A5025546511- Skin and Cellular Biology Research
- Hair Growth and Disorders
- Blood properties and coagulation
- Skin Protection and Aging
- Wound Healing and Treatments
- Retinoids in leukemia and cellular processes
- Molecular Biology Techniques and Applications
- Carcinogens and Genotoxicity Assessment
- Tendon Structure and Treatment
- Plant Reproductive Biology
- Arsenic contamination and mitigation
- Genomics, phytochemicals, and oxidative stress
- Plant Surface Properties and Treatments
- melanin and skin pigmentation
- Effects and risks of endocrine disrupting chemicals
- Toxic Organic Pollutants Impact
- Wnt/β-catenin signaling in development and cancer
- Advanced Proteomics Techniques and Applications
- Cellular Mechanics and Interactions
- RNA regulation and disease
- Contact Dermatitis and Allergies
- Nail Diseases and Treatments
- Dermatology and Skin Diseases
- Advancements in Transdermal Drug Delivery
- Pharmacogenetics and Drug Metabolism
University of California, Davis
2015-2024
Augusta University
2023
Augusta University Health
2023
Broad Institute
2016-2019
Society of Environmental Toxicology and Chemistry
1994-2016
Digital Proteomics (United States)
2016
University of Colorado Anschutz Medical Campus
2016
University of Veterinary Medicine Hannover, Foundation
2014
University of California, Berkeley
1971-2005
University of California, San Francisco
2002-2003
A procedure is described for the radioactive labeling of proteins in vitro which 3H-or 14C-methyl groups are attached to protein amino by reductive alkylation.Several and several viruses have been labeled specific activities typically 5 X lo6 cpm per mg protein.Some applications illustrated.
When serially cultivated human epidermal keratinocytes are placed in suspension culture they stop growing and form, beneath the plasma membrane, an insoluble envelope consisting of protein cross-linked by ε- (γ-glutamyl)lysine. The formation envelopes suspended cells is preceded a sharp decline rate synthesis, most appear only after average synthesis has fallen to very low level. If reduced over 98 percent with cycloheximide or emetine at time that surface-grown culture, form cells. This...
Human identification from biological material is largely dependent on the ability to characterize genetic polymorphisms in DNA. Unfortunately, DNA can degrade environment, sometimes below level at which it be amplified by PCR. Protein however chemically more robust than and persist for longer periods. also contains variation form of single amino acid polymorphisms. These used infer status non-synonymous nucleotide polymorphism alleles. To demonstrate this, we mass spectrometry-based shotgun...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTChemical modification and hybridization of wheat germ agglutininsRobert H. Rice Marilynn E. EtzlerCite this: Biochemistry 1975, 14, 18, 4093–4099Publication Date (Print):September 1, 1975Publication History Published online1 May 2002Published inissue 1 September 1975https://pubs.acs.org/doi/10.1021/bi00689a027https://doi.org/10.1021/bi00689a027research-articleACS PublicationsRequest reuse permissionsArticle Views175Altmetric-Citations107LEARN ABOUT...
The evolution of amniotes has involved major molecular innovations in the epidermis. In particular, distinct structural proteins that undergo covalent cross-linking during cornification keratinocytes facilitate formation mechanically resilient superficial cell layers and help to limit water loss environment. Special modes generate amniote-specific skin appendages such as claws, feathers, hair. mammals, many protein substrates are encoded by a cluster genes, termed epidermal differentiation...
Keratin intermediate filaments (KIFs) protect the epidermis against mechanical force, support strong adhesion, help barrier formation, and regulate growth. The mechanisms by which type I II keratins contribute to these functions remain incompletely understood. Here, we report that mice lacking all or display severe defects fragile skin, leading perinatal mortality with full penetrance. Comparative proteomics of cornified envelopes (CEs) from prenatal KtyI−/− KtyII−/−K8 demonstrates absence...
The human hair proteome was investigated using two-dimensional LC-MS/MS. Among the 343 identified proteins, 70 were detected in high relative abundance, including keratin intermediate filament largely extractable with denaturants. Over 300 proteins found to constitute insoluble complex formed by transglutaminase cross-linking. intracellular distribution of is wide from cytoplasm nucleus, mitochondria, ribosome, and plasma membrane. These results help rationalize ultrastructural features...
The nucleotide and deduced amino acid sequences of the coding regions human rat keratinocyte transglutaminases (protein-glutamine: amine gamma-glutamyltransferase; EC 2.3.2.13) have been determined. These yield proteins approximately 90 kDa that are 92% identical, indicative conservation important structural features. Alignments show substantial similarity among transglutaminase, clotting factor XIII catalytic subunit, guinea pig liver tissue erythrocyte band-4.2 protein. enzyme is most...
Hair from different individuals can be distinguished by physical properties. Although some data exist on other species, examination of the individual molecular differences within human hair shaft has not been thoroughly investigated. Shotgun proteomic analysis revealed considerable variation in profile among samples Caucasian, African–American, Kenyan and Korean subjects. Within these ethnic groups, prominent keratin proteins served to distinguish profiles. Differences between less marked,...
Abstract: The basis for the permeability barrier abnormality in lamellar ichthyosis (LI) is not known. LI caused by mutations gene that encodes enzyme, transglutaminase 1 (TGI), which responsible assembly of cornified envelope (CE). TG1 also has been suggested recently to catalyze covalent attachment omega‐hydroxyceramides (omega‐OHCer) CE, forming corneocyte‐lipid (CLE). We first assessed function and pathway water‐soluble tracer, colloidal lanthanum, across stratum corneum (SC) patients...
The membrane-bound form of keratinocyte transglutaminase was found to be labeled by addition [3H] acetic, [3H]myristic, or [3H]palmitic acids the culture medium human epidermal cells. Acid methanolysis and high performance liquid chromatography analysis palmitate-labeled yielded only methyl palmitate. In contrast, myristate-labeled protein approximately 40% myristate 60% Incorporation neither label significantly affected cycloheximide inhibition synthesis. importance fatty acid moiety for...
Alteration of gene expression by inorganic arsenic has been studied in cultured human keratinocytes derived from normal epidermis, a premalignant lesion and malignant tumor. The purpose was to find whether these cells displayed common alterations that might elucidate the mechanism action. Global analysis ∼12 000 genes microarray showed ∼30% were expressed. Of these, transcription substantial fraction (up 12%) altered, nearly twice as many being suppressed stimulated 2-fold or more at 2 µM...