionization source for liquid chromatography/tandem mass spectrometry was developed. The iLTP is constructed from simple chromatographic supply materials and implemented into an atmospheric pressure chemical (APCI) replacing the APCI discharge needle electrode. newly developed ion coupled to ultra‐high‐performance chromatography (UHPLC) system. Methods characterized by optical emission spectroscopy. soft of selected standards also...
The optogenetic tool LEXY consists of the second light oxygen voltage (LOV) domain Avena sativa phototropin 1 mutated to contain a nuclear export signal. It allows exporting from nucleus with blue proteins interest (POIs) genetically fused it. Mutations slowing dark recovery rate LOV within were recently shown allow for better depletion some POIs in Drosophila embryos and usage low illumination regimes. We investigated these variants mammalian cells found they increase cytoplasmic...