A5038105964- Insect and Arachnid Ecology and Behavior
- Insect and Pesticide Research
- Plant and animal studies
- Invertebrate Immune Response Mechanisms
- Mosquito-borne diseases and control
- Insect Resistance and Genetics
- Insect symbiosis and bacterial influences
- Plant Virus Research Studies
- Neurobiology and Insect Physiology Research
- Viral Infectious Diseases and Gene Expression in Insects
- Viral Infections and Vectors
- Insect-Plant Interactions and Control
National Taiwan University
2020-2025
RNA interference (RNAi) is a promising gene-silencing technique for pest control that targets essential genes. We assessed the potential of double-stranded (dsRNA) and small interfering (siRNA) to silence mesh or iap genes in midguts Spodoptera litura larvae. Despite theoretical promise RNAi approaches, our findings revealed dsRNA did not induce significant gene silencing impact larval growth, whereas siRNA exhibited clear insecticidal effects, likely by disrupting intestinal osmoregulation...
ABSTRACT Efficient and economical purification methods are crucial for the commercial production of recombinant proteins with biomedical applications. In this study, we developed an affinity chromatography system that leverages polysaccharide‐binding properties galectin‐1 (GAL1) as a protein tag. The known GAL1‐binding material, chitin, was used matrix. Melittin (MELT), bee venom peptide its antimicrobial anti‐inflammatory potential, chosen to validate system. GAL1–MELT fusion expressed in...
Abstract To avoid inducing immune and physiological responses in insect hosts, parasitoid wasps have developed several mechanisms to inhibit them during parasitism, including the production of venom, specialized wasp cells, symbioses with polydnaviruses (PDVs). These alter host physiology give offspring a greater chance survival. However, molecular for most these alterations remain unclear. In present study, we applied next-generation sequencing analysis identified miRNAs that were encoded...
Abstract Sufficient energy supply to the host immune system is important for resisting pathogens. Therefore, during pathogen infection, metabolism reassigned from storage, growth, and development system. Previous studies in Drosophila melanogaster have demonstrated that systemic metabolic switching upon an challenge activated by extracellular adenosine signaling, modulating carbohydrate mobilization redistributing hemocytes. In present study, we discovered symbiotic virus (SmBV) of...
Plants and pollinators are mutually beneficial: plants provide nectar as a food source in return their pollen is disseminated by such honeybees. Some secrete chemicals to deter herbivores protective measure, among which caffeine, naturally occurring, bitter tasting, pharmacologically active secondary compound. It can be found low concentrations the nectars of some such, when consume nectar, they also take small amounts caffeine. Whilst caffeine has been indicated an antioxidant both mammals...
Baculoviruses Autographa californica multicapsid nucleopolyhedrovirus (AcMNPV) and Bombyx mori (BmNPV) have highly similar genome sequences but exhibit no overlap in their host range. After baculovirus infects nonpermissive larvae (e.g., AcMNPV infecting B. or BmNPV Spodoptera litura), we found that stored carbohydrates, including hemolymph trehalose fat body glycogen, are rapidly transformed into glucose; enzymes involved glycolysis the TCA cycle upregulated produce more ATP; adenosine...
Impairment in the learning/memory behavior of bees is responsible for massive disappearance bee populations and its consequent agricultural economic losses. Such impairment might be because o both pesticide exposure pathogen infection, with a key contributor deformed wing virus (DWV). The present study found that sodium butyrate (NaB) significantly increased survival reversed DWV-infected bees. A next-generation sequencing analysis showed NaB affected expression genes involved glycolytic...