A5035738242- Liver Disease Diagnosis and Treatment
- Redox biology and oxidative stress
- Advanced Nanomaterials in Catalysis
- Connexins and lens biology
- Heat shock proteins research
- Alcohol Consumption and Health Effects
- Nanoplatforms for cancer theranostics
- Bioactive Compounds and Antitumor Agents
- Protein Tyrosine Phosphatases
- Cancer, Hypoxia, and Metabolism
- Cell death mechanisms and regulation
- Research in Cotton Cultivation
- Photodynamic Therapy Research Studies
- Glycosylation and Glycoproteins Research
- Advanced biosensing and bioanalysis techniques
- Nicotinic Acetylcholine Receptors Study
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- CAR-T cell therapy research
- Mitochondrial Function and Pathology
- Nanocluster Synthesis and Applications
- Genomics, phytochemicals, and oxidative stress
- Skin Protection and Aging
- Eicosanoids and Hypertension Pharmacology
- Endoplasmic Reticulum Stress and Disease
- Curcumin's Biomedical Applications
Heinrich Heine University Düsseldorf
2005-2024
Düsseldorf University Hospital
2024
Institut d'Investigacions Biomèdiques de Barcelona
2012-2013
Consorci Institut D'Investigacions Biomediques August Pi I Sunyer
2010-2013
Centro de Investigación Biomédica en Red
2010-2013
Hospital Clínic de Barcelona
2013
Euskadiko Parke Teknologikoa
2012
University of Louisville
2007-2010
Consejo Superior de Investigaciones Científicas
2009
University of Hohenheim
2009
Aims: Melanoma is the most aggressive type of malignant skin cancer derived from uncontrolled proliferation melanocytes. cells possess a high potential to metastasize, and prognosis for advanced melanoma rather poor due its strong resistance conventional chemotherapeutics. Nanomaterials are at cutting edge rapidly developing area nanomedicine. The nanoparticles use as carrier in drug delivery infinite with novel applications constantly being tested. noncarrier cerium oxide (CNPs) promising...
Abstract The early stages of alcohol-induced liver injury involve chronic inflammation. Whereas mechanisms by which this effect is mediated are not completely understood, it hypothesized that enhanced sensitivity to circulating lipopolysaccharide (LPS) contributes process. It has recently been shown ethanol induces activation plasminogen activator inhibitor-1 (PAI-1). PAI-1 causes fibrin accumulation in inhibiting degradation (fibrinolysis). LPS also enhances activating the coagulation...
Plasminogen activator inhibitor-1 (PAI-1) is an acute phase protein that has been shown to play a role in experimental fibrosis caused by bile duct ligation (BDL) mice. However, its more severe models of hepatic (e.g., carbon tetrachloride; CCl(4)) not determined and important for extrapolation human disease. Wild-type or PAI-1 knockout mice were administered CCl(4) (1 ml/kg body wt ip) 2x/wk 4 wk. Plasma transaminase activity) histological Sirius red staining) indexes liver damage...
Recently, it has been published that cerium (Ce) oxide nanoparticles (CNP; nanoceria) are able to downregulate tumor invasion in cancer cell lines. Redox-active CNP exhibit both selective pro-oxidative and antioxidative properties, the first being responsible for impairment of growth invasion. A non-toxic even protective effect human dermal fibroblasts (HDF) already observed. However, on important parameters such as death, proliferation redox state cells needs further clarification. Here, we...
Studies in rodents suggest that the adipocytokine resistin causes insulin resistance via impairing normal signaling. However, humans, may play a more important role inflammation than resistance. Whether contributes to is unclear. Therefore, purpose of present study was determine effect exposure on basal and stimulated [lipopolysaccharide (LPS)] inflammatory response mouse liver vivo. Resistin alone had no major effects hepatic expression insulin-responsive genes, either presence or absence...
It is well known that ethanol preexposure sensitizes the liver to LPS hepatotoxicity. The mechanisms by which enhances LPS-induced injury are not completely elucidated but involve an enhanced inflammatory response. Ethanol exposure also increases metabolic rate of liver, and this effect on mediated, at least in part, sympathetic hormone, epinephrine. However, whether or nervous system contributes sensitizing damage has been determined. purpose study was therefore test hypotheses 1)...
Cerium (Ce) oxide nanoparticles (CNP; nanoceria) are reported to have cytotoxic effects on certain cancerous cell lines, while at the same concentration they show no cytotoxicity normal (healthy) cells. Redox-active CNP exhibit both selective prooxidative as well antioxidative properties. The former is proposed be responsible for impairment of tumor growth and invasion latter rescuing cells from reactive oxygen species (ROS)-induced damage. Here we address possible underlying mechanisms in a...
Singlet oxygen, an electronically excited form of molecular is a primary mediator the activation stress-activated protein kinases elicited by ultraviolet A (UVA; 320-400 nm). Here, effects singlet oxygen (1O2) on extracellular signal-regulated kinase (ERK) 1/2 and Akt/protein B pathways were analyzed in human dermal fibroblasts. While basal ERK phosphorylation was lowered cells exposed to either 1O2, UVA or photodynamic treatment, Akt moderately activated photochemically generated 1O2...
Singlet oxygen (1O2), an electronically excited form of molecular oxygen, is a mediator biological effects ultraviolet A radiation, stimulating signaling cascades in human cells. We demonstrate here that 1O2 generated by photosensitization or thermodecomposition 3,3′-(1,4-naphthylidene)dipropionate-1,4-endoperoxide inactivates isolated protein tyrosine phosphatases (PTPases). PTPase activities PTP1B CD45 were abolished low concentrations 1O2, but largely restored post-treatment with...
Malignant melanoma is an aggressive type of cancer and the deadliest form skin cancer. Even though enormous efforts have been undertaken, in particular treatment options against metastasizing are challenging prognosis generally poor. A novel therapeutical approach application secondary plant constituents occurring food products. Herein, effect dietary chalcone cardamonin, inter alia found Alpinia species, was tested using human malignant cells. These data were compared to cardamonin treated...
Background Hypoxia-mediated HIF-1α stabilization and NF-κB activation play a key role in carcinogenesis by fostering cancer cell survival, angiogenesis tumor invasion. Gangliosides are integral components of biological membranes with an increasingly recognized as signaling intermediates. In particular, ganglioside GD3 has been characterized proapoptotic lipid effector promoting death suppression survival pathways. Thus, our aim was to analyze the hypoxia susceptibility hepatocarcinoma cells...
Activation of ErbB receptor tyrosine kinases triggers multiple signaling pathways that regulate cellular proliferation and survival. We here demonstrate ErbB2 is activated via the epidermal growth factor (EGFR) upon exposure cultured human keratinocytes to 2‐methyl‐1,4‐naphthoquinone (menadione). Both EGFR are shown be regulated by protein phosphatases inhibited menadione, giving rise hypothesis phosphatase inhibition menadione may result in a net activation an enhanced phosphorylation....
Abstract A major challenge in current cancer therapy is still the treatment of metastatic melanomas skin. BH3 mimetics represent a novel group substances inducing apoptosis. In this study, we investigated cytotoxic effect (±) gossypol (GP), natural compound from cotton seed, on A375 melanoma cells and underlying biochemical mechanisms. To prevent undesired side effects due to toxicity normal (healthy) cells, concentrations only toxic for tumor have been elaborated. Viability assays were...
Background Malignant melanoma is the most aggressive form of skin cancer with a rather poor prognosis. Standard chemotherapy often results in severe side effects on normal (healthy) cells finally being difficult to tolerate for patients. Shown by us earlier, cerium oxide nanoparticles (CNP, nanoceria) selectively killed A375 while not cytotoxic at identical concentrations non-cancerous cells. In conclusion, redox-active CNP exhibited both prooxidative as well antioxidative properties. that...
Abstract Exposure of rat liver epithelial cells to doxorubicin, an anthraquinone derivative widely employed in cancer chemotherapy, led a dose-dependent decrease gap junctional intercellular communication (GJC). Gap junctions are clusters inter-cellular channels consisting connexins, the major connexin used being connexin-43 (Cx43). Doxorubicin-induced loss GJC was mediated by activation extracellular signal-regulated kinase (ERK)-1 and ERK-2, as demonstrated using inhibitors ERK activation....