A5020161554- Antimicrobial Peptides and Activities
- Immune Response and Inflammation
- Neonatal Respiratory Health Research
- Inhalation and Respiratory Drug Delivery
- Pediatric health and respiratory diseases
- Chronic Obstructive Pulmonary Disease (COPD) Research
- Bacterial biofilms and quorum sensing
- Probiotics and Fermented Foods
- Pneumonia and Respiratory Infections
- Asthma and respiratory diseases
- Biochemical and Structural Characterization
- Respiratory viral infections research
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Cystic Fibrosis Research Advances
- Machine Learning in Bioinformatics
- Antibiotic Resistance in Bacteria
- Immune cells in cancer
- NF-κB Signaling Pathways
- Advanced biosensing and bioanalysis techniques
- Lipid Membrane Structure and Behavior
- RNA and protein synthesis mechanisms
- Heat shock proteins research
- Antimicrobial agents and applications
- RNA modifications and cancer
- Cancer-related gene regulation
University of Pittsburgh
2016-2025
Helmholtz Zentrum München
2025
Technical University of Munich
2025
Ministry of Industry and Information Technology
2025
Northwestern Polytechnical University
2025
Sichuan Agricultural University
2025
Northeast Agricultural University
2025
Chengdu University of Technology
2025
Chengdu University of Information Technology
2024
Rush University Medical Center
2022
Abstract Mesenchymal stem cells (MSCs) and macrophages are fundamental components of the cell niche function coordinately to regulate haematopoietic self-renewal mobilization. Recent studies indicate that mitophagy healthy mitochondrial critical survival cells, but how these processes regulated in MSCs is unknown. Here we show manage intracellular oxidative stress by targeting depolarized mitochondria plasma membrane via arrestin domain-containing protein 1-mediated microvesicles. The...
To better understand the molecular basis of chronic obstructive pulmonary disease (COPD), we used serial analysis gene expression (SAGE) and microarray to compare patterns lung tissues from COPD control smokers. A total 59,343 tags corresponding 26,502 transcripts were sequenced in SAGE analyses. 327 genes differentially expressed (1.5-fold up- or down-regulated). Microarray using same RNA source detected 261 that a significant degree between GOLD-2 GOLD-0 We confirmed altered select number...
Suppression of type 17 immunity by I interferon (IFN) during influenza A infection has been shown to enhance susceptibility secondary bacterial pneumonia. Although this mechanism described in coinfection with gram-positive bacteria, it is unclear whether similar mechanisms may impair lung defense against gram-negative infections. Furthermore, precise delineation the duration IFN-associated remains underexplored. Therefore, we investigated effects preceding virus on subsequent challenge...
Reactive oxygen species (ROS) can induce premature cellular senescence, which is believed to contribute aging and age-related diseases. The nuclear erythroid 2 p45–related factor-2 (Nrf2) a transcription factor that mediates cytoprotective responses against stress. We demonstrate caveolin-1 direct binding partner of Nrf2, as shown by the scaffolding domain (amino acids 82–101) caveolin-binding Nrf2 281–289). Biochemical studies show concentrated into caveolar membranes in human mouse...
Enantiomeric optimization markedly increased safety and efficacy of a cationic peptide against multidrug-resistant pathogens.
Due to their excellent in vitro activity against multidrug resistant bacteria, antimicrobial peptides (AMPs) hold promise for treatment of Pseudomonas aeruginosa lung infections cystic fibrosis (CF) sufferers. In this work, poly(lactide- co-glycolide) (PLGA) nanoparticles delivery AMPs deriving from the frog-skin esculentin-1a, namely, Esc(1-21) and Esc(1-21)-1c (Esc peptides), were successfully developed. Improved peptide transport through artificial CF mucus simulated bacterial...
Chronic obstructive pulmonary disease (COPD) is an inflammatory that associated with increased risk of lung cancer.
Anoxia, glucose starvation, calcium ionophore A23187, EDTA, glucosamine, and several other conditions that adversely affect the function of endoplasmic reticulum (ER) induce synthesis glucose-regulated class stress proteins (GRPs). The primary GRPs induced by these stresses migrate at 78 94 kDa (GRP78 GRP94). In addition, another protein approximately 150-170 (GRP170) has been previously observed is coordinately with GRP78 GRP94. To characterize this novel protein, we have prepared an...
Abstract Maintenance of classic stem cell hierarchies is dependent upon self-renewal mediated in part by Wnt/β-catenin regulation the cycle. This function critical rapidly renewing tissues due to obligate role played tissue cell. However, hierarchy responsible for maintenance conducting airway epithelium distinct from hierarchies. The airways maintained transit-amplifying cells steady state; rare bronchiolar are activated participate epithelial repair only following depletion cells. Here, we...
Epithelial host defense proteins comprise a critical component of the pulmonary innate immune response to infection. The short palate, lung, nasal epithelium clone (PLUNC) 1 (SPLUNC1) protein is member bactericidal/permeability-increasing (BPI) fold-containing (BPIF) family, sharing structural similarities with BPI-like proteins. SPLUNC1 25 kDa secretory that expressed in nasal, oropharyngeal, and lung epithelia, has been implicated airway against Pseudomonas aeruginosa other organisms....
Human mucin (MUC) 5B gene expression in human airway epithelium was studied both tissue sections and cultures of tracheobronchial epithelial (TBE) cells. In situ hybridization demonstrated that MUC5B message expressed mainly the mucous cells submucosal glands normal tissues. Nevertheless, an elevated level could be seen surface goblet from patients with diseases inflammation. Regardless sources, regulated by all-trans-retinoic acid (RA) culture conditions primary passage-1 TBE message, to a...
Epithelial antimicrobial activity may protect the lung against inhaled pathogens. The bactericidal/permeability-increasing protein family has demonstrated in vitro. PLUNC (palate, lung, and nasal epithelium associated) is a 25-kDa secreted that shares homology with proteins expressed nasopharyngeal respiratory epithelium. objective of this study was to determine whether can limit Pseudomonas aeruginosa infection mice. Transgenic mice (Scgb1a1-hPLUNC) were generated which human (hPLUNC)...
Abstract The airway epithelium is the first line of host defense against pathogens. short palate, lung, and nasal clone (SPLUNC)1 protein secreted in respiratory tracts a member bacterial/permeability increasing (BPI) fold–containing family, which shares structural similarities with BPI-like proteins. On basis its homology BPIs restricted expression SPLUNC1 serous cells submucosal glands surface epithelial upper tract, thought to possess antimicrobial activity defense. also reported have...
Pseudomonas aeruginosa is the major microorganism colonizing respiratory epithelium in cystic fibrosis (CF) sufferers. The widespread use of available antibiotics has drastically reduced their efficacy, and antimicrobial peptides (AMPs) are a promising alternative. Among them, frog skin-derived AMPs, i.e., Esc(1-21) its diastereomer, Esc(1-21)-1c, have recently shown potent activity against free-living sessile forms P. Importantly, this pathogen also escapes treatment by invading airway...
Pseudomonas aeruginosa is a pathogenic bacterium known to cause serious human infections, especially in immune‐compromised patients. This due its unique ability transform from drug‐tolerant planktonic more dangerous and treatment‐resistant sessile life form, called biofilm. Recently, two derivatives of the frog skin antimicrobial peptide esculentin‐1a, i.e. Esc(1‐21) D‐amino acids containing diastereomer Esc(1‐21)‐1c, were characterized for their powerful anti‐ Pseudomonal activity against...
Club (Clara) Cell Secretory Protein (CCSP, or CC16) is produced mainly by non-ciliated airway epithelial cells including bronchiolar club and the change of its expression has been shown to associate with progress severity Chronic Obstructive Pulmonary Disease (COPD). In an animal model, lack CC16 renders susceptible tumorigenic effect a major CS carcinogen. A recent population-based Tucson Epidemiological Study Airway Diseases (TESAOD) indicated that low serum concentration closely linked...