Plant-derived hydrolysates are emerging as promising agents in the management of diverse ailments due to their ensuing functional and bioactive properties. This study investigated functional, antioxidant, anti-inflammatory, antidiabetic properties from Erythrina senegalensis Vigna subtenarrea seeds. Crude proteins were isolated via alkaline solubilization, followed by acid precipitation isoelectric point. Respective protein isolates hydrolyzed using trypsin pepsin at an enzyme-substrate...

Background & Aims: Hydrolyzed food proteins contain bioactive peptides that can modulate physiological processes promote good health. In this study, isolated Tilapia zillii muscle protein (TzMP) and Oreochromis niloticus (OnMP) were enzymatically hydrolyzed, their antioxidant properties evaluated to determine therapeutic potential in the treatment of oxidative stress-related illness. Methods: The hydrolysates O. T. produced with digestive proteases: pepsin, trypsin, chymotrypsin, degree...

A lectin was isolated and purified from the pepper seeds Capsicum annuum. The purification procedure involved anionic exchange chromatography on DEAE ‐ Cellulose QAE Sephadex columns followed by gel filtration G‐100. heamagglutinating activity of towards human erythrocytes sensitive to inhibition D-mannose D-glucose; enhancement CaCl2 MgCl2. enhanced at very high acidic pH, inhibited basic pH but stable physiological range 6- 8. heat up 30°C. SDSPAGE revealed presence single band 25 kDa....

Allelopathy has gained much attention in recent times the sustainable agricultural systems. Allelochemicals released by different plants interact differently with each other, it therefore becomes imperative to study possible biochemical processes underlying allelopathy of some on other plants. In present study, investigation was carried out phytochemical composition aqueous and methanolic extracts <em>Tithonia diversifolia</em> their allelopathic effect growth <em>Vigna...

Aims: Pterocarpus osun and soyauxii seeds lectins were subjected to various chemical modifications in order detect the amino acid residues involved their hemagglutinating sugar-binding activities. Methodology: The purified using salt precipitation size exclusion chromatography. Hemagglutinating activity sugar specificity of also established. Chemical modification arginine was done phenylglyoxal hydrate, 5,5- dithiobis-(2-nitrobenzoic acid) (DTNB) used modify cysteine. Phenylmethylsulfonyl...

In Nigeria, Asystasia vogeliana leaf is used for treatment and management of many human illnesses. The research explored the antioxidant, cytotoxicity genotoxicity effects methanol extract (AVLME), in order to establish its anti-malignant potential. Fine powder air-dried plant leaves was produced by pulverization. It extracted using methanol. filtrate concentrated dryness on a rotavap at 40ºC. Antioxidant activity assessed Ferric reducing antioxidant potential (FRAP),...

Abstract Natural products, such as enzymatic hydrolysates and bioactive peptides from dietary sources, are safe alternatives to synthetic compounds linked various deleterious effects. The purpose of this study is determine the in vitro bioactivities (antioxidant anti-inflammatory activities) Garcinia kola seeds (GKPHs) at different enzyme (pepsin)-substrate ratios. G. protein, isolated by alkaline solubilization acid precipitation, was hydrolyzed with pepsin varying enzyme–substrate (E:S)...

The study investigated the presence of lectin in seeds Pterocarpus soyauxii, purified and determined its physicochemical properties as well antioxidant potential. Purification P. soyauxii (PSSL) was accomplished by ammonium sulphate precipitation gel filtration on Sephadex G-100, hemagglutination assay serial dilution DPPH radical scavenging, metal chelating lipid peroxidation inhibition assays were employed to evaluate PSSL agglutinated erythrocytes from human blood group ABO, rabbit rat....

The study investigated the antioxidant, hemagglutinating and larvicidal activities of protein isolates from seeds two underutilized plant species (Pterocarpus osun Bosquiea angolensis) in other to establish their medicinal value. antioxidant power was assessed by employing DPPH radical scavenging assay, ferric reducing assay total capacity (phosphomolybdenum method). Radical ability extracts were found be concentration dependent with IC50 value 0.58 ± 0.05 mg mL-1 3.98 0.28 for P. B....

Journal of Pharmaceutical Research International (ISSN: 2456-9119) is dedicated to publish high quality papers in all areas pharmaceutical Science including drugs, community pharmacy, hospital clinical compounding consultant internet veterinary nuclear military pharmacy informatics, pharmaceutics, medicinal chemistry, pharmacognosy, pharmacotherapy, pharmacodynamics, pharmacokinetics, pharmacology, neuropharmacology, psychopharmacology, pharmacogenetics, pharmacogenomics,...

Objective: This study was carried out to purify and characterize a carbohydrate-binding cell-agglutinating protein, lectin, from Pterocarpus osun seeds also evaluate its antioxidant antibacterial potential.
 Methods: Isolation purification of the lectin were done by ammonium sulphate precipitation size exclusion chromatography on Sephadex G-100. Physicochemical properties determined activity evaluated DPPH radical scavenging, lipid peroxidation inhibition ferric reducing potential...

Objectives: The study was designed to screen for the phytochemical constituents, and investigate antioxidant, antibacterial, anti-inflammatory activities of Leucaena leucocephala fruit pods methanolic extract (LLFPME).
 Materials Methods: Matured dried were pulverized extracted with methanol using maceration method. filtrate concentrated in a rotary evaporator under reduced pressure obtain powdery form termed LLFPME. LLFPME tested presence phytochemicals TLC GC-MS technique. phenolic...

Recently, there has been more attention on the bioactivity of phytochemicals and other metabolites in agro-food by-products for management oxidative stress–related conditions. Hence, objective this paper is to assess nutraceuticals potential by investigating antioxidant α-amylase inhibitory potentials seed coat protein isolates from four melon species namely: Citrullus colocynthis, mucosospermus, Cucumeropsis mannii Lagenaria siceraria using appropriate standard methods. At highest...

Majority of the tradomedicinal uses Asystasia vogeliana lack scientific backing. Therefore, this study sought to investigate phytochemical constituents and toxicological potentials methanolic leaf extract in Swiss albino mice. Air-dried pulverized leaves (1248 g) were extracted with methanol for 48 h. The was concentrated using rotary evaporator obtain crude (99.8 g). screened phytochemicalconstituents partitioned n-exane dichloromethane (DCM). two fractions subjected GC-MS analysis. Acute...

Study’s Excerpt This study is on inhibition of gentamicin-induced neprototoxicity in rats through the antioxidant and neproprotective qualities dichloromethane fraction (DCMF) methanol extract (ME) from root bark Garcinia kola. The extracts are abundant phytochemicals (specifically phenolics flavonoids), which bioactively restored disrupted equilibrium. reveals potential as good natural-source remedies to stop drug-induced kidney damage. Full Abstract investigated nephroprotective effects...

This study determined the degree of hydrolysis Mormyrus rume muscle proteins by digestive proteases and antioxidant potential hydrolysates produced was evaluated. The were extracted with distilled water adjusted to neutral pH. used include trypsin, chymotrypsin pepsin using trichloroacetic acid precipitation method. antioxidative activities evaluated different in-vitro assay methods such as 1,1-diphenyl-2- picrylhydrazyl (DPPH) radical scavenging, hydrogen peroxide metal chelating capacity...

Objectives: In this research, Dioscorea preussii root tuber haemagglutinin was purified and its physicochemical properties determined.The antioxidative prowess anti-haemolytic activities of the were also investigated.Material Methods: Hemagglutinating assay used to detect presence lectin in Phosphate Buffer Saline (PBS) extract D. tuber.The using ammonium sulphate fractionation molecular sieve chromatography.Optimum pH temperature determined.So also, antioxidant activity assessed DPPH...

Lately, there has been increased interest in bioactivity of antioxidant soluble proteins and other compounds agro-food by-products for the treatment oxidative stress–related conditions. In this study, from seed coats four melon species—Citrullus colocynthis, Citrullus mucosospermus, Cucumeropsis manii, Lagenaria siceraria were assessed their α-amylase inhibition activities. Individual powdered extracted with phosphate-buffered saline. Each protein was precipitated ammonium sulphate salt,...

The kinetic and physicochemical properties of glycogen phosphorylase b from the breast muscle fruit bat, Eidolon helvum Kerr were investigated in order to obtain some information about possible physiological role enzyme meeting energy requirements bat either at initiation or during flight. Glycogen was purified apparent homogeneity with specific activity approximately 37 units/mg protein a yield 4 %. completely dependent on AMP for activity; hence it designated b. native subunit molecular...

Aim:This study aimed at exploring the hemagglutinating and antibacterial activities of crude lectin from fruit pulp Trilepisium madagascariense DC.Methods: Crude was obtained by maceration ammonium sulphate precipitation.Hemagglutinating activity assay carried out determining ability extract to agglutinate red blood cells, serial dilution done followed addition erythrocytes.The subjected evaluation for inhibition bacterial growth agar well diffusion method against fourteen human pathogenic...

The study was carried out to evaluate the antioxidant activity and α-amylase inhibitory potential of Sarotherodon galillaeus muscle protein hydrolysates. isolate hydrolysed with three digestive proteases namely trypsin, chymotrypsin pepsin. Degree hydrolysis determined. antioxidative hydrolysates investigated using DPPH radical scavenging, ferric reducing power, hydrogen peroxide scavenging metal chelating activity. ability inhibit sugar-hydrolysing enzyme also evaluated. Highest degree...