A5023349336- Urinary Bladder and Prostate Research
- Hormonal and reproductive studies
- Nitric Oxide and Endothelin Effects
- Advanced Glycation End Products research
- Sexual function and dysfunction studies
- Pelvic floor disorders treatments
- Urinary Tract Infections Management
- Pharmacological Effects and Toxicity Studies
- Renin-Angiotensin System Studies
- Connexins and lens biology
- Hemoglobinopathies and Related Disorders
- Tryptophan and brain disorders
- Ion Channels and Receptors
- Circadian rhythm and melatonin
- Aldose Reductase and Taurine
- Phytoestrogen effects and research
- Hormonal Regulation and Hypertension
- Thermoregulation and physiological responses
- Dermatology and Skin Diseases
- Blood Pressure and Hypertension Studies
- Cardiac Ischemia and Reperfusion
- Drug Transport and Resistance Mechanisms
- Receptor Mechanisms and Signaling
- Pharmacogenetics and Drug Metabolism
- Amino Acid Enzymes and Metabolism
Universidade Estadual de Campinas (UNICAMP)
2015-2024
Universidade São Francisco
2024
Universidade de São Paulo
2024
Hospital Universitário - Universidade Federal de Juiz de Fora
2023
Universidade Federal do Rio Grande
2020
Hospital de Clínicas da Unicamp
2012
Methylglyoxal (MGO) is a highly reactive α-dicarbonyl compound formed endogenously from 3-carbon glycolytic intermediates. accumulated in plasma and urine of hyperglycemic diabetic individuals acts as potent peptide glycation molecule, giving rise to advanced end products (AGEs) like arginine-derived hydroimidazolone (MG-H1) carboxyethyl-lysine (CEL). Methylglyoxal-derived AGEs exert their effects mostly via activation RAGE, cell surface receptor that initiates multiple intracellular...
Abstract The aim of this study was to develop a high‐performance liquid chromatography–tandem mass spectrometry method for the determination 6‐cyanodopamine, 6‐nitrodopamine, 6‐nitrodopa, 6‐nitroadrenaline and 6‐bromodopamine in human plasma samples. Strata‐X 33 μm solid‐phase extraction cartridges were used catecholamines from separated 150 × 3 mm Shim‐pack GIST C 18 ‐AQ column with particle size, placed an oven at 40°C perfused 82% mobile phase A (acetonitrile–H 2 O; 90:10, v/v) + 0.4%...
Activators of soluble guanylyl cyclase (sGC) interact directly with its prosthetic heme group, enhancing the enzyme responsiveness in pathological conditions. This study aimed to evaluate effects sGC activator BAY 58-2667 on voiding dysfunction, protein expressions α1 and β1 subunits cGMP levels bladder tissues after cyclophosphamide (CYP) exposure. Female C57BL/6 mice (20-25 g) were injected CYP (300 mg/kg ip) induce cystitis. Mice pretreated or not (1 mg/kg, gavage), given 1 h before...
Methylglyoxal (MGO) is a highly reactive dicarbonyl compound implicated in diabetes-associated diseases. In vascular tissues, MGO induces the formation of advanced glycation end products (AGEs) that bounds its receptor RAGE, initiating downstream tissue injury. Outside cardiovascular system, intake produces mouse voiding dysfunction and bladder overactivity. We have sought MGO-induced overactivity due to activation AGE-RAGE-reactive-oxygen species (ROS) signaling cascade, leading Rho kinase...
Introduction: The human umbilical artery (HUA), rat-isolated right atrium, and vas deferens present a basal release of 6-nitrodopamine (6-ND). 6-ND from these tissues was significantly decreased (but not abolished) when the were pre-incubated with N ω -nitro-L-arginine methyl ester (L-NAME). Methods: In this study, effect pharmacological modulation redox environment on investigated. measured using Liquid chromatography tandem mass spectrometry (LC-MS/MS). Results Discussion: Pre-incubation...
Urethral smooth muscle (USM) contributes to urinary continence by contracting during the urine storage phase, which is mainly mediated activation of postjunctional α 1 -adrenoceptors. Males and females show differences in functioning lower tract most common symptoms (LUTS). LUTS men typically occur association with bladder outlet obstruction, whereas women urge-incontinence are more common. Therefore, this study aimed evaluate sex -adrenoceptor subtype expression their importance proximal...
Interstitial cystitis/bladder pain syndrome (IC/BPS) is a chronic inflammatory disease without consistently effective treatment. Among the many mediators implicated in cystitis, overproduction of reactive oxygen species (ROS) seems to play key role, although main source ROS remains unclear. This study aimed investigate contribution NADPH oxidase (NOX) isoforms generation and voiding dysfunction cyclophosphamide (CYP, 300 mg/Kg, ip, 24 h)-induced cystitis adult female mice, well-recognized...
Aims To evaluate the effects of beta‐3 adrenoceptor agonist, mirabegron in a mouse model detrusor overactivity induced by obesity. Methods C57BL/6 male mice were fed with standard chow or high‐fat diet for 12 weeks. Lean and obese treated orally (10 mg/kg/day) from last 2 weeks diet. Cystometric evaluations, functional assays, protein expression phosphodiesterase type 4 (PDE4), cyclic adenosine monophosphate (cAMP) measurement carried out. Results In body weight, epididymal fat mass, fasting...
Interstitial Cystitis/Bladder Pain Syndrome (IC/BPS) is a chronic inflammatory disease without consistently effective treatment. We investigate the role of toll-like receptor 4 (TLR4) on voiding dysfunction and inflammation in cyclophosphamide (CYP)-induced mouse cystitis. Male C57BL/6 [wild-type, (WT)] and/or TLR4 knockout (TLR4 −/− ) mice were treated with an injection CYP (300 mg/kg, 24 h) or saline (10 ml/kg). The pharmacological blockade by resatorvid mg/kg) was also performed 1 h prior...
Androgen deficiency has been implicated in urological complications of postmenopausal women. This study examined the effects testosterone replacements on lower urinary tract dysfunction 4-month old ovariectomized (OVX) rats.Sprague-Dawley female rats were OVX bilaterally. Three months later, received single intramuscular injections undecanoate. Cystometric study, and bladder urethra smooth muscle reactivities evaluated.Ovariectomy reduced by 65% (p<0.05) serum levels. Testosterone...
The biologic effect of cAMP and cGMP is terminated by phosphodiesterases multidrug resistance proteins MRP4 MRP5, which pump cyclic nucleotides out the cell. Therefore, this study aimed to characterize role MRP inhibitor, MK 571 (3-[[[3-[(1<i>E</i>)-2-(7-chloro-2-quinolinyl)ethenyl]phenyl][[3-(dimethylamino)-3-oxopropyl]thio]methyl]thio]propanoic acid), in bladder, prostate, urethra male mice means functional assays, protein expression, nucleotide quantification. cumulative addition (1–30...
Diabetic bladder dysfunction (DBD) is one of the most common complication diabetes. Methylglyoxal (MGO), a highly reactive dicarbonyl compound formed as by-product glycolysis, found at high levels in plasma diabetic patients. Here, we explored effects chronic administration MGO on micturition pattern (cystometry) and contractility vitro healthy male C57/BL6 mice. was given 0.5% drinking water for 4 weeks. Exposure to led tissue disorganization, edema lamina propria, partial loss urothelium...
Methylglyoxal (MGO) is a highly reactive dicarbonyl species implicated in diabetic-associated diseases. Acute lung injury (ALI) symptoms and prognosis are worsened by diabetes obesity. Here, we hypothesized that elevated MGO levels aggravate ALI, which can be prevented metformin. Therefore, this study evaluated the inflammation lipopolysaccharide (LPS)-exposed mice pretreated with MGO.C57Bl/6 male treated or not for 12 weeks were intranasally instilled LPS (30 µg) to induce metformin (300...
To evaluate the functional and molecular alterations of contractile relaxant machinery in bladder urethra that lead to underactive (UAB) old female mice.Female young (3-months) (18-months) C57BL/6 mice were used. Urodynamic was assessed awake anaesthetized mice. Electrical-field stimulation (EFS) concentration-response curves relaxing agents isolated bladders urethras performed. Messenger RNA (mRNA) expressions muscarinic, adrenergic, transient receptor potential vanilloid-4 (TRPV4), enzymes...
Background: The prostate gland is surrounded by periprostatic adipose tissue (PPAT) that can release mediators interfere in function. In this study, we examined the effect of supernatant obtained from obese mice on reactivity vitro and viability human prostatic epithelial cell lines. Methods: Male C57BL/6 were fed a standard or high-fat diet after which PPAT was isolated, incubated Krebs-Henseleit solution for 30 min (without prostate) 60 (with prostate), then collected screened biological...