A5080930097- Enzyme Catalysis and Immobilization
- Antibiotic Resistance in Bacteria
- Enzyme Production and Characterization
- Microbial Metabolic Engineering and Bioproduction
- Biofuel production and bioconversion
- Chemistry and Chemical Engineering
- Microbial Natural Products and Biosynthesis
- biodegradable polymer synthesis and properties
- Analytical Chemistry and Chromatography
- Studies on Chitinases and Chitosanases
- Microbial Applications in Construction Materials
- Computational Drug Discovery Methods
- Bacteriophages and microbial interactions
- Vibrio bacteria research studies
- Bacterial Identification and Susceptibility Testing
- Immune responses and vaccinations
- Antibiotic Use and Resistance
- Folate and B Vitamins Research
- Cancer Research and Treatments
- Pharmaceutical and Antibiotic Environmental Impacts
- Viral gastroenteritis research and epidemiology
- Microbial Community Ecology and Physiology
- Bacterial Infections and Vaccines
- Pharmacogenetics and Drug Metabolism
- Iron Metabolism and Disorders
Mutah University
2019-2024
Beni-Suef University
2014-2024
Al-Azhar University
2023
Saad Specialist Hospital
2023
University of Kara
2019
Academy of Scientific Research and Technology
2018
Helwan University
2017
Lund University
2010-2015
University of Groningen
2014
A simple and efficient approach for profiling the greenness of high performance liquid chromatography (HPLC) methods is presented. This environmental assessment tool (EAT) takes into consideration environmental, health safety issues all solvents involved in chromatographic method, calculates a total score that can be used comparison different methods. software, HPLC-EAT, has been designed to facilitate calculation downloaded free charge at http://www.biotek.lu.se/hplc-eat/. HPLC-EAT was...
Most of the current colorimetric methods for detection chitinase or cellulase activities on insoluble natural polymers chitin and cellulose depend a chemical redox reaction. The reaction involves reducing ends hydrolytic products. Schales' procedure 3,5-dinitrosalicylic acid (DNS) method are two examples that commonly used. However, these lack sensitivity present practical difficulties usage in high-throughput screening assays as they require boiling heating steps color development.We report...
Abstract Fungal endophytes are a major source of anti-infective agents and other medically relevant compounds. However, their classical blinded-chemical investigation is challenging process due to highly complex chemical makeup. Thus, utilizing cheminformatics tools such as metabolomics computer-aided modelling great help deal with complexity select the most probable bioactive candidates. In present study, we have explored fungal associated well-known antimalarial medicinal plant Artemisia...
The prevalence of extensively and pandrug-resistant strains Acinetobacter baumannii leaves little or no therapeutic options for treatment this bacterial pathogen. Bacteriophages their lysins represent attractive alternative antibacterial strategies in regard. We used the drug-resistant A. strain MK34 to isolate bacteriophage PMK34 (vB_AbaP_PMK34). This phage shows fast adsorption lacks virulence genes; nonetheless, its narrow host spectrum based on capsule recognition limits broad...
Since the emergence of SARS-CoV-2 pandemic in 2019, it has remained a significant global threat, especially with newly evolved variants. Despite presence different COVID-19 vaccines, discovery proper antiviral therapeutics is an urgent necessity. Nature considered as historical trove for drug discovery, crises. During our efforts to discover potential anti-SARS CoV-2 natural therapeutics, screening in-house products and plant crude extracts library led identification C. benedictus extract...
The rising antimicrobial resistance is particularly alarming for Acinetobacter baumannii, calling the discovery and evaluation of alternatives to treat A. baumannii infections. Some bacteriophages produce a structural protein that depolymerizes capsular exopolysaccharide. Such purified depolymerases are considered as novel antivirulence compounds. We identified characterized depolymerase (DpoMK34) from phage vB_AbaP_PMK34 active against clinical isolate MK34. In silico analysis reveals...
The diversity of actinomycetes associated with the marine sponge Coscinoderma mathewsi collected from Hurghada (Egypt) was studied. Twenty-three were separated and identified based on 16S rDNA gene sequence analysis. Out them, three isolates classified as novel species genera Micromonospora, Nocardia, Gordonia. Genome sequencing actinomycete strains has revealed many silent biosynthetic clusters shown their exceptional capacity for production secondary metabolites, not observed under...
Phage lysins are a new class of enzyme-based antibiotics that increasingly gain interest. Lysins kill cells through rapid degradation the peptidoglycan layer, resulting in sudden osmotic lysis.
The<italic>lactobacillus casei</italic>and inulin modulate the expression of JNK-1, GSK3β and β-catenin proteins enrich beneficial bacteria to protect from colon cancer in mice.
Abstract Background Some nonsteroidal anti-inflammatory drugs (NSAIDs) have been shown to antibacterial activities in different experimental settings. In the current study, of meloxicam and diclofenac were evaluated when combined with curcumin, a polyphenolic compound, against clinically relevant bacteria. The aim was determine whether these combinations could provide novel, synergistic approach combat pathogenic Results minimum inhibitory concentration (MIC) values curcumin (CCM), (MXM),...
Abstract Background Polycyclic aromatic hydrocarbons (PAHs) such as anthracene are one of the most toxic contaminants to our environment. Microbial biodegradation these xenobiotics is a cost-effective technological solution. The present study aimed recover some bacterial isolates from Beni-Suef Governorate in Egypt with high capabilities biodegradation. selected were molecularly characterized by 16S rRNA gene sequencing, degree was monitored using optical density (OD) and high-performance...
A biocatalysis based method for the solvent-free production of N-alkanoyl-N-methylglucamide (MEGA) surfactants was developed and used as a case study evaluation different environmental assessment tools, such freeware package EATOS (Environmental Assessment Tool Organic Synthesis). In order to also consider energy usage process facilities, e.g. heating, stirring vacuum, complementary tool needed; hence EcoScale use an monitoring socket were exploited. The followed by simple hydrolysis step...
Microbial geotechnology is the use of microorganisms and/or their derivatives to alter engineering properties soil for improving its stability, strength and stiffness. Ureases hydrolyze urea in leading CaCO3 precipitation, which binds particles together (biocementation). In present study, nine Egyptian soils were screened urease-producing bacteria, 15 isolates obtained, optimum urease producers identified. Growth kinetics measured at different pH values presence molasses as sole carbon...
Soils with poor bearing capacities represent a challenge to construction engineers, therefore continuous investigation of different additives and stabilizers is sought in order improve such weak soils reduce the cost replacement. In this paper, an experimental program adopted investigate effect environmentally friendly material based on enzymes soil performance. The dosages two commercial enzymatic preparations strength, maximum dry density permeability five was examined. Besides, California...
Ventilator-associated pneumonia (VAP) is a major health problem for people intubated in intensive care units (ICUs), leading to increased mortality rates, hospital stay, and treatment costs. In the present study, core pathogens causing VAP Beni-Suef University's Hospital, Egypt, was investigated over study period of 2 years (2017-2019).Of total 213 patients subjected mechanical ventilation, 60 have developed during their stay ICU. The rate reached 41.7% among patients. Sixty bacteria were...
Bio-based 5-hydroxymethylfurfural (HMF) serves as an important platform for several chemicals, among which 2,5-furan dicarboxylic acid (FDCA) has attracted considerable interest a monomer the production of polyethylene furanoate (PEF), potential alternative fossil-based terephthalate (PET). This study is based on HMF oxidizing activity shown by Mycobacterium sp. MS 1601 cells and investigation enzyme catalysing oxidation. The whole oxidized to FDCA (60% yield) hydroxymethyl furan carboxylic...
N-Lauroyl-N-methylglucamide is a biodegradable surfactant derived from renewable resources. In an earlier study, we presented enzymatic solvent-free method for synthesis of this compound. the present report, HPLC developed to follow reaction between lauric acid/methyl laurate and N-methyl glucamine (MEG) its environmental assessment are described.Use ultraviolet (UV) absorption or refractive index (RI) detectors did not allow detection (MEG). With Evaporative light scattering detector ELSD,...
A highly functionalized six-membered cyclic carbonate, methacrylated trimethylolpropane (TMP) which can be used as a potential monomer for bisphenol-free polycarbonates and isocyanate-free polyurethanes, was synthesized by two steps transesterifications catalyzed immobilized Candida antarctica lipase B, Novozym(®) 435 (N435) followed thermal cyclization. TMP 70 to 80% selectivity of mono-methacrylate with 70% conversion achieved, the reaction rate evaluated using various acyl donors such...
Background and Objectives: Methicillin-resistant Staphylococcus aureus (MRSA) is a major concern in Jordanian hospitals terms of infection control. The purpose this study was to identify the resistance patterns strains isolated from surfaces critical locations within Al-Karak Governmental Hospital 2019. Additionally, aimed conduct whole-genome sequencing on isolates. Materials Methods: In February 2019, fourteen S. were sites Hospital. These isolates underwent antibiogram testing determine...