A5015396057- Cancer-related gene regulation
- Epigenetics and DNA Methylation
- Cancer Genomics and Diagnostics
- Genetic factors in colorectal cancer
- RNA modifications and cancer
- Ubiquitin and proteasome pathways
- Wnt/β-catenin signaling in development and cancer
- Kruppel-like factors research
- Immune cells in cancer
- GDF15 and Related Biomarkers
- Cancer-related molecular mechanisms research
- Intraperitoneal and Appendiceal Malignancies
- Cancer-related Molecular Pathways
- RNA Research and Splicing
- Colorectal Cancer Treatments and Studies
- Protein Degradation and Inhibitors
- Acute Myeloid Leukemia Research
- Nuclear Receptors and Signaling
- Pancreatic function and diabetes
- Cholangiocarcinoma and Gallbladder Cancer Studies
- Macrophage Migration Inhibitory Factor
- Testicular diseases and treatments
- Immune Response and Inflammation
- Nitric Oxide and Endothelin Effects
- SARS-CoV-2 detection and testing
The University of Tokyo
2016-2025
Tokyo University of Science
2018-2024
Human Genome Sciences (United States)
2024
Advanced Medical Research Institute
2011-2023
Japan Institute for Advanced Dentistry
2023
San-ikukai Hospital
2022
Tokyo Medical University
2016-2020
Tottori University Hospital
2019
The University of Texas Southwestern Medical Center
2018
Nihon University
2016
Removal of senescent cells (senolysis) has been proposed to be beneficial for improving age-associated pathologies, but the molecular pathways such senolytic activity have not yet emerged. Here, we identified glutaminase 1 (GLS1) as an essential gene survival human cells. The intracellular pH in was lowered by lysosomal membrane damage, and this induced kidney-type (KGA) expression. resulting enhanced glutaminolysis ammonia production, which neutralized lower improved Inhibition...
The effectiveness and safety of yokukansan (TJ-54), a traditional Japanese medicine (kampo) for the treatment behavioural psychological symptoms dementia (BPSD), were evaluated in 106 patients diagnosed as having Alzheimer's disease (AD) (including mixed-type dementia) or with Lewy bodies. Patients randomly assigned to group A (TJ-54 period I no II; each lasting 4 wk) B (no TJ-54 II). BPSD cognitive functions using Neuropsychiatric Inventory (NPI) Mini-Mental State Examination (MMSE),...
Abstract We previously identified SMYD3 as a histone methyltransferase and showed that its expression was elevated in colorectal, hepatocellular, breast carcinomas. In the investigation of activity SMYD3, we have found vascular endothelial growth factor receptor 1 (VEGFR1) also methylated by SMYD3. further residue at VEGFR1 lysine 831, which is located kinase domain conserved among orthologues. followed serine, some methylation targets methyltransferases. Furthermore, enhanced cells. These...
Abstract Somatic mutations of ASXL1 are frequently detected in age-related clonal hematopoiesis (CH). However, how drive CH remains elusive. Using knockin (KI) mice expressing a C-terminally truncated form ASXL1-mutant (ASXL1-MT), we examined the influence ASXL1-MT on physiological aging hematopoietic stem cells (HSCs). HSCs display competitive disadvantage after transplantation. Nevertheless, genetic mosaic mouse model, they acquire advantage during aging, recapitulating humans....
Abstract Hematopoietic stem cells (HSCs) exhibit considerable cell-intrinsic changes with age. Here, we present an integrated analysis of transcriptome and chromatin accessibility aged HSCs downstream progenitors. Alterations in preferentially take place aging, which gradually resolve differentiation. Differentially open accessible regions (open DARs) are enriched for enhancers show enrichment binding motifs the STAT, ATF, CNC family transcription factors that activated response to external...
Changes in the gut microbiome have pivotal roles pathogenesis of acute graft-versus-host disease (aGVHD) after allogenic haematopoietic cell transplantation (allo-HCT)
In fetal development, tissue interaction such as the interplay between blood vessel (BV) and epithelial is crucial for organogenesis. Here we recapitulate spatial arrangement liver portal vein to observe formation of intrahepatic bile ducts (BDs) from human induced pluripotent stem cells (hiPSC). We co-culture hiPSC-liver progenitors on artificial BV consisting immature smooth muscle endothelial cells, both derived hiPSCs. After 3 weeks, within hiPSC-BV-incorporated organoids (BVLO)...
Conjugated linoleic acids (CLAs), naturally occurring fatty in ruminant food products, have anti-tumorigenic and pro-apoptotic properties animal as well vitro models of cancer. However, the cellular mechanism has not been fully understood. NAG-1 (non-steroidal anti-inflammatory drug-activated gene-1) is induced by several dietary compounds belongs to a TGF-β superfamily gene associated with activities. The present study was performed elucidate molecular which CLA stimulates activity human...
Abstract Intrahepatic cholangiocarcinoma (ICC) is an aggressive malignancy with poor prognosis and its incidence increasing worldwide. Recently, several types of cells have been considered as the origin ICC, namely cholangiocytes, liver progenitor cells, hepatocytes. Here, we established a novel mouse model ICC by liver-specific Kras activation Pten deletion. An activating mutation in combination deletion was introduced embryonic hepatic bipotential (so-called hepatoblasts) mature...
Abstract Pseudomyxoma peritonei ( PMP ) is a rare disease exhibiting distinct clinical feature caused by cancerous cells that produce mucinous fluid in the abdominal cavity. s originate most frequently from appendix and less ovary. This can range benign to malignant, histologically, classified into two types: disseminated peritoneal adenomucinosis DPAM representing milder phenotype, adenocarcinomas PMCA aggressive phenotype. Although histological classification clinically useful,...
Wastewater-based epidemiology is expected to be able identify SARS-CoV-2 variants at an early stage via next-generation sequencing. In the present study, we developed a highly sensitive amplicon sequencing method targeting spike gene of SARS-CoV-2, which allows for viral genomes from wastewater containing low amount virus. Primers were designed amplify relatively long region (599 bp) around receptor-binding domain in gene, could distinguish initial major concern. To validate methodology,...
Maximizing the potential of human liver organoids (LOs) for modeling septic requires integration innate immune cells, particularly resident macrophage Kupffer cells. In this study, we present a strategy to generate LOs containing cells (KuLOs) by recapitulating fetal hematopoiesis using induced pluripotent stem cell (hiPSC)-derived erythro-myeloid progenitors (EMPs), origin tissue-resident macrophages, and hiPSC-derived LOs. Remarkably, actively promote EMP toward myeloid erythroid lineages....
Lysosomal stress due to the accumulation of nucleic acids (NAs) activates endosomal TLRs in macrophages. Here, we show that lysosomal RNA stress, caused by lack RNase T2, induces macrophage multiple organs such as spleen and liver through TLR13 activation microbiota-derived ribosomal RNAs. triggered emergency myelopoiesis, increasing number myeloid progenitors bone marrow spleen. Splenic macrophages continued proliferate mature into expressing anti-inflammatory cytokine IL-10. In liver,...
The signaling pathway of phosphatidylinositol 3-kinase (PI3K)/AKT, which is involved in cell survival, proliferation, and growth, has become a major focus targeting cancer therapeutics. Nonsteroidal anti-inflammatory drug-activated gene (NAG-1) was previously identified as induced by several anti-tumorigenic compounds including nonsteroidal drugs, peroxisome proliferator-activated receptor gamma ligands, dietary compounds. NAG-1 been shown to exhibit and/or pro-apoptotic activities vivo...
Modifications of histone tails are involved in the regulation a wide range biological processes including cell cycle, survival, division, and differentiation. Among modifications, methylation plays critical role cardiac skeletal muscle In our earlier studies, we found that SMYD3 has methyltransferase activity to H3 lysine 4, its up-regulation is tumorigenesis human colon, liver, breast. To clarify Smyd3 development, have studied expression patterns zebrafish embryos effect suppression on...
Maintenance of chromosomal ends (telomeres) directly contributes to cancer cell immortalization. The telomere protection enzymes belonging the tankyrase (Tnks) subfamily poly(ADP-ribose) polymerases (PARPs) have recently been shown also control transcriptional response secreted Wnt signaling molecules. Whereas Tnks inhibitors are currently being developed as therapeutic agents for targeting Wnt-related cancers and modulators in tissue-engineering agendas, their impact on length maintenance...