A5064603989- Coral and Marine Ecosystems Studies
- Entomopathogenic Microorganisms in Pest Control
- Insect symbiosis and bacterial influences
- Insect Resistance and Genetics
- Microbial Community Ecology and Physiology
- Marine Sponges and Natural Products
- Marine and coastal plant biology
- Insect and Pesticide Research
- Coastal wetland ecosystem dynamics
- Marine Invertebrate Physiology and Ecology
- Aquaculture disease management and microbiota
- Genomics and Phylogenetic Studies
- Insect and Arachnid Ecology and Behavior
- Cephalopods and Marine Biology
- Invertebrate Immune Response Mechanisms
- Geological and Geophysical Studies
- Insect Utilization and Effects
- Transgenic Plants and Applications
- Paleontology and Stratigraphy of Fossils
- Forensic Entomology and Diptera Studies
- Protist diversity and phylogeny
- Environmental DNA in Biodiversity Studies
- Insect Pest Control Strategies
- Insects and Parasite Interactions
- Nematode management and characterization studies
The University of Melbourne
2020-2025
Biologie Fonctionnelle Insectes et Interactions
2016-2024
Université Claude Bernard Lyon 1
2019-2023
Institut National des Sciences Appliquées de Lyon
2019-2023
Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement
2020-2023
Centre National des Sciences et Technologies Nucléaires
2021
Manouba University
2021
Corals are associated with a variety of bacteria, which occur in the surface mucus layer, gastrovascular cavity, skeleton, and tissues. Some tissue-associated bacteria form clusters, termed cell-associated microbial aggregates (CAMAs), poorly studied. Here, we provide comprehensive characterization CAMAs coral Pocillopora acuta. Combining imaging techniques, laser capture microdissection, amplicon metagenome sequencing, show that (i) located tentacle tips may be intracellular; (ii) contain...
Corals house a variety of microorganisms which they depend on for their survival, including endosymbiotic dinoflagellates (Symbiodiniaceae) and bacteria. While cnidarian-microorganism interactions are widely studied, Symbiodiniaceae-bacteria only just beginning to receive attention. Here, we describe the localization composition bacterial communities associated with cultures 11 Symbiodiniaceae strains from nine species six genera. Three-dimensional confocal laser scanning electron microscopy...
Bacterial intracellular symbiosis (endosymbiosis) is widespread in nature and impacts many biological processes. In holometabolous symbiotic insects, metamorphosis entails a complete abrupt internal reorganization that creates constraint for endosymbiont transmission from larvae to adults. To assess how endosymbiosis copes-and potentially evolves-throughout this major host-tissue reorganization, we used the association between cereal weevil Sitophilus oryzae bacterium Sodalis pierantonius as...
The rice weevil Sitophilus oryzae is one of the most important agricultural pests, causing extensive damage to cereal in fields and stored grains. S. has an intracellular symbiotic relationship (endosymbiosis) with Gram-negative bacterium Sodalis pierantonius a valuable model decipher host-symbiont molecular interactions.We sequenced genome using combination short long reads produce best assembly for Curculionidae species date. We show that undergone successive bursts transposable element...
Many insects developing on nutritionally unbalanced diets have evolved symbiotic associations with vertically transmitted intracellular bacteria (endosymbionts) that provide them metabolic components, thereby improving the host's abilities to thrive such poor ecological niches. While host-endosymbiont coevolutionary constraints are known entail massive genomic changes in microbial partner, evolution remains elusive, particularly regard immune system. In cereal weevil Sitophilus spp., which...
Long-term intracellular symbiosis (or endosymbiosis) is widely distributed across invertebrates and recognized as a major driving force in evolution. However, the maintenance of immune homeostasis organisms chronically infected with mutualistic bacteria challenging task, little known about molecular processes that limit endosymbiont immunogenicity host inflammation. Here, we investigated peptidoglycan recognition protein (PGRP)-encoding genes cereal weevil Sitophilus zeamais ’s association...
Abstract To survive in nutrient-poor waters corals rely on a symbiotic association with intracellular microalgae. However, increased sea temperatures cause algal loss—known as coral bleaching—often followed by death. Some of the most compelling evidence support ‘oxidative stress theory bleaching’ comes from studies that exposed corals, cultures their endosymbionts, or model Exaiptasia diaphana to exogenous antioxidants during thermal stress. Here, we replicate these experiments using E....
Coral reefs house one-third of all marine species and are high cultural socioeconomic importance. However, coral under dire threat from climate change other anthropogenic stressors. Climate is causing bleaching, the breakdown symbiosis between host its algal symbionts, often resulting in mortality deterioration these valuable ecosystems. While it essential to counteract root causes change, remains urgent develop restoration conservation methods that will buy time for reefs. The manipulation...
Abstract Coral microhabitats are colonized by a myriad of microorganisms, including diverse bacteria which essential for host functioning and survival. However, the location, transmission, functions individual bacterial species living inside coral tissues remain poorly studied. Here, we show that previously undescribed symbiont Pocillopora acuta forms cell-associated microbial aggregates (CAMAs) within mesenterial filaments. CAMAs were found in both adults larval offspring, suggesting...
Coral reefs are threatened by climate change, because it causes increasingly frequent and severe summer heatwaves, resulting in mass coral bleaching mortality. is believed to be driven an excess production of reactive oxygen (ROS) nitrogen species (RNS), yet their relative roles during thermal stress remain understudied. Here, we measured ROS RNS net production, as well activities key enzymes involved scavenging (superoxide dismutase catalase) synthesis (nitric oxide synthase) linked these...
Heat-tolerant strains of the coral endosymbiont, Cladocopium C1acro (Symbiodiniaceae), have previously been developed via experimental evolution. Here, we examine physiological responses and bacterial community composition (using 16S rRNA gene metabarcoding) in cultures 10 heat-evolved (SS) 9 wild-type (WT) strains, which had exposed for 6 years to 31 °C 27 °C, respectively. We also whether associated communities were affected by a three-week reciprocal transplantation both temperatures. The...
Coral reefs are extremely vulnerable to ocean warming, which triggers coral bleaching-the loss of endosymbiotic microalgae (Symbiodiniaceae) from tissues, often leading death. To enhance climate resilience, the symbiont, Cladocopium proliferum was experimentally evolved for >10 years under elevated temperatures resulting in increased heat tolerance. Bacterial 16S rRNA gene metabarcoding showed composition intra- and extracellular bacterial communities heat-evolved strains significantly...
Abstract Background Coral reefs are among the most diverse and productive ecosystems on Earth. This success relies coral’s association with a wide range of microorganisms, including dinoflagellates family Symbiodiniaceae that provide coral hosts their organic carbon requirements. While bacterial associates have long been overlooked, research these microorganisms is gaining traction, deciphering identity function greatly enhancing our understanding cnidarian biology. Here, we investigated...
Symbiotic microorganisms are crucial for the survival of corals and their resistance to coral bleaching in face climate change. However, impact microbe-microbe interactions on functioning is mostly unknown but could be essential factors adaption future climates. Here, we investigated between cultured dinoflagellates Symbiodiniaceae family, photosymbionts corals, associated bacteria. By assessing genomic potential 49 bacteria, found that they likely beneficial Symbiodiniaceae, through...
The bacterial genus Endozoicomonas is a predominant member of the coral microbiome, widely recognised for its ubiquity and ability to form high-density aggregates within tissues. Hence, investigating metabolic interplay with hosts offers critical insights into ecological roles contributions health resilience. Using long- short-read whole-genome sequencing 11 strains from Acropora loripes , genome sizes were found range between 5.8 7.1 Mbp. Phylogenomic analysis identified two distinct clades...
Coral reefs are diverse marine ecosystems that have tremendous ecological and cultural value support more than 25% of eukaryote biodiversity. Increased ocean temperatures light intensity trigger coral bleaching, the breakdown relationship between corals their photosymbionts, dinoflagellates family Symbiodiniaceae. This leaves without primary energy source, thereby leading to starvation and, often, death. bleaching is hypothesized occur due an overproduction reactive oxygen species (ROS) by...
Abstract Cnidarians, such as corals and sea anemones, associate with a wide range of bacteria that have essential functions, including nutrient cycling the production antimicrobial compounds. Within cnidarians, can colonize all microhabitats tissues. Among them are obligate intracellular phylum Chlamydiota (chlamydiae) whose impact on cnidarian hosts holobionts, especially corals, remain unknown. Here, we conducted meta-analysis previously published 16S rRNA gene metabarcoding data from...
Chlamydiae are ubiquitous intracellular bacteria and infect a wide diversity of eukaryotes, including mammals. However, chlamydiae have never been reported to photosynthetic organisms. Here, we describe novel chlamydial genus species, Candidatus Algichlamydia australiensis, capable infecting the dinoflagellate Cladocopium sp. (originally isolated from scleractinian coral). australiensis was confirmed be by fluorescence in situ hybridization confocal laser scanning microscopy temporally...
Bacteria in the genus Endozoicomonas are well-known coral symbionts commonly found as clusters within tissues of several species. Mapping spatial distribution these microbial communities is critical to gaining a holistic understanding potential role they may play host. This study focuses on characterising bacterial aggregates associated with common reef-building coral, Acropora loripes , from central Great Barrier Reef, Australia. A conventional cultivation-based method was employed...
ABSTRACT Virtually all animals associate with beneficial symbiotic bacteria. Whether and how these associations are modulated across a host's lifecycle is an important question in disentangling animal-bacteria interactions. We recently reported case of complete morphological reorganization symbiosis during metamorphosis the cereal weevil, Sitophilus oryzae. In this model, bacteriome, specialized organ that houses intracellular bacterium Sodalis pierantonius, undergoes two-phase remodeling...