A5016058668- Neuroscience and Neuropharmacology Research
- Neural dynamics and brain function
- Neuroscience and Neural Engineering
- Functional Brain Connectivity Studies
- Genetic Neurodegenerative Diseases
- Photoreceptor and optogenetics research
- Mitochondrial Function and Pathology
- Neurotransmitter Receptor Influence on Behavior
- Advanced Memory and Neural Computing
- Advanced MRI Techniques and Applications
- Ion channel regulation and function
- Neonatal and fetal brain pathology
- Zebrafish Biomedical Research Applications
- Traumatic Brain Injury Research
- Visual perception and processing mechanisms
- CCD and CMOS Imaging Sensors
- Cell Adhesion Molecules Research
- Neuroscience of respiration and sleep
- Obsessive-Compulsive Spectrum Disorders
- Electrochemical Analysis and Applications
- Neuroendocrine regulation and behavior
- Memory and Neural Mechanisms
- Neurological disorders and treatments
- Amino Acid Enzymes and Metabolism
- Traumatic Brain Injury and Neurovascular Disturbances
University of Minnesota
2015-2025
Twin Cities Orthopedics
2019-2024
Center for Neurosciences
2023
Neural computations occurring simultaneously in multiple cerebral cortical regions are critical for mediating behaviors. Progress has been made understanding how neural activity specific contributes to behavior. However, there is a lack of tools that allow simultaneous monitoring and perturbing from regions. We engineered 'See-Shells'-digitally designed, morphologically realistic, transparent polymer skulls long-term (>300 days) optical access 45 mm
Motor behavior results in complex exchanges of motor and sensory information across cortical regions. Therefore, fully understanding the cerebral cortex's role requires a mesoscopic-level description regions engaged, their functional interactions, how these interactions change with behavioral state. Mesoscopic Ca2+ imaging through transparent polymer skulls mice reveals elevated activation dorsal cortex during locomotion. Using correlations between time series fluorescence from 28 (nodes)...
Abstract The stability and flexibility of the functional parcellation cerebral cortex is fundamental to how familiar novel information both represented stored. We leveraged new advances in Ca2+ sensors microscopy understand dynamics segmentation dorsal cortex. performed wide-field imaging head-fixed mice used spatial independent component analysis (ICA) identify sources fluorescence. data were evaluated over multiple timescales discrete behaviors including resting, walking, grooming. When...
Electrophysiology and optical imaging provide complementary neural sensing capabilities - electrophysiological recordings have high temporal resolution, while allows recording of genetically-defined populations at spatial resolution. Combining these two modalities for simultaneous large-scale, multimodal activity across multiple brain regions can be very powerful. Here, transparent, inkjet-printed electrode arrays with outstanding electrical properties are seamlessly integrated...
>2.5 million individuals in the United States suffer mild traumatic brain injuries (mTBI) annually. Mild TBI is characterized by a brief period of altered consciousness, without objective findings anatomic injury on clinical imaging or physical deficit examination. Nevertheless, subset mTBI patients experience persistent subjective symptoms and repeated can lead to quantifiable neurological deficits, suggesting that each alters neurophysiology deleterious manner not detected using current...
Spinocerebellar Ataxia Type 8 (SCA8) is an inherited neurodegenerative disease caused by a bidirectionally expressed CTG●CAG expansion mutation in the ATXN-8 and ATXN8-OS genes. While SCA8 patients have motor abnormalities, may also exhibit psychiatric symptoms cognitive dysfunction. It difficult to elucidate how alters brain function areas with little or no degeneration producing both symptoms. Using transparent polymer skulls CNS-wide GCaMP6f expression, we studied neocortical networks...
The Ca 2+ channelopathies caused by mutations of the CACNA1A gene that encodes pore-forming subunit human v 2.1 (P/Q-type) voltage-gated channel include episodic ataxia type 2 (EA2). Although, in EA2 emphasis has been on cerebellar dysfunction, patients also exhibit episodic, nonmotoric abnormalities involving cerebral cortex. This study demonstrates low-frequency oscillations (LFOs) throughout cortex tottering ( tg / ) mice, a widely used model EA2. Ranging between 0.035 and 0.11 Hz, LFOs...
Summary Decision-making during freely moving behaviors involves complex interactions among many cortical and subcortical regions. However, the spatiotemporal coordination across regions to generate a decision is less understood. Using head-mounted widefield microscope, cortex-wide calcium dynamics were recorded in mice expressing GCaMP7f as they navigated an 8-maze using two paradigms. The first was alternating pattern that required short term memory of previous trial make correct second...
Myotonic dystrophy (DM) is a progressive, multisystem disorder affecting skeletal muscle, heart, and central nervous system. In both DM1 DM2, microsatellite expansions of CUG CCUG RNA repeats, respectively, accumulate disrupt functions alternative splicing factors, including muscleblind (MBNL) proteins. Grey matter loss white changes, the corpus callosum, likely underlie cognitive executive function deficits in DM patients. However, little known how cerebral cortical circuitry changes DM....
ABSTRACT Motor behavior results in widespread activation of the cerebral cortex. Therefore, fully understanding cortex’s role motor requires a mesoscopic level description engaged cortical regions and their functional interactions. Mesoscopic imaging Ca 2+ fluorescence through transparent polymer skulls implanted on transgenic Thy1-GCaMP6f mice reveals cortex during locomotion, including not only primary somatosensory but also secondary motor, retrosplenial, visual cortices. Using time...
ABSTRACT Neural computations occurring simultaneously in multiple cerebral cortical regions are critical for mediating cognition, perception and sensorimotor behaviors. Enormous progress has been made understanding how neural activity specific contributes to behavior. However, there is a lack of tools that allow simultaneous monitoring perturbing from regions. To fill this need, we have engineered “See-Shells” – digitally designed, morphologically realistic, transparent polymer skulls...
Episodic Ataxia Type 2 (EA2) is a rare neurological disorder caused by mutation in the CACNA1A gene, encoding P/Q-type voltage-gated Ca2+ channel important for neurotransmitter release. Patients with this channelopathy exhibit both cerebellar and cerebral pathologies, suggesting condition affects regions. The tottering (tg/tg) mouse most commonly used EA2 model due to an orthologous cacna1a gene. tg/tg has three prominent behavioral phenotypes: dramatic episodic dystonia; absence seizures...
Spinocerebellar Ataxia Type 8 (SCA8) is an inherited neurodegenerative disease caused by a bidirectionally expressed CTG●CAG expansion mutation in the
ABSTRACT The stability and flexibility of the functional parcellation cerebral cortex is fundamental to how familiar novel information both represented stored. We leveraged new advances in Ca 2+ sensors microscopy understand dynamics segmentation dorsal cortex. performed wide-field imaging head-fixed mice used spatial Independent Component Analysis (ICA) identify independent sources fluorescence. data were evaluated over multiple timescales discrete behaviors including resting, walking,...
Abstract The physiologic basis underlying the long-term consequences of repetitive, mild traumatic brain injury (mTBI) remains poorly understood. Mild often results in brief loss consciousness, impaired attention and concentration, memory problems, impulsivity, headache, without objective findings on clinical imaging or examination. effects mTBI can persist become cumulative with repetitive injury, suggesting global alterations cortical networks. Using transparent polymer skulls, we...
ABSTRACT Electrophysiological and optical imaging provide complementary neural sensing capabilities – electrophysiological recordings have the highest temporal resolution, while allows recording activities of genetically defined populations at high spatial resolution. Combining these complementary, yet orthogonal modalities to perform simultaneous large-scale, multimodal activity across multiple brain regions would be very powerful. Here we show that transparent, inkjet-printed...