Honeybees play a crucial role as agricultural pollinators and are frequently exposed to various pollutants, including pesticides. In this study, we aimed evaluate the toxicity of lambda-cyhalothrin (LCY) spinetoram (SPI) in honey bee larvae reared vitro through single (acute) repeated (chronic) exposure. The acute LD50 values for LCY SPI were 0.058 (0.051–0.066) 0.026 (0.01–0.045) μg a.i./larva, respectively. chronic exposure, 0.040 (0.033–0.046) 0.017 (0.014–0.019) no-observed-effect dose...

Recently, cyantraniliprole (CYA) and sulfoxaflor (SUL) have been considered as alternatives to neonicotinoid insecticides. In this study, we evaluated the acute chronic toxicities of CYA SUL on honey bee (Apis mellifera L.) larvae reared in vitro.In toxicity test, following test doses were used determine median lethal dose (LD50 ): 0.007, 0.014, 0.028, 0.056 0.112 μg larva-1 ; 2.5, 5, 10, 20 40 . LD50 : 0.00512, 0.0128, 0.032, 0.08 0.2 0.0625, 0.125, 0.25, 0.5 1.0 The values 0.047 11.404 ,...

Organophosphate pesticides (OPPs) are one of the most widely used insecticides. OPPs exert their neurotoxic effects by inhibiting acetylcholine esterase (AChE). Most gross developmental abnormalities observed in OPP-treated fish, on other hand, may not be explained solely AChE inhibition. To understand overall molecular mechanisms involved OPP toxicity, we zebrafish (ZF) model. We exposed ZF embryos to an OPP, phosmet, for 96 h, and then analyzed performed whole transcriptome analysis....

Thiram, an oxidized dimer of dithiocarbamate, has fungicidal and ectoparasiticidal roles. This study aimed to determine the effects thiram on development zebrafish (ZF) embryos. The developmental toxicity test was performed in accordance with OECD 236 guidelines, ZF embryos were subjected several concentrations a DMSO (0.01%) control. Subsequently, embryo mortalities anomalies evaluated at different hours post fertilization (hpf). Thiram highly toxic ZF, calculated median lethal (LC50) 48 96...

Thiram, a dithiocarbamate fungicide, is used for the treatment of various fungal infections affecting crops and ornamentals. However, thiram-associated toxicity has been reported in animals, including fish, underlying molecular mechanisms are unclear. Herein, we employed zebrafish (ZF) to gain further insights into thiram toxicity-associated mechanisms. We studied developmental abnormalities performed whole-transcriptome analysis ZF embryos exposed 96 h. Embryos 4.0 μg/L exhibited several...

Abstract. Recently, rainwater composition affected by atmospheric pollutants has been the topic of intense study in East Asia because its adverse environmental and human health effects. In present study, chemical organic compounds were investigated from June to December 2012 at Gwangju Korea. The aim this is determine seasonal variation identify possible sources inorganic compounds. volume-weighted mean pH ranged 3.83 8.90 with an average 5.78. Of samples, 50 % had values below 5.6....

This study investigated the transformation of dissolved organic matter (DOM) in a free-water surface flow constructed wetland. Pyrolysis gas chromatography-mass spectrometry (Py-GC/MS) coupled with preparative high-performance liquid chromatography (prep-HPLC) was used to analyze compositions biopolymers (polysaccharides, amino sugars, proteins, polyhydroxy aromatics, lipids and lignin) DOM according molecular size at three sampling points water flow: inflow, midflow, outflow. The prep-HPLC...

Bees are important pollinators in agriculture. The bee population has recently begun to decline possibly due pesticides. gut microbiota strongly influences the health of bees. bees is composed distinct members belonging selective taxa. Chemicals like pesticides can alter microbiota. present study investigated effect carbaryl on honey bees, which had come contact with rapeseed plants (Brassica napus) sprayed wettable powder during brood test under semi-field condition. Molecular techniques...

Abstract Wastewater reclamation processes, including membrane bio reactor (MBR) and nanofiltration (NF) membrane, have been built, those processes were independently operated, under recycling conditions, to compare the performances, with respect contaminants removal fouling minimization. The hydrophilic fractions of organic matter more effectively removed than hydrophobic fraction through system due microbial activities in MBR, as measured using three dimensional fl uorescence excitation...

Etridiazole (EDZ) is a thiadiazole-containing fungicide commonly used to control Pythium and Phytophthora spp. Although previous studies have shown that EDZ teratogenic, the exact molecular mechanisms underlying its toxicity remain unknown. In this study, zebrafish (Danio rerio; ZF) model was explore pathways associated with toxicity. The whole transcriptome of ZF embryos exposed 96 h analyzed, along developmental abnormalities. EDZ-induced malformations were primarily related eyes, heart,...

The biological reduction of both nitrate and sulfate in constructed wetlands, connected to a wastewater treatment plant, was investigated. Three different macrophytes, including Acorus, Nuphar, Typha plants, were dominant the free surface flow wetlands; effluent flows through Nuphar plants ponds order. Nitrate substantially reduced throughout while significantly only wetland, under anoxic conditions. A real-time polymerase chain reaction (RT-PCR) technique performed identify quantify...

토양 중 잔류농약의 작물에 대한 흡수이행 평가 및 잔류허용기준 초과 가능성을 구명하기 위하여 지난 10여 년간 농경지 잔류농약 모니터링 결과 검출빈도와 검출농도가 높은 살충제 endosulfan, chlorfenapyr, chlorpyrifos, ethoprofos와 살균제 isoprothiolane, procymidone, fthalide 등 7종을 선발하고, 이들 농약을 토양에 혼화 처리 후 포트 포장에서 잔류농도의 변화를 조사하였다. 또한 시험약제가 처리된 엽채류(배추, 상추, 시금치), 근채류(무, 감자, 당근) 과채류(오이, 고추, 가지, 토마토) 채소 등을 재배하여 수확기 토양중 작물체로의 정도를 포트에서 양토중 엽채류의 흡수비는 ethoprophos > chlorpyrifos ≥ endosulfan 순으로 낮았으며, 사양토에서는 순이었다. 작물별 흡수는 대체로 배추 >시금치 >상추 적었다. 노지 근채류 재배지 반감기는 양토에서 chlorfenapyr (13일)...

Abstract Etridiazole (EDZ), a thiadiazole-containing toxic chemical, is widely used as fungicide. Regular usage of EDZ may reach and contaminate water bodies, but its adverse effects on aquatic vertebrates have not been well studied. Therefore, the present study aimed to evaluate harmful using zebrafish (ZF) ( Danio rerio ) embryos. ZF embryos were treated with 3.75, 7.5, 15, 30, 60 mg/L EDZ. Subsequently, mortality developmental toxicities quantified at 24, 48, 72, 96 h post fertilization...

Pesticide toxicity is a global concern for honeybee populations, and understanding these effects at the molecular level critical. This study analyzed transcriptome of honeybees larval adult stages after chronic exposure to sublethal dose (0.0017 µg a.i./larva) spinetoram (SPI) during phase. Four groups were used: acetone-treated larvae (ATL), adults (ATAs), SPI-treated (STL), (STAs). In total, 5719 differentially expressed genes (DEGs) identified ATL vs. ATAs, 5754 STL STAs, 273 STL, 203...

Abstract. Alpha, beta, and gamma proteobacteria comprise approximately 68, 16, 7% of all identified bacteria. In this study, bacterial communities that had fouled polyvinylidene fluoride microfiltration membranes, which are used for drinking water treatment, over an 18 month period were analyzed using the 16s rRNA gene clone library method. The alpha, composed mainly Bradyrhizobium Rhodopseudomonas, Ralstonia, Legionella, respectively. presence a relatively high amount alpha was due to...

Rearing honey bee larvae in vitro is an ideal method to study larval diseases or the toxicity of pesticides on under standardized conditions. However, recent studies reported that a horizontal position may cause deformation emerged bees. Accordingly, purpose this was evaluate emergence and rates (Apis mellifera ligustica) reared vertical positions. The conducted same laboratory conditions with three experimental groups, non-capped capped plates plates. our results demonstrated exhibited...

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