A5054309276- Probiotics and Fermented Foods
- Microbial Metabolites in Food Biotechnology
- Gut microbiota and health
- Enzyme Production and Characterization
- Food composition and properties
- Infant Nutrition and Health
- Protein Hydrolysis and Bioactive Peptides
- Bacteriophages and microbial interactions
- Biofuel production and bioconversion
- Enzyme Structure and Function
- Microbial Metabolic Engineering and Bioproduction
- Glycosylation and Glycoproteins Research
- Advanced Proteomics Techniques and Applications
- Digestive system and related health
- Carbohydrate Chemistry and Synthesis
- Genomics and Phylogenetic Studies
- Microbial Metabolism and Applications
- Bacterial Genetics and Biotechnology
- Drug Transport and Resistance Mechanisms
- RNA and protein synthesis mechanisms
- RNA Research and Splicing
- RNA modifications and cancer
- Pediatric Hepatobiliary Diseases and Treatments
- Cancer Research and Treatments
- Metabolism and Genetic Disorders
Technical University of Denmark
2011-2019
Digital Proteomics (United States)
2016
University of Copenhagen
2007
Abstract β-Mannans are plant cell wall polysaccharides that commonly found in human diets. However, a mechanistic understanding into the key populations degrade this glycan is absent, especially for dominant Firmicutes phylum. Here, we show prominent butyrate-producing Firmicute Roseburia intestinalis expresses two loci conferring metabolism of β-mannans. We combine multi-“omic” analyses and detailed biochemical studies to comprehensively characterize loci-encoded proteins involved β-mannan...
The human gut microbiota established during infancy has persistent effects on health. In vitro studies have suggested that milk oligosaccharides (HMOs) in breast promote the formation of a bifidobacteria-rich infant guts; however, underlying molecular mechanism remains elusive. Here, we characterized two functionally distinct but overlapping fucosyllactose transporters (FL transporter-1 and -2) from Bifidobacterium longum subspecies infantis. Fecal DNA HMO consumption analyses, combined with...
Summary Glycan utilization plays a key role in modulating the composition of gut microbiota, but molecular insight into oligosaccharide uptake by this microbial community is lacking. Arabinoxylo‐oligosaccharides ( AXOS ) are abundant diet, and selectively fermented probiotic bifidobacteria colon. Here we show how selectivity for established strain B ifidobacterium animalis subsp. lactis Bl ‐04. The binding protein AXBP , which associated with an ATP ‐binding cassette ABC transporter that...
Lactobacillus acidophilus NCFM is a probiotic bacterium adapted to survive in the gastrointestinal tract and with potential health benefits host. Lactitol synthetic sugar alcohol used as replacement low calorie foods selectively stimulating growth of L. NCFM. In present study whole-cell extract proteome grown on glucose until late exponential phase was resolved by 2-DE (pH 3-7). A total 275 unique proteins assigned various physiological processes were identified from 650 spots. Differential...
Escherichia coli strains with inactivated rluD genes were previously found to lack the conserved pseudouridines in helix 69 of 23S ribosomal RNA and grow slowly. A suppressor mutant was isolated a near normal growth rate that had changed Glu-172 codon Lys prfB, encoding translation termination factor RF2. When nonsense suppression all combinations prfB(+)/prfB(E172K) rluD(+)/rluD::cat analyzed, misreading three stop codons as sense be increased by inactivation: Nonsense 2-fold at UAG codons,...
Whole cell and surface proteomes were analyzed together with adhesive properties of the probiotic bacterium Lactobacillus acidophilus NCFM (NCFM) grown on emerging prebiotic raffinose, exemplifying a synbiotic. Adhesion to mucin intestinal HT‐29 cells increased three‐fold after culture raffinose versus glucose, as also visualized by scanning electron microscopy. Comparative proteomics using 2D‐DIGE showed 43 unique proteins change in relative abundance whole lysates from compared glucose....
Abstract Human milk oligosaccharides (HMOs) are uniquely rich in the type 1 building block disaccharide lacto- N -biose I (LNB, Galβ1,3GlcNAc), as compared to other mammals. Most HMOs fucosylated, e.g. , α1,2 and α1,4 fucosylations on LNB blocks, resulting H (H1) Lewis a (Le ) epitopes, respectively. The dominance of Bifidobacterium breastfed infant guts hinges efficient uptake by specific ATP-binding cassette (ABC) importers. However, molecular insight into fucosylated blocks is lacking....
Summary Human gut bifidobacteria rely on ATP‐binding cassette (ABC) transporters for oligosaccharide uptake. Multiple oligosaccharide‐specific solute‐binding protein (SBP) genes are occasionally associated with a single ABC transporter, but the significance of this multiplicity remains unclear. Here, we characterize Bl MnBP1 and MnBP2, two SBPs to β‐manno‐oligosaccharide (MnOS) transporter in Bifidobacterium animalis subsp. lactis . Despite similar overall specificity preference mannotriose...
In the present study we examine changes in expression of genes Lactococcus lactis subspecies cremoris MG1363 during growth milk. To reveal which specific classes (pathways, operons, regulons, COGs) are important, performed a transcriptome time series experiment. Global analysis gene over showed that L. adapted quickly to environmental changes. Using upstream sequences with correlated profiles, uncovered substantial number putative DNA binding motifs may be relevant for fermentative All...
In recent years, a plethora of studies have demonstrated the paramount physiological importance gut microbiota on various aspects human health and development. Particular focus has been set probiotic members this community, best studied which are assigned into Lactobacillus Bifidobacterium genera. Effects such as pathogen exclusion, alleviation inflammation allergies, colon cancer, other bowel disorders attributed to activity bacteria, selectively ferment prebiotics comprising mainly...
The organisation of genes conferring utilisation raffinose family oligosaccharides (RFOs) has been analysed in several probiotic bacteria from the Bifidobacterium and Lactobacillus genera. Glycoside hydrolase 36 (GH36) α-galatosidase encoding occur together with sugar transport systems glycoside–pentoside–hexuronide cation symporter (GPH), phosphotransferase (PTSs) or ATP-binding cassette (ABCs) highlighting diversity RFO uptake. GH36 are often clustered sucrose hydrolases phosphorylases...
Efficient capture of glycans, the prime metabolic resources in human gut, confers a key competitive advantage for gut microbiota members equipped with extracellular glycoside hydrolases (GHs) to target these substrates. The association glycans bacterial cell surface is typically mediated by carbohydrate binding modules (CBMs). Here, we report structure Ri CBM86 appended GH family 10 xylanase from Roseburia intestinalis . This CBM represents new xylan CBMs present xylanases abundant and...
L actobacillus acidophilus NCFM ( ) is a well‐documented probiotic bacterium isolated from human gut. Detailed 2D gel‐based proteomics addressed the so‐called alkaline range, i.e., pH 6–11. Proteins were identified in 150 of 202 spots picked C oomassie B rilliant lue stained gel using MALDI ‐ TOF MS . The 102 unique gene products among protein identifications assigned to different functional categories, and evaluated by considering calculated distribution abundance as well grand average...
DOI: 10.1002/pmic.201500212 Lactobacillus acidophilus NCFM is a commonly used and well-studied probiotic bacterium. By the present differential proteome analysis, new insights into its synbiotic combination with emerging prebiotic raffinose through comparative whole-cell surface proteomes coupled intestinal cell adhesion analysis are gained. This picture shows bacterial cells adhering to human colonic HT-29 as stimulated by (chemical formula) representative 2-DIGE image of proteins increased...