A5084689376- Plant tissue culture and regeneration
- Chromosomal and Genetic Variations
- Plant Disease Resistance and Genetics
- Plant Reproductive Biology
- Photosynthetic Processes and Mechanisms
- Agricultural Practices and Plant Genetics
- Garlic and Onion Studies
- Agriculture, Plant Science, Crop Management
- Seed and Plant Biochemistry
- Antioxidant Activity and Oxidative Stress
- Spectroscopy and Chemometric Analyses
- Plant Molecular Biology Research
- Plant Virus Research Studies
- Potato Plant Research
- Growth and nutrition in plants
- Plant Pathogens and Fungal Diseases
- Plant chemical constituents analysis
- Flowering Plant Growth and Cultivation
- Transgenic Plants and Applications
- Magnetic and Electromagnetic Effects
- Plant Pathogens and Resistance
- Advances in Cucurbitaceae Research
- Genetic diversity and population structure
- Plant biochemistry and biosynthesis
- Plant Genetic and Mutation Studies
University of Agriculture in Krakow
2016-2025
Institute of Agricultural and Food Biotechnology
2019
University of Agriculture Faisalabad
2011-2014
Academy of Music in Kraków
2003
Philipp Simon, Massimo Iorizzo, Allen Van Deynze and colleagues report the high-quality assembly of carrot genome, providing an important resource for crop improvement. They find a candidate gene that regulates carotenoid accumulation gain further insights into asterid genome evolution, including characterization two new polyploidization events. We chromosome-scale analysis (Daucus carota) first sequenced to include comparative evolutionary among members euasterid II clade. characterized...
An easy and effective regeneration system from leaf- hypocotyl-derived protoplasts was established for carrot. The protoplast isolation efficiency after preplasmolytic treatment digestion of source material in enzyme mixture consisted 1% cellulase Onozuka R-10 0.1% pectolyase Y-23 reached on average 3 × 106 per g leaf hypocotyl tissue, respectively. A modified thin alginate layer technique applied the culture. Direct somatic embryogenesis a simplified Kao Michayluk medium presence 2,4-D...
Abstract In this study we report the development of effective in vitro systems for a medicinal plant Nigella damascena L. comprising: (1) callus induction, (2) somatic embryogenesis cultures with subsequent regeneration, and (3) isolation regeneration callus-derived protoplasts. Callus was achieved on 83–100% hypocotyl cotyledon explants, whereby Murashige Skoog medium (MS) supplemented 3 mg L −1 6-benzylaminopurine 0.5 α -naphthaleneacetic acid (NAA; BN medium) more advantageous than MS...
Protoplasts of four Daucus carota subspecies and three wild species were isolated from 2-week-old shoot cultures during overnight incubation in an enzyme mixture composed 1 % (w/v) cellulase Onozuka R-10 0.1 pectolyase Y-23. Before the culture, they embedded autoclave- or filter-sterilized alginate solution. Modified thin layer (TAL) extra film (ETAF) techniques applied for protoplast immobilization. A rich mineral-organic medium based on formulation Kao Michayluk supplemented with mg l−1...
Carrot is one of the most important vegetables worldwide, owing to its capability develop fleshy, highly nutritious storage roots. It was domesticated ca. 1,100 years ago in Central Asia. No systematic knowledge about molecular mechanisms involved domestication syndrome carrot are available, however, ability form a root undoubtedly essential transition from wild Daucus carota cultivated carrot. Here, we expand on results previous study which identified polymorphism showing significant...
This is the first study to generate carrot plants for enhanced salinity tolerance using a single-cell in vitro system. Protoplasts of three accessions were exposed treatment by seven different concentrations NaCl (10–400 mM). Salt higher than 50 mM decreased plating efficiency and those 200–400 completely arrested mitotic divisions cultured cells. The protoplast-derived from control 50–100 subjected an 8-week salt stress greenhouse conditions induced salinized soil (EC 3 6 mS cm−1). applied...
Abstract Carotenoid microcrystals, extracted from cells of carrot roots and consisting 95 % achiral β‐carotene, exhibit a very intense chiroptical (ECD ROA) signal. The preferential chirality crystalline aggregates that consist mostly building blocks is newly observed phenomenon in nature, may be related to asymmetric information transfer the chiral seeds (small amount α‐carotene or lutein) present cells. To confirm this hypothesis, we synthesized several model various carotenoids. Because...
Karyotype analysis provides insights into genome organization at the chromosome level and evolution. Chromosomes were marked for comparative karyotype using FISH localization of rDNA genes first time in Apioideae species including taxa economic importance several wild Daucus relatives. Interestingly, did not vary number loci despite variation (2n = 18, 20, 22, 44) previous publications suggesting multiple loci. All had single both 5S 18S-25S (nucleolar organizing region) rDNA, located on two...
Protoplasts of three carrot cultivars were isolated from in vitro-grown plantlets by overnight incubation an enzyme mixture composed 1% (w/v) cellulase Onozuka R-10 and 0.1% pectolyase Y-23. After cell immobilization modified thin alginate layers, types β-lactam antibiotics (cefotaxime, carbenicillin, or timentin) at five different concentrations (100, 200, 300, 400, 500 mg L-1) added to the culture medium. In 20-d-old cultures, a number colonies had formed varied on average 27 56%...
Brassica oleracea L. is a key plant in the Brassicaceae family, known for popular vegetables like cabbage, broccoli, kale and collard. Collard (B. var. viridis) non-heading leafy vegetable grown urban farms community gardens United States Europe. Improving collard other germplasm can benefit from both traditional new breeding technologies (NPBTs), such as CRISPR-Cas mediated transformation. An efficient transformation or protoplast fusion only be achieved with robust reproducible protocol...
The aim of this work was to improve plating efficiency sugar beet mesophyll protoplast cultures. Preliminary experiments showed that cultures good quality, viable protoplasts were obtained in rich media based on the Kao and Michayluk formulation with calcium alginate as an embedding matrix. Nevertheless, these cell divisions either not observed or very seldom confirming earlier reported recalcitrance protoplasts. recalcitrant status reversed upon application exogenous phytosulfokine (PSK)—a...
Abstract In the present study, a high yield of isolated protoplasts from agronomically important crop Fagopyrum esculentum was obtained by applying mixture cellulase, pectolyase, and driselase. We demonstrated that morphogenic callus-derived 1 × 10 6 per g fresh tissue. For hypocotyls used as protoplast source, number released cells twice lower. The protoplasts, embedded in an agarose matrix cultured modified Kao Michayluk media supplemented with phytosulfokine, re-enter cell cycle start to...
Fagopyrum tataricum (Tartary buckwheat) is a valuable crop of great nutritional importance due to its high level bioactive compounds. Excellent opportunities obtain plants with the or desired profile metabolites may be provided by in vitro cultures. Among known techniques, protoplast technology an exciting tool for genetic manipulation improve traits. In that context, fusion applied generate hybrid cells between different species Fagopyrum. To apply cultures aforementioned approaches this...
Phytosulfokine-α (PSK), a peptidyl plant growth factor, has been recognized as promising intercellular signaling molecule involved in cellular proliferation and dedifferentiation. It was shown that PSK stimulated enhanced cell divisions protoplast cultures of several species leading to callus proembryogenic mass formation. Since had cause an increase efficiency somatic embryogenesis, it reasonable check the distribution selected chemical components walls during regeneration process. So far,...
Abstract The cell wall rebuilding is one of the first stage protoplast development that enables further mitotic divisions and differentiation. Therefore, this work focuses on comparison regeneration in parental protoplasts Fagopyrum tataricum , F. esculentum (+) hybrids, which are promising materials terms future breeding research programmes. It worth emphasizing preparation buckwheat hybrids using electrofusion was described for time. results indicate exhibited a common mechanism parent...
We investigated nine families of Stowaway-like miniature inverted-repeat transposable elements (MITEs) in the carrot genome, named DcSto1 to DcSto9. All them were AT-rich and shared a highly conserved 6 bp-long TIR typical for Stowaways. The copy number was estimated as ca. 5,000 per diploid genome. observed preference clustered insertions DcSto other MITEs. Distribution hybridization signals revealed presence clusters within euchromatic regions along all chromosomes. An arrangement eight...