A5090761140- Genetic factors in colorectal cancer
- Colorectal Cancer Treatments and Studies
- Cancer Genomics and Diagnostics
- Epigenetics and DNA Methylation
- Neurofibromatosis and Schwannoma Cases
- COVID-19 and Mental Health
- Suicide and Self-Harm Studies
- Computational Drug Discovery Methods
- GDF15 and Related Biomarkers
- Nutrition, Genetics, and Disease
- Bladder and Urothelial Cancer Treatments
- Colorectal Cancer Screening and Detection
- Neuroblastoma Research and Treatments
- RNA modifications and cancer
- Sarcoma Diagnosis and Treatment
- Child and Adolescent Psychosocial and Emotional Development
- Radiomics and Machine Learning in Medical Imaging
- PI3K/AKT/mTOR signaling in cancer
- Ferroptosis and cancer prognosis
- Long-Term Effects of COVID-19
- Pharmacogenetics and Drug Metabolism
- Hepatocellular Carcinoma Treatment and Prognosis
- Digital Mental Health Interventions
- Macrophage Migration Inhibitory Factor
- Cancer-related Molecular Pathways
Danish National Centre for Social Research
2023-2025
Gentofte Hospital
2024
University of Copenhagen
2021-2024
Oslo University Hospital
2010-2019
Molecular Oncology (United States)
2018
University of Oslo
1990-2017
Norwegian Cancer Society
2008-2017
Cancer Registry of Norway
2016
University Medical Center Groningen
2014
Istituto Ortopedico Rizzoli
2014
Cell lines are invaluable biomedical research tools, and recent literature has emphasized the importance of genotype authentication characterization. In present study, 24 out 27 cell line identities were confirmed by short tandem repeat profiling. The molecular phenotypes colon cancer examined, microsatellite instability (MSI) CpG island methylator phenotype (CIMP) determined, using Bethesda panel mononucleotide loci two epimarker panels, respectively. Furthermore, BRAF, KRAS PIK3CA...
Colorectal cancer (CRC) cell lines are widely used pre-clinical model systems. Comprehensive insights into their molecular characteristics may improve selection for biomedical studies. We have performed DNA, RNA and protein profiling of 34 lines, including (i) targeted deep sequencing (n = 612 genes) to detect single nucleotide variants insertions/deletions; (ii) high resolution DNA copy number profiling; (iii) gene expression at exon resolution; (iv) small by sequencing; (v) analysis 297...
Purpose: Response to standard oncologic treatment is limited in colorectal cancer. The gene expression-based consensus molecular subtypes (CMS) provide a new paradigm for stratified and drug repurposing; however, discovery currently by the lack of translation CMS preclinical models.Experimental Design: We analyzed primary cancers, cell lines, patient-derived xenografts (PDX). For classification models, we developed an optimized classifier enriched cancer cell-intrinsic expression signals,...
TNM staging alone does not accurately predict outcome in colon cancer (CC) patients who may be eligible for adjuvant chemotherapy. It is unknown to what extent the molecular markers microsatellite instability (MSI) and mutations BRAF or KRAS improve prognostic estimation multivariable models that include detailed clinicopathological annotation.After imputation of missing at random data, a subset accrued phase 3 trials with chemotherapy (n = 3016)-N0147 (NCT00079274) PETACC3 (NCT00026273)-was...
The evidence on mental health during COVID-19 evolved fast, but still little is known about the long-lasting impact of sequential lockdowns. We examine changes in young people's from before to initial and second more prolonged lockdown, whether women those with pre-existing depressive symptoms were disproportionally impacted.Participants reported indicators an ongoing 18-year data collection Danish National Birth Cohort a survey, including 8 points: 7 1 year post. Changes quality life (QoL),...
To identify a panel of epigenetic biomarkers for accurate bladder cancer (BlCa) detection in urine sediments.Gene expression microarray analysis BlCa cell lines treated with 5-aza-2'-deoxycytidine and trichostatin A as well 26 tissue samples was used to list novel methylation candidates BlCa. Methylation levels candidate genes were quantified 4 lines, 50 tissues, 20 normal mucosas (NBM), sediments from 51 patients healthy donors, 19 renal patients, prostate patients. Receiver operator...
Several microRNAs (miRNAs) are known to be deregulated in colon cancer, but the mechanisms behind their potential involvement on proliferation and tumor cell survival unclear. The present study aimed identify miRNAs with functional implications for development of cancer. apoptosis were examined following perturbations miRNA levels by employing a comprehensive library screen. nominated relevance cancer validated expression levels. By integrating effect up-regulation endogenous within HT29,...
Abstract Background The presence of cancer-specific DNA methylation patterns in epithelial colorectal cells human feces provides the prospect a simple, non-invasive screening test for cancer and its precursor, adenoma. This study investigates panel epigenetic markers detection adenomas. Methods Candidate biomarkers were subjected to quantitative analysis sets tissue samples from cancers, adenomas, normal colonic mucosa. All findings verified independent clinical validation series. A total...
The protease inhibitor cystatin C is a non-glycosylated low molecular weight protein (Mr=13359) which produced by all nucleated cells at constant rate, freely filtered the renal glomeruli, and catabolized in tubuli. aim of study was to elucidate applicability serum as marker glomerular filtration rate (GFR) patients with various kidney diseases wide range function dialysis patients. Seventy-six (aged 20 79 years) 61 21 82 were included. Serum measured automated particle-enhanced...
The incidence of colorectal cancer (CRC) increases with age and early onset indicates an increased likelihood for genetic predisposition this disease. somatic genetics tumor development in relation to patient remains mostly unknown. We have examined the mutation status five known critical genes at diagnosis, compared genomic complexity tumors from young patients without CRC syndromes those elderly patients. Among 181 patients, stratified by microsatellite instability status, DNA sequence...
Malignant peripheral nerve sheath tumours (MPNSTs) are a malignancy occurring with increased frequency in patients neurofibromatosis type 1 (NF1). In contrast to the well-known spectrum of germline NF1 mutations, information on somatic mutations MPNSTs is limited. this study, we screened NF1, KRAS, and BRAF 47 from (n = 25) without 22) NF1. addition, DNA blood cutaneous neurofibroma biopsies from, respectively, 14/25 7/25 were analysed. Germline detected ten patients, including three...
The three main types of urological cancers are mostly curable by surgical resection, if early detected. We aimed to identify novel DNA methylation biomarkers common these cancers, potentially suitable for non-invasive testing. From a candidate list markers created after gene expression assessment pharmacologically treated cell lines and tissue samples, two genes were selected further validation. Methylation levels quantified in total 12 cancer 318 clinical samples. PCDH17 TCF21 provided...
Approximately 15% of primary colorectal cancers have DNA mismatch repair deficiency, causing a complex genome with thousands small mutations-the microsatellite instability (MSI) phenotype. We investigated molecular heterogeneity and tumor immunogenicity in relation to clinical endpoints within this distinct subtype cancers.
BackgroundThe prognostic impact of KRAS and BRAFV600E mutations in primary colorectal cancer (CRC) varies with microsatellite instability (MSI) status. The gene expression–based consensus molecular subtypes (CMSs) CRC define molecularly clinically distinct subgroups, represent a novel stratification framework biomarker analysis. We investigated the value these within CMS groups.Patients methodsTotally 1197 tumors from Norwegian series stage I–IV were analyzed for MSI mutation status hotspots...
Colorectal cancer is a common disease with high mortality. Suitable biomarkers for detection of tumors at an early curable stage would significantly improve patient survival. Here, we show that the SPG20 (spastic paraplegia-20) promoter, encoding multifunctional Spartin protein, hypermethylated in 89% colorectal carcinomas, 78% adenomas and only 1% normal mucosa samples. methylation was also present pilot series stool samples corresponding from patients. promoter hypermethylation resulted...
Colorectal cancer (CRC) is one of the most common types in developed countries. To identify molecular networks and biological processes that are deregulated CRC compared to normal colonic mucosa, we applied Gene Set Enrichment Analysis two independent transcriptome datasets, including a total 137 ten mucosa samples. Eighty-two gene sets as described by Kyoto Encyclopedia Genes Genomes database had significantly altered expression both datasets. These included associated with cell division,...
The prognostic value of CpG island methylator phenotype (CIMP) in colorectal cancer remains unsettled. We aimed to assess the this analyzing a total 1126 tumor samples obtained from two Norwegian consecutive series. CIMP status was determined by 5-markers CAGNA1G, IGF2, NEUROG1, RUNX3 and SOCS1 quantitative methylation specific PCR (qMSP). effect on time recurrence (TTR) overall survival (OS) were uni- multivariate analyses. Subgroup analyses conducted according MSI BRAF mutation status,...
More than half of all colorectal carcinomas are known to exhibit an activated mitogen-activated protein kinase pathway. The NF1 gene, a negative regulator KRAS, has not previously been examined in series cancer. In the present study, primary stratified according microsatellite instability status were analyzed. whole coding region was analyzed for mutations using denaturing high-performance liquid chromatography and sequencing, copy number alterations multiple ligation-dependent probe...
Gap junctions are specialized plasma membrane domains consisting of channels formed by members the connexin protein family. junctional intercellular communication is often lost in cancers due to aberrant localization or downregulation connexins, and connexins therefore suggested act as tumor suppressor genes various tissues. The aim this study was investigate expression pattern DNA promoter methylation status colorectal cancer. Expression six (GJA1, GJA9, GJB1, GJB2, GJC1 GJD3) detected...
Abstract TP53 mutations are common in colorectal cancer (CRC). Most sequencing studies have been restricted to coding regions, but recent revealed that splice can generate transcript variants with distinct tumorigenic and prognostic properties. Here, we performed unrestricted of all sequences regions a single-hospital series 401 primary CRCs. were detected 4% the cases ( N = 16), considerably more frequent than reported major databases, they mutually exclusive exon mutations. RNA high-level...
BackgroundThe prevalence and clinical implications of genetic heterogeneity in patients with multiple colorectal liver metastases remain largely unknown. In a prospective series undergoing resection metastases, the aim was to investigate inter-metastatic primary-to-metastatic mutations KRAS, NRAS, BRAF, PIK3CA their prognostic impact.Patients MethodsWe analyzed mutation status among 372 78 primary tumors from 106 by methods used routine testing, Sanger sequencing, next-generation sequencing...