A5042504464- Skin Protection and Aging
- Advanced Nanomaterials in Catalysis
- Antioxidant Activity and Oxidative Stress
- melanin and skin pigmentation
- Nanoplatforms for cancer theranostics
- Selenium in Biological Systems
- Redox biology and oxidative stress
- Telomeres, Telomerase, and Senescence
- Heme Oxygenase-1 and Carbon Monoxide
- Trace Elements in Health
- Photodynamic Therapy Research Studies
- Wound Healing and Treatments
- Circadian rhythm and melatonin
- Genomics, phytochemicals, and oxidative stress
- Cancer, Hypoxia, and Metabolism
- Heavy Metal Exposure and Toxicity
- Medical and Biological Ozone Research
- Protease and Inhibitor Mechanisms
- Dermatologic Treatments and Research
- Cancer Cells and Metastasis
- Nanoparticle-Based Drug Delivery
- Angiogenesis and VEGF in Cancer
- Psoriasis: Treatment and Pathogenesis
- Dermatology and Skin Diseases
- Nanocluster Synthesis and Applications
Heinrich Heine University Düsseldorf
2013-2024
Düsseldorf University Hospital
2024
Griffith University
2019
University of Cologne
1996-2004
St. Josef-Hospital
1997
University of Hohenheim
1994
Reactive oxygen species (ROS) are important second messengers for the induction of several genes in a variety physiological and pathological conditions. Ultraviolet B (UVB) irradiation has recently been shown to generate lipid peroxidation products hydroxyl radicals (HO⋅) with detrimental long term effects like cancer formation premature aging skin. Here, we addressed question whether ferric/ferrous iron via generation ROS may mediate UVB response, finally leading connective tissue...
Aims: Melanoma is the most aggressive type of malignant skin cancer derived from uncontrolled proliferation melanocytes. cells possess a high potential to metastasize, and prognosis for advanced melanoma rather poor due its strong resistance conventional chemotherapeutics. Nanomaterials are at cutting edge rapidly developing area nanomedicine. The nanoparticles use as carrier in drug delivery infinite with novel applications constantly being tested. noncarrier cerium oxide (CNPs) promising...
Abstract Nanotechnology is becoming an important field of biomedical and clinical research the application nanoparticles in disease may offer promising advances treatment many diseases, especially cancer. Malignant melanoma one most aggressive forms cancer its incidence rapidly increasing. Redox-active cerium oxide (CNP) are known to exhibit significant antitumor activity cells derived from human skin tumors vitro vivo, whereas CNP nontoxic beyond that even protective (antioxidative) normal,...
The incidence of numerous types cancer has been increasing over recent years, representing the second-most frequent cause death after cardiovascular diseases. Even though, number effective anticancer drugs is as well, a large patients suffer from severe side effects (e.g., cardiomyopathies) caused by these drugs. This adversely affects patients’ well-being and quality life. On molecular level, tumor cells that survive treatment modalities can become chemotherapy-resistant. In addition,...
A major fraction of the essential trace element selenium circulating in human blood plasma is present as selenoprotein P (SeP). As SeP associates with endothelial membranes, participation selenium-mediated protection against oxidative damage was investigated, using cell line Ea.hy926 a model system. Hepatocyte-derived prevented tert-butylhydroperoxide (t-BHP)-induced death cells similar manner did sodium selenite, counteracting t-BHP-induced loss cellular membrane integrity. Protection...
Ultraviolet (UV) A irradiation of human dermal fibroblasts elicits an increase in specific mRNA amounts and bioactivities the cytokines IL‐1α, IL‐1β, IL‐6. These effects are enhanced deuterium oxide‐based medium diminished presence non‐toxic concentrations sodium azide. Furthermore, generating singlet oxygen outside cells by rose bengal‐coated resin particles with visible light ( λ >450 nm) results induction interstitial collagenase, IL‐1 IL‐6, similar to response observed UVA...
Myofibroblasts, pivotal for tumor progression, populate the microecosystem of reactive stroma. Using an in vitro tumor-stroma model skin carcinogenesis, we report here that tumor-cell-derived transforming growth factor beta1 (TGFbeta1) initiates oxygen species-dependent expression alpha-smooth muscle actin, a biomarker myofibroblastic cells belonging to group late-responsive genes. Moreover, protein kinase C (PKC) is involved lipid hydroperoxide-triggered molecular events underlying...
Abstract— The wavelength dependence for the regulation of two major matrix‐metalloproteinases, interstitial collagenase (MMP‐1) and stromelysin‐1 (MMP‐3), their inhibitor, tissue inhibitor metalloproteinases (TIMP‐1), was studied in human dermal fibroblasts vitro. Monochromatic irradiation at 302, 307, 312 317 nm with intensities ranging from 20 to 300 J/m 2 increased MMP‐1 MMP‐3 mRNA steady‐state levels secretion corresponding proteins up 4.4‐fold, whereas almost no increase observed...
Astrocyte dysfunction is a primary factor in hepatic encephalopathy (HE) impairing neuronal activity under hyperammonemia. In particular, the early events causing ammonia-induced toxicity to astrocytes are not well understood. Using established cellular HE models, we show that mitochondria rapidly undergo fragmentation reversible manner upon Further, our analyses, within timescale of minutes, mitochondrial respiration and glycolysis were hampered, which occurred pH-independent manner....
Ultraviolet B (UVB) irradiation has been shown to stimulate the expression of matrix-degrading metalloproteinases via generation DNA damage and/or reactive oxygen species. Matrix-degrading promote UVB-triggered detrimental long term effects like cancer formation and premature skin aging. Here, we were interested in identifying components signal transduction pathway that causally link UVB-mediated induction metalloproteinase (MMP)-1/interstitial collagenase MMP-3/stromelysin-1 human dermal...
Abstract Human skin undergoes morphological, biochemical and functional modifications during the ageing process. This study was designed to produce a 3‐dimensional (3D) equivalent in vitro reflecting some aspects of vivo aged skin. Reconstructed generated by co‐culturing fibroblasts keratinocytes on collagen–glycosaminoglycan–chitosan scaffold, induced exposition Mitomycin‐C ( MMC ). Recently published data showed that treatment resulted drug‐induced accelerated senescence DIAS ) human...
Phospholipid-hydroperoxide glutathione peroxidase (PHGPx) exhibits high specific activity in reducing phosphatidylcholine hydroperoxides (PCOOHs) and thus may play a central role protecting the skin against UV irradiation-triggered detrimental long term effects like cancer formation premature aging. Here we addressed of PHGPx protection irradiation-induced expression matrix metalloproteinase-1 (MMP-1). For this purpose, created human dermal fibroblast cell lines overexpressing PHGPx....