A5088204377- Acute Lymphoblastic Leukemia research
- Cancer Genomics and Diagnostics
- MicroRNA in disease regulation
- Epigenetics and DNA Methylation
- Single-cell and spatial transcriptomics
- Hematopoietic Stem Cell Transplantation
- Acute Myeloid Leukemia Research
- RNA Interference and Gene Delivery
- Genomics and Chromatin Dynamics
- Mesenchymal stem cell research
- Cancer-related Molecular Pathways
- Cancer Cells and Metastasis
- Cancer, Hypoxia, and Metabolism
- Microtubule and mitosis dynamics
- Myeloproliferative Neoplasms: Diagnosis and Treatment
- RNA modifications and cancer
- Childhood Cancer Survivors' Quality of Life
- Chronic Myeloid Leukemia Treatments
- Pancreatic function and diabetes
- T-cell and B-cell Immunology
- Colorectal Cancer Surgical Treatments
- Cell Image Analysis Techniques
- DNA Repair Mechanisms
- Glioma Diagnosis and Treatment
- Carcinogens and Genotoxicity Assessment
University of Groningen
2006-2025
University Medical Center Groningen
2006-2025
Genomics (United Kingdom)
2023
Dialyse Centrum Groningen
2016
Bioinformatics Institute
2012
University of Amsterdam
2010
Erasmus University Rotterdam
2008-2009
Erasmus MC - Sophia Children’s Hospital
2006-2009
Stanford University
2008
Rotterdam University of Applied Sciences
2008
While comprehensive molecular profiling of histone H3.3 mutant pediatric high-grade glioma has revealed extensive dysregulation the chromatin landscape, exact mechanisms driving tumor formation remain poorly understood. Since gliomas also exhibit high levels copy number alterations, we set out to address if H3.3K27M oncohistone leads destabilization genome. Hereto, established a cell culture model allowing inducible expression and observed an increase in mitotic abnormalities. We found...
Hematopoietic stem cells (HSCs) are able to migrate through the blood stream and engraft bone marrow (BM) niches. These features key factors for successful cell transplantations that used in cancer patients gene therapy protocols. It is unknown what extent transplanted HSCs distribute throughout different anatomical niches BM whether this changes with age. Here we determine degree of hematopoietic migration at a clonal level by transplanting individual young aged mouse labeled barcoded viral...
Abstract The transcription factor C/EBPβ is a master regulator of mammary gland development and tissue remodelling during lactation. CEBPB -mRNA translated into three distinct protein isoforms named C/EBPβ-LAP1, -LAP2 -LIP that are functionally different. smaller isoform LIP lacks the N-terminal transactivation domains considered to act as an inhibitor transactivating LAP1/2 by competitive binding for same DNA recognition sequences. Aberrantly high expression associated with epithelial...
ABSTRACT Medium chain acyl‐CoA dehydrogenase deficiency (MCADD) is an inherited metabolic disease, characterized by biallelic variants in the ACADM gene. Interestingly, even with same genotype, patients often present very heterogeneous symptoms, ranging from fully asymptomatic to life‐threatening hypoketotic hypoglycemia. The mechanisms underlying this heterogeneity remain unclear. Therefore, there a need for vitro models of MCADD that recapitulate clinical phenotype as tool study...
Accurate monitoring of tumor dynamics and leukemic stem cell (LSC) heterogeneity is important for the development personalized cancer therapies. In this study, we experimentally induced distinct types leukemia in mice by enforced expression Cbx7. Simultaneous cellular barcoding allowed thorough analysis leukemias at clonal level revealed high unpredictable complexity. Multiple LSC clones with properties coexisted. Some these remained dormant but bore potential, as they progressed to...
Cancer cells use glycolysis for generation of metabolic intermediates and ATP needed cell growth proliferation. The transcription factor C/EBPβ-LIP stimulates mitochondrial respiration in cancer cells. We initially observed that high expression makes vulnerable to treatment with the inhibitor 2-deoxyglucose. aim study was uncover involved mechanisms induced sensitivity inhibition.We used genetically engineered lines examine effect -LAP protein isoforms on NADH/NAD+ metabolism mouse embryonic...
Expansion of hematopoietic stem cells (HSCs) is a 'holy grail' regenerative medicine, as successful cell transplantations depend on the number and quality infused HSCs. Although many attempts have been pursued to either chemically or genetically increase HSC numbers, neither clonal analysis these expanded nor their ability support mature blood lineages has demonstrated. Here we show that miR-125a, at single level, can expand murine long-term repopulating In addition, miR-125a increases clone...