A5024818620- Bacterial Genetics and Biotechnology
- Legume Nitrogen Fixing Symbiosis
- Bacteriophages and microbial interactions
- Fungal and yeast genetics research
- Bacterial biofilms and quorum sensing
- Microbial Natural Products and Biosynthesis
- Cell Image Analysis Techniques
- RNA and protein synthesis mechanisms
- Plant-Microbe Interactions and Immunity
- Genomics and Phylogenetic Studies
- Molecular Biology Techniques and Applications
- Mass Spectrometry Techniques and Applications
- Advanced Proteomics Techniques and Applications
- Protist diversity and phylogeny
University of Basel
2019-2020
John Innes Centre
2012-2013
Norwich Research Park
2012-2013
Lund University
2008-2012
Time-lapse imaging of Streptomyces hyphae revealed foci the essential protein DivIVA at sites where lateral branches will emerge. Overexpression experiments showed that can trigger establishment new zones cell wall assembly, suggesting a key role in directing peptidoglycan synthesis and shape Streptomyces.
In cells that exhibit apical growth, mechanisms regulate cell polarity are crucial for determination of cellular shape and the adaptation growth to intrinsic extrinsic cues. Broadly conserved pathways control in eukaryotes, but less is known about polarly growing prokaryotes. An evolutionarily ancient form found filamentous bacteria Streptomyces , directed by a polarisome-like complex involving essential protein DivIVA. We report here this bacterial polarization machinery regulated...
Abstract Bacteria adapt their growth rate to metabolic status and environmental conditions by modulating the length of G1 period. Here we demonstrate that a gradual increase in concentration second messenger c-di-GMP determines precise gene expression during G1/S transition Caulobacter crescentus . We show stimulates kinase ShkA binding its central pseudo-receiver domain, activates TacA transcription factor, initiates G1/S-specific program leading cell morphogenesis S-phase entry. Activation...
Many filamentous organisms, such as fungi, grow by tip-extension and forming new branches behind the tips. A similar growth mode occurs in bacteria, including genus Streptomyces, although here our mechanistic understanding has been very limited. The Streptomyces protein DivIVA is a critical determinant of hyphal localizes foci at tips sites future branch development. However, how form was previously unknown. Here, we show experimentally that focus-formation involves novel mechanism which...
The filamentous bacterium Streptomyces coelicolor modulates polar growth and branching by phosphorylating the cytoskeletal protein DivIVA. Previous MALDI-TOF analysis of DivIVA showed that a large 7.2 kDa tryptic peptide was multiply phosphorylated. To aid localization phosphorylation sites, we introduced additional cleavage sites into DivIVA, resulting phosphopeptides were analyzed LC-MS/MS. Phosphopeptide isomers could be separated chromatographically, but because overlapping elution...
ABSTRACT Bacteria adapt their growth rate to metabolic status and environmental conditions by modulating the length of quiescent G1 period. But molecular mechanisms controlling exit from are poorly understood. Here we identify a key role for second messenger c-di-GMP, demonstrate that gradual increase in c-di-GMP concentration determines precise gene expression during G1/S Caulobacter crescentus . We show strongly stimulates kinase ShkA, activates TacA transcription factor, initiates...