A5014897747- Gut microbiota and health
- Alcohol Consumption and Health Effects
- Barrier Structure and Function Studies
- Helicobacter pylori-related gastroenterology studies
- Nanoplatforms for cancer theranostics
- Dietary Effects on Health
- Effects of Radiation Exposure
- Drug Transport and Resistance Mechanisms
- Cancer Research and Treatments
- Connexins and lens biology
- Tryptophan and brain disorders
- Prenatal Substance Exposure Effects
- Ion Channels and Receptors
- Birth, Development, and Health
- Long-Term Effects of COVID-19
- Neuroinflammation and Neurodegeneration Mechanisms
- Diet and metabolism studies
- Melanoma and MAPK Pathways
- Gastric Cancer Management and Outcomes
- Nanoparticle-Based Drug Delivery
- Neurological Disease Mechanisms and Treatments
- Biochemical effects in animals
- Medical and Biological Ozone Research
- Liver Disease Diagnosis and Treatment
- COVID-19 Clinical Research Studies
All India Institute of Medical Sciences
2023-2025
Department of Biotechnology
2023-2025
University of Tennessee Health Science Center
2015-2024
Centre for Cellular and Molecular Biology
2020-2022
National Institute of Pathology
2015-2016
National Centre for Cell Science
2010-2014
Savitribai Phule Pune University
2012
National Brain Research Centre
2007
Despite modern advances in treatment, skin cancer is still one of the most common causes death western countries. Chemotherapy plays an important role melanoma management. Tamoxifen has been used either alone or in- combination with other chemotherapeutic agents to treat melanoma. However, response rate tamoxifen as a single agent comparatively low. In present study, we investigated whether treatment methyl-β-cyclodextrin (MCD), cholesterol depleting agent, increases efficacy cells. This was...
The goals of this study were to evaluate the effects ionizing radiation on apical junctions in colonic epithelium and mucosal barrier function mice vivo. Adult subjected total body irradiation (4 Gy) with or without N-acetyl-l-cysteine (NAC) feeding for 5 days before irradiation. At 2-24 h postirradiation, integrity epithelial tight (TJ), adherens (AJ), actin cytoskeleton was assessed by immunofluorescence microscopy immunoblot analysis detergent-insoluble fractions TJ AJ proteins. evaluated...
Hepatocellular carcinoma (HCC) is a primary malignancy of the liver and major cause cancer related deaths worldwide. Only 10 to 20% HCC can be surgically excised. Therefore, chemotherapeutic intervention treatment essential for achieving favorable prognosis. However, therapeutic outcome chemotherapy generally poor owing inherent resistance cells or due development acquired resistance. To differentiate delineate molecular events, we developed drug resistant Hep3B (DRC) by treating with...
The role of reactive oxygen species (ROS) in osmotic stress, dextran sulfate sodium (DSS) and cyclic stretch-induced tight junction (TJ) disruption was investigated Caco-2 cell monolayers vitro restraint stress-induced barrier dysfunction mouse colon vivo Live imaging showed that stretch DSS triggered rapid production ROS monolayers, which blocked by depletion intracellular Ca2+ 1,2-bis-(o-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid. Knockdown CaV1.3 or TRPV6 channels stress DSS-induced...
// Balkrishna Chaube 1 , Parmanand Malvi Shivendra Vikram Singh Naoshad Mohammad Avtar Meena 1,2 and Manoj Kumar Bhat National Centre for Cell Science, Savitribai Phule Pune University Campus, Ganeshkhind, Pune, India 2 Current address: Department of Physiology, Tennessee Health Science Center, Memphis, USA Correspondence to: Bhat, email: Keywords : melanoma; complex I; LDH; metabolic catastrophe; synthetic lethality Received June 16, 2015 Accepted September 23, Published October 15,...
Abstract Alcohol use disorders are associated with altered stress responses, but the impact of or hormones on alcohol-associated tissue injury remain unknown. We evaluated effects chronic restraint alcohol-induced gut barrier dysfunction and liver damage in mice. To determine whether corticosterone is hormone stress-induced effects, we effect treatment alcoholic at Gut-Liver-Brain (GLB) axis. Chronic synergized epithelial tight junction disruption mucosal mouse intestine. These were...
Intestinal epithelial tight junction disruption is a primary contributing factor in alcohol-associated endotoxemia, systemic inflammation, and multiple organ damage. Ethanol acetaldehyde disrupt junctions by elevating intracellular Ca2+. Here we identify TRPV6, Ca2+-permeable channel, as responsible for alcohol-induced elevation of Ca2+, intestinal barrier dysfunction, inflammation. elicit TRPV6 ionic currents Caco-2 cells. Studies cell monolayers mouse organoids show that deficiency or...
The spectrum of health complications instigated by coronavirus disease 2019 (COVID-19, caused the novel severe acute respiratory syndrome 2 or SARS-CoV-2) pandemic has been diverse and complex. Besides evident pulmonary cardiovascular threats, accumulating clinical data points to several neurological complications, which are more common in elderly COVID-19 patients. Recent pieces evidence have marked events neuro infection neuroinvasion, producing patients; however, a systematic...
Pathogenesis of alcohol-related diseases such as alcoholic hepatitis involves gut barrier dysfunction, endotoxemia, and toxin-mediated cellular injury. Here we show that Lactobacillus plantarum not only blocks but also mitigates ethanol (EtOH)-induced liver damage in mice. L. EtOH-induced protein thiol oxidation, down-regulation antioxidant gene expression colon EtOH- induced TNF-α, IL-1β, IL-6, monocyte chemotactic 1 (MCP1), C-X-C motif chemokine ligand (CXCL)1, CXCL2 genes colon. Epidermal...
Alcohol consumption has been shown to cause dysbiosis, but the mechanism involved in it is unknown. Recurrent colitis known induce expression of α-defensins colon, effect alcohol on not known. We investigated ethanol α-defensin small intestine and colitis-induced colon mice. Furthermore, we evaluated human defensin-5 (HD5) gut barrier dysfunction mucosal damage. was induced by feeding dextran sulfate sodium (DSS), 3 cycles 5-days each with 15 days intervals, followed 30-days remission....
Abstract Ethanol is metabolized into acetaldehyde in most tissues. In this study, we investigated the synergistic effect of ethanol and on tight junction integrity Caco-2 cell monolayers. Expression alcohol dehydrogenase sensitized cells to ethanol-induced disruption barrier dysfunction, whereas aldehyde attenuated acetaldehyde-induced disruption. up 150 mM did not affect or function, but it dose-dependently increased acetaldehyde-mediated dysfunction. Src kinase MLCK inhibitors blocked...
Corticosterone, the stress hormone, exacerbates alcohol-associated tissue injury, but mechanism involved is unknown. We examined role of glucocorticoid receptor (GR) in corticosterone-mediated potentiation alcohol-induced gut barrier dysfunction and systemic response. Hepatocyte-specific GR-deficient (GRΔHC) intestinal epithelial-specific (GRΔIEC) mice were fed ethanol, combined with corticosterone treatment. Intestinal epithelial tight junction integrity, mucosal function, microbiota...
Alcohol consumption is one of the major risk factors for colorectal cancer. However, mechanism involved in this effect alcohol unknown. We evaluated chronic ethanol feeding on azoxymethane and dextran sulfate sodium (AOM/DSS)-induced carcinogenesis mouse colon. Inflammation colonic mucosa was assessed at a precancerous stage by evaluating mucosal infiltration neutrophils macrophages, analysis cytokine chemokine gene expression. Chronic significantly increased number size polyps colon AOM/DSS...
Artificial insemination (AI) has transformed poultry reproduction by enabling genetic improvement, enhancing productivity, and addressing challenges such as disease transmission mating inefficiencies. This method involves manually depositing semen into the female reproductive tract, maximizing utilization of genetically superior males. AI is particularly valuable in overcoming size disparities broilers, optimizing specialized breeds, preserving endangered avian species. Semen collected using...