A methanolic crude extract of Parkia biglobosa was prepared and later partitioned in succession with different solvents increasing polarity ranging from n-hexane, chloroform ethyl acetate to butanol. Phytochemical screening the revealed presence alkaloids, tannins, saponins, flavonoids, steroids, glycoside sugars. The inhibition zones exhibited by against tested bacteria ranged between 14 ± 0.00 mm (against Escherichia coli) 28 0.71 Pseudomonas aeruginosa). MIC P. isolates 0.63 mg/mL 5...

The current study determines the density of Vibrio spp. and isolates V . cholerae mimicus from fish-anatomical-sites, prawn, crab mussel samples recovered fish markets, freshwater brackish water. Virulence antibiotic resistance profiling were carried out using standard molecular microbiology techniques. was detected in more than 90% [134/144] its significantly other samples. isolated at least one sample each type with higher isolation frequency All belong to non-O1/non-O139 serogroup. One or...

Water resources contaminated with pathogenic Vibrio species are usually a source of devastating infection outbreaks that have been public health concern in both developed and developing countries over the decades. The present study assessed prevalence six medically significant some water Eastern Cape Province, South Africa for 12 months. We detected vibrios all 194 samples analyzed using polymerase chain reaction (PCR). cholerae , mimicus fluvialis vulnificus alginolyticus parahaemolyticus...

Alchornea laxiflora leaf extract was tested against a range of microorganisms using standard microbiological methods for antimicrobial activities. The inhibited the growth all bacterial and 15 fungal isolates tested. zones inhibition exhibited test bacteria ranged between 12 mm 24 11 antibiotic streptomycin, respectively. observed by 23 mm. minimum inhibitory concentrations (MICs) bactericidal (MBCs) 0.78 mg/mL-25 mg/mL 1.56 mg/mL, respectively, while MICs fungicidal (MFCs) values fungi 8.75...

Virulence determinants are crucial to the risk assessment of pathogens in an environment. This study investigated presence eleven key virulence-associated genes Vibrio cholerae (n = 111) and mimicus 22) eight virulence alginolyticus 65) parahaemolyticus 17) isolated from six important water resources Eastern Cape, South Africa, using PCR techniques. The multiple gene indexes (MVGI) for sampling sites isolates as well hotspots potential vibriosis outbreaks among were determined statistically...

Treated sewage harbours pathogenic microbes, such as enteric bacteria and protozoa, are capable of causing several diseases. Some these emerging pathogens sometimes recovered in the absence common water quality indicator organisms. The possibility selected treatments plants serving momentary reservoirs Vibrio during a non-outbreak period was assessed. occurrence diversity were monitored for one year (December 2016 to November 2017) treated effluents upstream downstream areas receiving bodies...

The study investigated the antibacterial activity of essential oil from peel Citrus aurantifolia against eleven multidrug-resistant (MDR) bacterial isolates clinical origin. Kirby-Bauer disc diffusion method was used to determine antibiotic resistance profile isolates. Essential (EO) peels lime purchased at a market in Ile-Ife extracted by hydro-distillation method, while sensitivity EO done via agar well method. minimum inhibitory and bactericidal concentrations (MIC MBC) tested were...

Limpet (Scutellastra cochlear) serves as seafood recipe and an important member of the aquatic food chain. It is abundant mollusc in some environment South Africa. In this study, we investigated potential molluscs harvested from Buffalo, Swartkops Kowie estuaries Eastern Cape Province, Africa to serve transient or maintenance reservoir Vibrio species. The source water samples were collected monthly rivers between December 2016 November 2017. category limpet recovered was determined by...

Studies on the synthesis of some sulfa quinoxaline has generate a considerable reputation as result their outstanding biochemical properties. This recent study was designed to prepared hydrazone derivatives and studying antimicrobial potency. The 3-methyl-2-oxo-1,2-dihydroquinoxaline-6-sulfonohydrazone were synthesized by reactions 3-methyl-2-oxo-1,2-dihydroquinoxaline-6-sulfonohydrazide six substituted benzaldehydes examined for possible antibacterial synthetic compounds exhibited...

The incidence of resistance to commonly used antimicrobial agents by microbial pathogens demands increased effort in the development effective ways treating infections and diseases. n-hexane fraction lyophilized crude bark extract Parkia biglobosa (Jacq.) was prepared and, combination with selected antibiotics, assayed for activity against some bacterial using time-kill assay. Protein leakage analysis combined performed Bradford protein quantification method. Determination active compounds...

Both Gossypium barbadense (GB) and hirsitum (GH) are members of the Malvaceae family cotton genus. In order to reduce silver nitrate, aqueous extracts both plants were used synthesize biogenic nanoparticles (AgNPs) (AgNO3). The techniques employed describe them included X-ray diffraction, ultraviolet-visible spectroscopy, scanning electron microscopy, energy dispersive (EDX), transmission microscopy (TEM), fourier transformed infrared (FTIR) spectrophotometer. AgNPs found be crystalline...

Aim:This study aimed at exploring the hemagglutinating and antibacterial activities of crude lectin from fruit pulp Trilepisium madagascariense DC.Methods: Crude was obtained by maceration ammonium sulphate precipitation.Hemagglutinating activity assay carried out determining ability extract to agglutinate red blood cells, serial dilution done followed addition erythrocytes.The subjected evaluation for inhibition bacterial growth agar well diffusion method against fourteen human pathogenic...

Research studies have shown that mosses contain bioactive substances and are of significant economic importance. The aim this study was to analyze the composition Barbula lambarenensis screen for its bioactivity. moss collected from natural habitat air- dried at room temperature in laboratory, extracted with methanol crude extract utilized gas chromatography-mass spectrometry (GC-MS) analysis, antibacterial sensitivity testing generation time elongation assay. antimicrobial activity tested...