A5081077592- Adipose Tissue and Metabolism
- Epigenetics and DNA Methylation
- Genomics and Chromatin Dynamics
- Peroxisome Proliferator-Activated Receptors
- Sirtuins and Resveratrol in Medicine
- Ubiquitin and proteasome pathways
- Histone Deacetylase Inhibitors Research
- CRISPR and Genetic Engineering
- Adipokines, Inflammation, and Metabolic Diseases
- Lipid metabolism and biosynthesis
- Wheat and Barley Genetics and Pathology
- RNA modifications and cancer
- Mycotoxins in Agriculture and Food
- Cholesterol and Lipid Metabolism
- Cancer-related gene regulation
- Animal Genetics and Reproduction
- RNA and protein synthesis mechanisms
- Gene expression and cancer classification
- Biochemical effects in animals
- Muscle Physiology and Disorders
- Photonic and Optical Devices
- Genomics and Phylogenetic Studies
- Metabolism, Diabetes, and Cancer
- RNA Research and Splicing
- Plant Molecular Biology Research
Agency for Science, Technology and Research
2016-2025
Institute of Molecular and Cell Biology
2016-2025
Merchants Chongqing Communications Research and Design Institute
2024
National University of Singapore
2013-2024
Xiyuan Hospital
2019-2024
Chinese Academy of Medical Sciences & Peking Union Medical College
2019-2024
New York University
2024
Southern Medical University
2021-2024
Institute of Microelectronics
2023-2024
Fudan University Shanghai Cancer Center
2024
Polycomb group proteins and the related histone modification H3K27me3 can maintain silencing of key developmental regulators provide cellular memory. However, how such an epigenetic state is reprogrammed inherited between generations poorly understood. Using ultra-sensitive approach, STAR ChIP-seq, we investigated across 14 stages along mouse gametogenesis early development. Interestingly, highly pervasive found in regions depleted transcription DNA methylation oocytes. Unexpectedly,...
Histone variants and their post-translational modifications help regulate chromosomal functions. Htz1 is an evolutionarily conserved H2A variant found at several promoters in the yeast Saccharomyces cerevisiae . In this study, we undertook a genome-wide analysis of its yeast. Using mass spectrometric analysis, determined that acetylated Lys 3, 8, 10, 14 within N-terminal tail, with K14 being most abundant site. ChIP microarray were then used to compare location Htz1-K14 acetylation...
We report the design and synthesis of a specific mitochondrial fluorescent probe <bold>AIE-MitoGreen-1</bold> with AIE characteristics to monitor morphology changes identify differentiation process living brown adipose cells.
The N6-methyladenosine binding protein YTHDF1, often upregulated in cancer, promotes tumor growth and hinders immune checkpoint blockade treatment. A comprehensive understanding of the molecular mechanisms governing YTHDF1 stability is pivotal for enhancing clinical response rates effectiveness cancer patients. Here, we report that USP5 interacts with stabilizing it by removing K11-linked polyubiquitination. Insulin activates mTORC1, phosphorylating promoting its dimerization, which binds to...
Brown adipose tissue (BAT) activation and subcutaneous white fat browning are essential components of the thermogenic response to cold stimulus in mammals. microRNAs have been shown regulate both processes cis. Here, we identify miR-32 as a BAT-specific super-enhancer-associated miRNA mice that is selectively expressed BAT further upregulated during exposure. Inhibiting vivo led impaired tolerance, decreased thermogenesis, compromised result reduced serum FGF21 levels. Further examination...
Obesity is a critical risk factor for hepatocellular carcinoma (HCC). However, it remains unknown whether inhibition of de novo lipid biosynthesis can suppress HCC. In this study, we blocked the sterol regulatory element‐binding protein (SREBP) pathway, one key determinants homeostasis, by ablating 78‐kDa cell‐surface glycoprotein or SREBP cleavage‐activating in hepatocytes, as well administering chemical compound called betulin. We found that either genetically pharmacologically inhibiting...
Histone modifications and their modifying enzymes are fundamentally involved in the epigenetic regulation of adipogenesis. This study aimed to define roles various histone "division labor" fat cell differentiation. To achieve these goals, we examined distribution patterns eight core at five key adipogenic regulatory genes, Pref-1, C/EBPβ, C/EBPα, PPARγ2 aP2, during adipogenesis C3H 10T1/2 mouse mesenchymal stem cells (MSCs) 3T3-L1 preadipocytes. We found that globally stable throughout but...
Seipin regulates lipid homeostasis by preventing droplet (LD) formation in non-adipocytes but promoting it developing adipocytes. Here, we report that seipin interacts with 14-3-3β through its N- and C-termini. Expression of is upregulated during adipogenesis, deletion results defective adipogenesis without affecting key adipogenic transcription factors. We further identified the actin-severing protein cofilin-1 as an interacting partner to 14-3-3β. Cofilin-1 was spatiotemporally recruited...
Ovulation rate and litter size are important reproductive traits in sheep with high economic value. Recent work has revealed a potential link between DNA methylation prolificacy. However, genome-wide study that sought to identify sites involved prolificacy indicated it is still unknown. Here, we aimed investigate the profiles of Hu ovaries by comparing high-prolificacy group (HP, three for at least 2 consecutive lambings) low (LP, one using deep whole-genome bisulfite sequencing (WGBS)....
Individuals who are born small for gestational age (SGA) have a risk to develop various metabolic diseases during their life course. The biological memory of the prenatal state growth restricted individuals may be reflected in epigenetic alterations stem cell populations. Mesenchymal cells (MSCs) from Wharton's jelly umbilical cord tissue multipotent, and we generated primary MSC isolates SGA normal neonates, which were subsequently differentiated into adipocytes. We established chromatin...
Increasing energy expenditure through brown adipocyte recruitment is a promising approach to combat obesity. We report here the comprehensive profiling of epigenome and transcriptome throughout lineage commitment differentiation C3H10T1/2 mesenchymal stem cell line into adipocytes. Through direct comparison datasets from differentiating white adipocytes, we systematically identify stage- lineage-specific coding genes, lncRNAs microRNAs. Utilizing chromatin state maps, also define enhancers,...
Cytoskeleton remodelling is a prerequisite step for the morphological transition from preadipocytes to mature adipocytes. Although microtubules play pivotal role in organizing cellular structure, regulation of microtubule dynamics during adipogenesis remains unclear. In present paper we show that acetylation α-tubulin up-regulated adipogenesis, and adipocyte development dependent on acetylation, as expression an acetylation-resistant mutant significantly inhibits adipogenesis. Moreover,...