A5010656428- Advanced MRI Techniques and Applications
- Electron Spin Resonance Studies
- Lanthanide and Transition Metal Complexes
- MRI in cancer diagnosis
- Multiple Sclerosis Research Studies
- Ultrasound and Hyperthermia Applications
- Systemic Lupus Erythematosus Research
- Atomic and Subatomic Physics Research
- COVID-19 Digital Contact Tracing
- COVID-19 epidemiological studies
- Medical Imaging Techniques and Applications
- CNS Lymphoma Diagnosis and Treatment
- Privacy-Preserving Technologies in Data
- Autoimmune and Inflammatory Disorders Research
- Advanced Neuroimaging Techniques and Applications
- Disaster Response and Management
- Renal Diseases and Glomerulopathies
- Advanced X-ray and CT Imaging
- COVID-19 Impact on Reproduction
- Kawasaki Disease and Coronary Complications
- Vasculitis and related conditions
- Tracheal and airway disorders
- Ethics and Social Impacts of AI
- Advanced NMR Techniques and Applications
- Cytomegalovirus and herpesvirus research
Philippine General Hospital
2025
Max Delbrück Center
2017-2021
Charité - Universitätsmedizin Berlin
2020-2021
Scientific Research Institute of Experimental and Clinical Medicine
2021
Hospital Italiano de Buenos Aires
2021
Hospital Universitario de Burgos
2020
Neuroinflammation can be monitored using fluorine-19 (19F)-containing nanoparticles and 19F MRI. Previously we studied neuroinflammation in experimental autoimmune encephalomyelitis (EAE) room temperature (RT) radiofrequency (RF) coils low spatial resolution MRI to overcome constraints signal-to-noise ratio (SNR). This yielded an approximate localization of inflammatory lesions. Here used a new transceive cryogenic quadrature RF probe ( 19 F-CRP) that provides the SNR necessary acquire...
The use of rigid multi-exponential models (with a priori predefined numbers components) is common practice for diffusion-weighted MRI (DWI) analysis the kidney. This approach may not accurately reflect renal microstructure, as data are forced to conform assumptions simplified models. work examines feasibility less constrained, data-driven non-negative least squares (NNLS) continuum modelling DWI kidney tubule system in simulations that include emulations pathophysiological...
The brain ventricles are part of the fluid compartments bridging CNS with periphery. Using MRI, we previously observed a pronounced increase in ventricle volume (VV) experimental autoimmune encephalomyelitis (EAE) model multiple sclerosis (MS). Here, examined VV changes EAE and MS patients longitudinal studies frequent serial MRI scans. mice underwent for up to 2 months, gadolinium contrast as proxy inflammation, confirmed by histopathology. We performed time-series analysis clinical data...
Fluorine MR would benefit greatly from enhancements in signal-to-noise ratio (SNR). This study examines the sensitivity gain of 19F that can be practically achieved when moving 9.4 to 21.1 T.We studied perfluoro-15-crown-5-ether (PFCE) at both field strengths (B0), as a pure compound, form nanoparticles (NP) employed inflammation vivo, well inflamed tissue. Brains, lymph nodes (LNs) and spleens were obtained mice with experimental autoimmune encephalomyelitis (EAE) had been administered PFCE...
Background: Magnetic resonance imaging (MRI) is indispensable for diagnosing neurological conditions such as multiple sclerosis (MS).MRI also supports decisions regarding the choice of disease-modifying drugs (DMDs).Determining in vivo tissue concentrations DMDs has potential to become an essential clinical tool therapeutic drug monitoring (TDM).The aim here was examine feasibility fluorine-19 ( 19 F) MR methods detect fluorinated DMD teriflunomide (TF) during normal and pathological...
Fluorine (19F) magnetic resonance imaging (MRI) is severely limited by a low signal-to noise ratio (SNR), and tapping it for 19F drug detection in vivo still poses significant challenge. However, bears the potential label-free theranostic imaging. Recently, we detected fluorinated dihydroorotate dehydrogenase (DHODH) inhibitor teriflunomide (TF) noninvasively an animal model of multiple sclerosis (MS) using MR spectroscopy (MRS). In present study, probed distinct modifications to CF3 group...
Abstract Kidney-associated pathologies would greatly benefit from noninvasive and robust methods that can objectively quantify changes in renal function. In the past years there has been a growing incentive to develop new applications for fluorine ( 19 F) MRI biomedical research study functional during disease states. F represents an instrumental tool quantification of exogenous substances vivo. One major benefits is its organic form absent eukaryotic cells. Therefore, introduction signals...
The use of surface radiofrequency (RF) coils is common practice to boost sensitivity in (pre)clinical MRI. number transceive RF rapidly growing due the surge cryogenically cooled technology and ultrahigh-field Consequently, there an increasing need for effective correction excitation field ( B1+ ) inhomogeneity inherent these coils. Retrospective B1 permits quantitative MRI, but this usually requires a pulse sequence-specific analytical signal intensity (SI) equation. Such equation not...
Purpose Low SNR in fluorine‐19 ( 19 F) MRI benefits from cryogenically‐cooled transceive surface RF probes (CRPs), but strong B 1 inhomogeneities hinder quantification. Rapid acquisition with refocused echoes (RARE) is an SNR‐efficient method for of neuroinflammation perfluorinated compounds lacks analytical signal intensity equation to retrospectively correct inhomogeneity. Here, a workflow was proposed and validated quantify F‐MR signals the inflamed mouse brain using F‐CRP. Methods In...
Abstract Magnetic resonance imaging (MRI) is a noninvasive technology that offers unparalleled anatomical and functional detail, along with diagnostic sensitivity. MRI suitable for longitudinal studies due to the lack of exposure ionizing radiation. Before undertaking preclinical investigations kidney, appropriate hardware should be carefully chosen balance competing demands image quality, spatial resolution, speed, tailored specific scientific objectives investigation. Here we describe...
Abstract Here we describe a simple and inexpensive protocol for preparing ex vivo rodent phantoms use in MR imaging studies. The experimental animals are perfused fixed with formaldehyde, then wrapped gauze sealed liquid latex. This yields phantom that preserves all organs situ, which avoids the need to keep containers have dimensions very different from living animals. is especially important loading detectors, specifically RF coils, they usually used with. can be safely stored conveniently...
The global SARS-CoV-2 pandemic is currently putting a massive strain on the world's critical infrastructures. With healthcare systems and internet service providers already struggling to provide reliable service, some operators may, intentionally or unintentionally, lever out privacy-protecting measures increase their system's efficiency in fighting virus. Moreover, though it may seem all encouraging see effectiveness of authoritarian states battling crisis, we, authors this paper, would...
Abstract Inflammation is one underlying contributing factor in the pathology of acute and chronic kidney disorders. Phagocytes such as monocytes, neutrophils dendritic cells are considered to play a deleterious role progression disease but may also contribute organ homeostasis. The target life-threatening autoimmune disorders antineutrophil cytoplasmic antibody (ANCA)-associated vasculitides (AAV). Neutrophils monocytes express ANCA antigens an important pathogenesis AAV. Noninvasive vivo...
Improving sensitivity has been the major challenge towards realizing promises of 19 F-MRI. Here we combine a cryogenic surface RF probe and compressed sensing to achieve high spatially resolved F-MRI neuroinflammation in challenging longitudinal study. Inflammation CNS was observed even absence neurological symptoms detailed signals along vasculature are clearly resolved. Together with effective registration this allows an in-depth study disease progression.
Modifications to the CF 3 side-group of teriflunomide were made improve its detection by 19 F MRI. Pentafluorosulfanyl (SF 5 ) was shown be a superior alternative . The SNR efficiency three MRI methods showed that within biological environment, SF -substitutions gave highest in combination with an ultrashort echo-time (UTE) method. Chemical modifications did not hamper activity but -TF more effective inhibit T cell proliferation, indicating better anti-inflammatory activity. This study...