A5014309124- Hematopoietic Stem Cell Transplantation
- MicroRNA in disease regulation
- Cancer Genomics and Diagnostics
- Single-cell and spatial transcriptomics
- T-cell and B-cell Immunology
- Epigenetics and DNA Methylation
- Hedgehog Signaling Pathway Studies
- RNA Interference and Gene Delivery
- Mesenchymal stem cell research
- Acute Myeloid Leukemia Research
- Cancer Cells and Metastasis
- Extracellular vesicles in disease
- Histone Deacetylase Inhibitors Research
- Immune Cell Function and Interaction
- Myeloproliferative Neoplasms: Diagnosis and Treatment
- Genetics and Neurodevelopmental Disorders
- Genomics and Chromatin Dynamics
- HIV/AIDS Impact and Responses
- Advanced biosensing and bioanalysis techniques
- Immunotoxicology and immune responses
- Liver Disease Diagnosis and Treatment
- Genetic and Kidney Cyst Diseases
- Immunotherapy and Immune Responses
- HIV Research and Treatment
- Cancer-related gene regulation
University of Groningen
2008-2022
University Medical Center Groningen
2010-2022
Dialyse Centrum Groningen
2016
Hematopoietic stem cell (HSC) populations change with aging, but the extent to which this is caused by qualitative versus quantitative alterations in HSC subtypes unclear. Using clonal assays, study we show that aging compartment undergoes both and changes. We observed a variable increase of pool size age, accompanied accumulation predominantly myeloid-biased HSCs regenerate substantially fewer mature progeny than young exhibit reduced self-renewal activity as measured long-term secondary...
In a forward genetic screen in Drosophila melanogaster , aimed to identify genes required for normal locomotor function, we isolated dPPCS (the second enzyme of the Coenzyme A biosynthesis pathway). The entire CoA synthesis route was dissected, annotated and additional mutants were obtained ( dPANK/fumble ) or generated dPPAT-DPCK ). suffer from neurodegeneration, altered lipid homeostasis larval brains display increased apoptosis. Also, de novo is maintain DNA integrity during development...
Hematopoietic stem cells (HSCs) are able to migrate through the blood stream and engraft bone marrow (BM) niches. These features key factors for successful cell transplantations that used in cancer patients gene therapy protocols. It is unknown what extent transplanted HSCs distribute throughout different anatomical niches BM whether this changes with age. Here we determine degree of hematopoietic migration at a clonal level by transplanting individual young aged mouse labeled barcoded viral...
Accurate monitoring of tumor dynamics and leukemic stem cell (LSC) heterogeneity is important for the development personalized cancer therapies. In this study, we experimentally induced distinct types leukemia in mice by enforced expression Cbx7. Simultaneous cellular barcoding allowed thorough analysis leukemias at clonal level revealed high unpredictable complexity. Multiple LSC clones with properties coexisted. Some these remained dormant but bore potential, as they progressed to...
Expansion of hematopoietic stem cells (HSCs) is a 'holy grail' regenerative medicine, as successful cell transplantations depend on the number and quality infused HSCs. Although many attempts have been pursued to either chemically or genetically increase HSC numbers, neither clonal analysis these expanded nor their ability support mature blood lineages has demonstrated. Here we show that miR-125a, at single level, can expand murine long-term repopulating In addition, miR-125a increases clone...
Sonic hedgehog (SHH) medulloblastoma originates from the cerebellar granule neuron progenitor (CGNP) lineage, which depends on Hedgehog signaling for its perinatal expansion. Whereas SHH tumors exhibit overall deregulation of this pathway, they also show patient age-specific aberrations. To investigate whether developmental stage CGNP can account these lesions, we analyzed developing murine transcriptomes and observed highly dynamic gene expression as a function age. Cross-species comparison...