A5037807780- Genomics and Chromatin Dynamics
- CRISPR and Genetic Engineering
- Chromatin Remodeling and Cancer
- Epigenetics and DNA Methylation
- RNA Research and Splicing
- RNA and protein synthesis mechanisms
- DNA and Nucleic Acid Chemistry
- Data Visualization and Analytics
- Pluripotent Stem Cells Research
- Histone Deacetylase Inhibitors Research
- Advanced biosensing and bioanalysis techniques
- RNA modifications and cancer
- Chromosomal and Genetic Variations
- RNA regulation and disease
- Academic Writing and Publishing
Lewis & Clark College
2018-2024
Howard Hughes Medical Institute
2016-2017
University of California, Berkeley
2016
Universidad Católica Santo Domingo
2015
University of California, San Diego
2011-2015
Pitzer College
2008
The prokaryotic CRISPR (clustered regularly interspaced palindromic repeats)-associated protein, Cas9, has been widely adopted as a tool for editing, imaging, and regulating eukaryotic genomes. However, our understanding of how to select single-guide RNAs (sgRNAs) that mediate efficient Cas9 activity is incomplete, we lack insight into chromatin impacts targeting. To address this gap, analyzed large-scale genetic screens performed in human cell lines using either nuclease-active or...
Chromatin comprises nucleosomes as well nonnucleosomal histone–DNA particles. Prenucleosomes are rapidly formed particles that can be converted into canonical by a motor protein such ACF. Here we show the prenucleosome is stable conformational isomer of nucleosome. It consists histone octamer associated with ∼80 base pair (bp) DNA, which located at position corresponds to central 80 bp nucleosome core particle. Monomeric prenucleosomes free flanking DNA do not spontaneously fold but an...
Transcription factor (TF)-directed enhanceosome assembly constitutes a fundamental regulatory mechanism driving spatiotemporal gene expression programs during animal development. Despite decades of study, we know little about the dynamics or order events animating TF at cis-regulatory elements in living cells and long-range molecular "dialog" between enhancers promoters. Here, combining genetic, genomic, imaging approaches, characterize complex enhancer cluster governing Krüppel-like 4...
Chromatin assembly involves the combined action of ATP-dependent motor proteins and histone chaperones. Because in chromatin also function as remodeling factors, we investigated relationship between ATP-driven generation periodic nucleosome arrays. We found that remodeling-defective Chd1 are able to catalyze assembly. The resulting nucleosomes not, however, spaced Wild-type Chd1, but not can conversion randomly-distributed into These results reveal a functional distinction remodeling,...
ABSTRACT Newly synthesized histones are acetylated prior to their deposition into nucleosomes. Following nucleosome formation and positioning, they rapidly deacetylated, an event that coincides with further maturation of the chromatin fiber. The histone deacetylases (HDACs) used for de novo poorly understood. In ciliate Tetrahymena thermophila , transcription-related deacetylation in macronucleus is physically separated from deposition-related micronucleus. This feature was utilized identify...
Enhancers have critical functions in the precise, spatiotemporal control of transcription during development. It is thought that enhancer grammar, or characteristics and arrangements factor binding sites, underlie specific developmental enhancers. In this study, we sought to identify grammatical constraints direct activity naïve state pluripotency, focusing on enhancers for naïve-state gene, Klf4 . Using a combination biochemical tests, reporter assays, endogenous mutations mouse embryonic...
Abstract A poster is one method of informing the world about results from a research project. Whether serving as an aid for presentation or standing alone, it should provide information Both substantive and stylistic qualities contribute to effectively communicating one's conclusions. This article gives general guidelines suggestions content, layout, design assist in preparing effective poster. © 2018 by John Wiley & Sons, Inc.