A5036667980- Bacterial Infections and Vaccines
- Pneumonia and Respiratory Infections
- Bacterial Genetics and Biotechnology
- Genomics and Phylogenetic Studies
- RNA and protein synthesis mechanisms
- Antibiotic Resistance in Bacteria
- Listeria monocytogenes in Food Safety
- Infective Endocarditis Diagnosis and Management
- Bacterial biofilms and quorum sensing
- Respiratory viral infections research
- Tuberculosis Research and Epidemiology
- Pleural and Pulmonary Diseases
- Transgenic Plants and Applications
- Mycobacterium research and diagnosis
- Antimicrobial Resistance in Staphylococcus
- Vibrio bacteria research studies
- Bacillus and Francisella bacterial research
- SARS-CoV-2 and COVID-19 Research
- Viral Infectious Diseases and Gene Expression in Insects
- Cancer Research and Treatments
- SARS-CoV-2 detection and testing
- Streptococcal Infections and Treatments
- Viral gastroenteritis research and epidemiology
- Microbial Metabolism and Applications
- Orthopaedic implants and arthroplasty
University of Würzburg
2015-2025
BioTeSys (Germany)
2025
Institute of Medical Microbiology and Hygiene
2008-2024
St.Petersburg V.M.Bekhterev Psychoneurological Research Institute
2016
Rafael Advanced Defense Systems (Israel)
2016
Universität Hamburg
2016
Universitätsklinikum Würzburg
2016
Bernhard Nocht Institute for Tropical Medicine
2015-2016
University of Tübingen
2016
Eötvös Loránd University
2016
Cellular RNAs guide CRISPR-Cas9 The Cas9 nuclease widely used for genome editing is derived from natural bacterial defense systems that protect against invading viruses. directed by RNA guides to cut matching viral DNA. Jiao et al. discovered can also originate cellular unassociated with (see the Perspective Abudayyeh and Gootenberg). They rendered this process programmable, linking presence of virtually any cutting DNA Cas9. This capability basis a new CRISPR diagnostic method developed...
Neisseria meningitidis is a leading cause of infectious childhood mortality worldwide. Most research efforts have hitherto focused on disease isolates belonging to only few hypervirulent clonal lineages. However, up 10% the healthy human population temporarily colonized by genetically diverse strains mostly with little or no pathogenic potential. Currently, known about biology carriage and their evolutionary relationship isolates. The expression polysaccharide capsule trait that has been...
Parapneumonic pleural effusions/empyema (PPE/PE) are severe complications of community-acquired pneumonia. We investigated the bacterial aetiology and incidence paediatric PPE/PE in Germany after introduction universal pneumococcal conjugate vaccine (PCV) immunization for infants.Children <18 years age hospitalized with pneumonia-associated necessitating drainage or persisting >7 days were reported to German Surveillance Unit Rare Diseases Childhood between October 2010 June 2017. All...
Coagulase-negative staphylococci (CoNS) are common opportunistic pathogens, but also ubiquitous human and animal commensals. Infection-associated CoNS from healthcare environments typically characterized by pronounced antimicrobial resistance (AMR) including both methicillin- multidrug-resistant isolates. Less is known about AMR patterns of colonizing the general population. Here we report on in commensal recovered 117 non-hospitalized volunteers a region Germany with high livestock density....
ABSTRACT Mutants of Listeria monocytogenes with deletions in genes the common branch biosynthesis pathway leading to aromatic compounds were constructed as possible virulence-attenuated carrier strains for protein antigens or vaccine DNA. aroA , aroB and particular aroE mutants showed strongly reduced growth rates epithelial cells even rich culture media. The metabolism aro under these conditions was predominantly anaerobic. Aerobic a wild-type rate were, however, regained upon addition...
Background Neisseria meningitidis is a naturally transformable, facultative pathogen colonizing the human nasopharynx. Here, we analyze on genome-wide level impact of recombination gene-complement diversity and virulence evolution in N. meningitidis. We combined comparative genome hybridization using microarrays (mCGH) multilocus sequence typing (MLST) 29 meningococcal isolates with computational comparison subset seven sequences. Principal Findings found that lateral gene transfer minimal...
Neisseria meningitidis is a human commensal that can also cause life-threatening meningitis and septicemia. Despite growing evidence for RNA-based regulation in meningococci, their transcriptome structure output of regulatory small RNAs (sRNAs) are incompletely understood. Using dRNA-seq, we have mapped at single-nucleotide resolution the primary N. strain 8013. Annotation 1625 transcriptional start sites defines transcription units most protein-coding genes but reveals paucity classical...
Pediatric pneumococcal pneumonia complicated by parapneumonic pleural effusion/empyema (PPE/PE) remains a major concern despite general immunization with conjugate vaccines (PCVs).In nationwide pediatric hospital surveillance study in Germany we identified 584 children <18 years of age bacteriologically confirmed PPE/PE from October 2010 to June 2018. Streptococcus pneumoniae was culture and/or PCR blood samples fluid and serotyped.S. 256 (43.8%) (n = 122) 207). The following serotypes were...
ABSTRACT Neisseria meningitidis serogroup B strains are responsible for most meningococcal cases in the industrialized countries, and belonging to clonal complex ST-41/44 among prevalent carriage disease. Here, we report first genome transcriptome comparison of a strain from disease MC58 ST-32. Both genomes highly colinear, with only three major rearrangements that associated integration mobile genetic elements. They further differ about 10% their gene content, highest variability presence...
Zinc is a bivalent cation essential for bacterial growth and metabolism. The human pathogen Neisseria meningitidis expresses homologue of the uptake regulator Zur, which has been postulated to repress putative zinc protein ZnuD. In this study, we elucidated transcriptome meningococci in response by microarrays quantitative real-time PCR (qRT-PCR). We identified 15 genes that were repressed two activated upon addition. All transcription units (genes operons) harbored Zur binding motif their...
Summary Objective: To evaluate the initial management of pediatric parapneumonic effusion or pleural empyema (PPE/PE) with regard to length hospital stay (LOS). Methods: Collection PPE/PE cases using a nationwide surveillance system (ESPED) from 10/2010 06/2013, in all German hospitals. Inclusion patients <18 years age requiring drainage persistence >7 days. Staging based on reported sonographic imaging. Comparison LOS after diagnosis between children treated different forms invasive...
FinO-domain proteins are a widespread family of bacterial RNA-binding with regulatory functions. Their target spectrum ranges from single RNA pair, in the case plasmid-encoded FinO, to global regulons, as enterobacterial ProQ. To assess whether FinO domain itself is intrinsically selective or promiscuous, we determine vivo targets Neisseria meningitidis, which consists solely domain. UV-CLIP-seq identifies associations 16 small non-coding sRNAs and 166 mRNAs. Meningococcal ProQ predominantly...
<h3>Importance</h3> Closure of day care centers has been implemented globally to contain the COVID-19 pandemic but negative effects on children's health and psychosocial well-being. <h3>Objective</h3> To investigate feasibility surveillance among children childcare workers model efficacy viral spread prevention. <h3>Design, Setting, Participants</h3> This nonrandomized controlled trial was conducted at 9 in Wuerzburg, Germany, from October 2020 March 2021. Participants included attending...
This study investigated empiric antibiotic treatment (EAT), guideline adherence, streamlining and clinical outcomes in 1402 hospitalized children with pediatric parapneumonic effusion/pleural empyema (PPE/PE).
Abstract Visceral pentastomiasis caused by Armillifer armillatus larvae was diagnosed in 2 dogs The Gambia. Parasites were subjected to PCR; phylogenetic analysis confirmed relatedness with branchiurans/crustaceans. Our investigation highlights transmission of infective A. ova and, serologic evidence, also 1 human, demonstrating a public health concern.
Some members of the physiological human microbiome occasionally cause life-threatening disease even in immunocompetent individuals. A prime example such a commensal pathogen is Neisseria meningitidis, which normally resides nasopharynx but also leading sepsis and epidemic meningitis. Using N. meningitidis as model organism, we tested hypothesis that virulence pathogens consequence within host evolution selection invasive variants due to mutations at contingency genes, mechanism called phase...